Czech J. Anim. Sci., 2009, 54(7):293-306 | DOI: 10.17221/1724-CJAS

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

T. Krejčová1, J. Petr2, M. Krejčová1, K. Kheilová1
1 Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, Prague, Czech Republic
2 Research Institute of Animal Production, Prague-Uhříněves, Czech Republic

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P < 0.05). However, this developmental rate was significantly lower (P < 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). When we combined pulsatile SNAP-treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6-DMAP) (2mM 6-DMAP for 2 hours) or with the inhibitor of protein synthesis cycloheximide (CHX) (10 µM CHX for 2 hours), we observed a significant increase (P < 0.05) in the activation rate when compared to the respective pulsatile SNAP-treatment without 6-DMAP or CHX (63 vs. 78% of activated oocytes for 6-DMAP; 63 vs. 83% of activated oocytes for CHX). However, the development of parthenogenetic embryos was not enhanced when the pulsatile SNAP-treatment was combined with 6-DMAP or with CHX.

Keywords: activation; nitric oxide; oocyte; pig

Published: July 31, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Krejčová T, Petr J, Krejčová M, Kheilová K. Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor. Czech J. Anim. Sci. 2009;54(7):293-306. doi: 10.17221/1724-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Athanassakis I., Aifantis I., Baritakis S., Farmakiotis V., Koumantakis E., Vassiliadis S. (2000): Nitric oxide production by pre-implantation embryos in response to embryotoxic factors. Cellular Physiology and Biochemistry, 10, 169-76. Go to original source... Go to PubMed...
    2. Bussemer T., Otto I., Bodmeier R. (2001): Pulsatile drugdelivery systems. Critical Reviews in Therapeutic Drug Carrier Systems, 18, 433-58. Go to original source... Go to PubMed...
    3. Campbell S.C., Richardson H., Ferris W.F., Butler C.S., Macfarlane W.M. (2007): Nitric oxide stimulates insulin gene transcription in pancreatic beta-cells. Biochemical and Biophysical Research Communication, 353, 1011-1016. Go to original source... Go to PubMed...
    4. Collas P., Fissore R., Robl J.M., Sullivan E.J., Barnes F.L. (1993): Electrically induced calcium elevation, activation, and parthenogenetic development of bovine oocytes. Molecular Reproduction and Development, 34, 212-223. Go to original source... Go to PubMed...
    5. Dekel N. (2005): Cellular, biochemical and molecular mechanisms regulating oocyte maturation. Molecular and Cellular Endocrinology, 234, 19-25. Go to original source... Go to PubMed...
    6. Ducibella T., Huneau D., Angelichio E., Xu Z., Schultz R.M., Kopf G.S., Fissore R., Madoux S., Ozil J.P. (2002): Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number. Developmental Biology, 250, 280-291. Go to original source...
    7. Dupont G., Goldbeter A. (1998): CaM kinase II as frequency decoder of Ca 2+ oscillations. Bioessays, 20, 607-610. Go to original source... Go to PubMed...
    8. Fujimoto S., Yoshida N., Fukui T., Amanai M., Isobe T., Itagaki C., Izumi T., Perry A.C. (2004): Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix. Developmental Biology, 274, 370-383. Go to original source... Go to PubMed...
    9. Garban H.J., Marquez-Garban D.C., Pietras R.J., Ignarro L.J. (2005): Rapid nitric oxide-mediated S-nitrosylation of estrogen receptor: regulation of estrogen-dependent gene transcription. Proceedings of the National Academy of Sciences of United States of America, 102, 2632-2636. Go to original source... Go to PubMed...
    10. Gudmundsson A., Carnes M. (1997): Pulsatile adrenocorticotropic hormone: an overview. Biological Psychiatry, 41, 342-65. Go to original source... Go to PubMed...
    11. Guo K., Andres V., Walsh K. (1998): Nitric oxide-induced downregulation of Cdk2 activity and cyclin a gene transcription in vascular smooth muscle cells. Circulation, 97, 2066-2072. Go to original source... Go to PubMed...
    12. Hornick P., Taylor K. (1997): Pulsatile and nonpulsatile perfusion: the continuing controversy. Journal of Cardiothoracic and Vascular Anesthesia, 11, 310-315. Go to original source... Go to PubMed...
    13. Huo L.J., Liang C.G., Yu L.Z., Zhong Z.S., Yang Z.M., Fan H.Y., Chen D.Y., Sun Q.Y. (2005): Inducible nitric oxide synthase-derived nitric oxide regulates germinal vesicle breakdown and first polar body emission in the mouse oocyte. Reproduction, 129, 403-409. Go to original source... Go to PubMed...
    14. Hyslop L.A., Carroll M., Nixon V.L., Mcdougall A., Jones K.T. (2001): Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilization. Developmental Biology, 234, 216-230. Go to original source... Go to PubMed...
    15. Im G.S., Samuel M., Lai L., Hao Y., Prather R.S. (2007): Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Molecular Reproduction and Development, 74, 1158-1164. Go to original source... Go to PubMed...
    16. Jílek F., Huttelová R., Petr J., Holubová M., Rozinek J. (2000): Activation of pig oocytes using calcium ionphore: effect of protein synthesis inhibitor cycloheximide. Animal Reproduction Science, 63, 101-111. Go to original source... Go to PubMed...
    17. Jílek F., Huttelová R., Petr J., Holubová M., Rozinek J. (2001): Activation of pig oocytes using calcium ionphore: effect of protein kinase inhibitor 6-dimethyl aminopurine. Reproduction in Domestic Animals, 36, 139-145. Go to original source... Go to PubMed...
    18. Jones K.T. (2005): Mammalian egg activation: from Ca2+ spiking to cell cycle progression. Reproduction, 130, 813-823. Go to original source... Go to PubMed...
    19. Jones K.T., Cruttwell C., Parrington J., Swann K. (1998): A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca 2+ release in sea urchin egg homogenates. FEBS Letters, 437, 297-300. Go to original source... Go to PubMed...
    20. Kim J.H., Lee S.H., Kim S., Jeong Y.W., Koo O.J., Hashem M.D.A., Park S.M., Lee E.G., Hossein M.S., Kang S.K., Lee B.C., Hwang W.S. (2006): Embryotrophic effects of ethylenediaminetetraacetic acid and hemoglobin on in vitro porcine embryos development. Theriogenology, 66, 449-455. Go to original source... Go to PubMed...
    21. Kline D., Kline J.T. (1992): Repetitive calcium transients and the role of calcium in exocytosis and cell-cycle activation in the mouse egg. Developmental Biology, 149, 80-89. Go to original source... Go to PubMed...
    22. Konig S., Muller H., Tesfaye D., Jennen D., Tholen E., Gilles M., Rings F., Montag M., Schellander K., Hoelker M. (2006): The bovine phospholipase C zeta protein as the physiological sperm-derived trigger of Ca2+ oscillations at bovine oocyte activation. Reproduction in Domestic Animals, 41, 333-334.
    23. Krsmanovic L.Z., Stojilkovic S.S., Catt K.J. (1996): Pulsatile gonadotropin-releasing hormone release and its regulation. Trends in Endocrinology and Metabolism, 7, 56-59. Go to original source... Go to PubMed...
    24. Kuo R.K., Baxter G.T., Thompson S.H., Stricker S.A., Patton C., Bonaventura J., Epel D. (2000): NO is necessary and sufficient for egg activation at fertilization. Nature, 406, 633-636. Go to original source... Go to PubMed...
    25. Leckie C., Empson R., Becchetti A., Thomas J., Galione A., Whitaker M. (2003): The NO pathway acts late during the fertilization response in sea urchin eggs. Journal of Biological Chemistry, 278, 12247-12254. Go to original source... Go to PubMed...
    26. Lorca T., Cruzalegui F.H., Fesquet D., Cavadore J.C., Mery J., Means A., Doree M. (1993): Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs. Nature, 366, 270-273. Go to original source... Go to PubMed...
    27. Malcuit C., Knott J.G., He C., Wainwright T., Parys J.B., Robl J.M., Fissore R.A. (2005): Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor downregulated bovine eggs. Biology of Reproduction, 73, 2-13. Go to original source... Go to PubMed...
    28. Malcuit C., Kurokawa M., Fissore R.A. (2006a): Calcium oscillations and mammalian egg activation. Journal of Cellular Physiology, 206, 565-573. Go to original source... Go to PubMed...
    29. Malcuit C., Maserati M., Takahashi Y., Page R., Fissore RA. (2006b): Intracytoplasmic sperm injection in the bovine induces abnormal (Ca2+)i response and oocyte activation. Reproduction, Fertility and Development, 18, 1-13. Go to original source... Go to PubMed...
    30. Medlicott N.J., Tucker I.G. (1999): Pulsatile release from subcutaneous implants. Advanced Drug Delivery Reviews, 38, 139-149. Go to original source... Go to PubMed...
    31. Mori H., Mizobe Y., Inoue S., Uenohara A., Takeda M., Yoshida M., Miyoshi K. (2008): Effects of cycloheximide on parthenogenetic development of pig oocytes activated by ultrasound treatment. Journal of Reproduction and Development, 54, 364-369. Go to original source... Go to PubMed...
    32. Morozumi K., Shikano T., Miyazaki S., Yanagimachi R. (2006): Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development. Proceedings of the National Academy of Sciences of United States of America, 103, 17661-17666. Go to original source... Go to PubMed...
    33. Nussbaum D.J., Prather R.S. (1995): Differential effects of protein synthesis inhibitors on porcine oocyte activation. Molecular Reproduction and Development, 41, 70-75. Go to original source... Go to PubMed...
    34. Ozil J.P., Markoulaki S., Toth S., Matson S., Banrezes B., Knott J.G., Schultz R.M., Huneau D., Ducibella T. (2006a): Egg activation events are regulated by the duration of a sustained (Ca2+)(Cyt) signal in the mouse. Developmantal Biology, 282, 39-54. Go to original source... Go to PubMed...
    35. Ozil J.P., Banrezes B., Toth S., Pan H., Schultz R.M. (2006b): Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term. Developmental Biology, 300, 534-544. Go to original source... Go to PubMed...
    36. Parrington J., Jones M.L., Tunwell R., Devader C., Katan M., Swann K. (2002): Phospholipase C isoforms in mammalian spermatozoa: potential components of the sperm factor that causes Ca2+ release in eggs. Reproduction, 123, 31-39. Go to original source... Go to PubMed...
    37. Petr J., Grocholová R., Rozinek J., Jílek F. (1996): Activation of in vitro matured pig oocytes by combined treatment of ethanol and cycloheximide. Theriogenology, 1473-1478. Go to original source...
    38. Petr J., Rajmon R., Rozinek J., Sedmiková M., Ješeta M., Chmelíková E., Švestková D., Jílek F. (2005a): Activation of pig oocytes using nitric oxide donors. Molecular Reproduction and Development, 71, 115-122. Go to original source... Go to PubMed...
    39. Petr J., Rajmon R., Lanská V., Sedmíková M., Jílek F. (2005b): Nitric oxide-dependent activation of pig oocytes: role of calcium. Molecular and Cellular Endocrinology, 242, 16-22. Go to original source... Go to PubMed...
    40. Petr J., Rajmon R., Chmelíková E., Tománek M., Lanská V., Pribanová M., Jílek F. (2006): Nitric-oxide-dependent activation of pig oocytes: the role of the cGMPsignalling pathway. Zygote, 14, 9-16. Go to original source... Go to PubMed...
    41. Petr J., Chmelíková E., Tumová L., Ješeta M. (2007a): The role of nitric oxide in parthenogenetic activation of pig oocytes: A review.. Czech Journal of Animal Science, 52, 363-377. Go to original source...
    42. Petr J., Chmelíková E., Dorflerová A., Ješeta M., Kuthanová Z. (2007b): Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor. Czech Journal of Animal Science, 52, 415-422. Go to original source...
    43. Petr J., Chmelíková E., Krejčová T., Řehák D., Novotná B., Jílek F. (2009): Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor. Reproduction in Domestic Animals, (in press - 10.1111/j.1439-0531.2008.01275.x) Go to original source... Go to PubMed...
    44. Petters R.M., Wells K. (1993): Culture of pig embryos. Journal of Reproduction and Fertility, 48, 61-73.
    45. Porksen N. (2002): The in vivo regulation of pulsatile insulin secretion. Diabetologia, 45, 3-20. Go to original source... Go to PubMed...
    46. Pressice G.A., Yang X.Z. (1994): Parthenogenetic development of bovine oocytes matured in vitro for 24 hr and activated by ethanol and cycloheximide. Molecular Reproduction and Development, 38, 380-385. Go to original source... Go to PubMed...
    47. Rogers N.T., Halet G., Piao Y., Carroll J., Ko M.S.H., Swann K. (2006): The absence of a Ca2+ signal during mouse egg activation can affect parthenogenetic preimplantation development, gene expression patterns, and blastocyst quality. Reproduction, 132, 45-57. Go to original source... Go to PubMed...
    48. Salerno J. (2008): Neuronal nitric oxide synthase: Prototype for pulsed enzymology. FEBS Letters, 582, 1395-1399. Go to original source... Go to PubMed...
    49. Soloy E., Kanka J., Viuff D., Smith S.D., Callesen H., Greve T. (1997): Time course of pronuclear deoxyribonucleic acid synthesis in parthenogenetically activated bovine oocytes. Biology of Reproduction, 57, 27-35. Go to original source... Go to PubMed...
    50. Sparkman L., Boggaram V. (2004): Nitric oxide increases Il-8 gene transcription and mRNA stability to enhance Il-8 gene expression in lung epithelial cells. American Journal of Physiolofy-Lung Cellular and Molecular Physiology, 287, L764-L773. Go to original source... Go to PubMed...
    51. Stefanelli C., Pignatti C., Tantini B., Stanic I., Bonavita F., Muscari C., Guarnieri C., Clo C., Caldarera C.M. (1999): Nitric oxide can function as either a killer molecule or an antiapoptotic effector in cardiomyocytes. Biochimica and Biophysica Acta -Molecular Cell Research, 1450, 406-413. Go to original source... Go to PubMed...
    52. Swann K., Ozil J.P. (1994): Dynamics of the calcium signal that triggers mammalian egg activation. International Review of Cytology, 152, 183-222. Go to original source... Go to PubMed...
    53. Swann K., Lai F.A. (1997): A novel signalling mechanism for generating Ca2+ oscillations at fertilization in mammals. Bioessays, 19, 371-378. Go to original source... Go to PubMed...
    54. Terasawa E. (2001): Luteinizing hormone-releasing hormone (LHRH) neurons: mechanism of pulsatile LHRH release. Vitamins and Hormones, 63, 91-129. Go to original source... Go to PubMed...
    55. Toth S., Huneau D., Banrezes B., Ozil J.P. (2006): Egg activation is the result of calcium signal summation in the mouse. Reproduction, 131, 27-34. Go to original source... Go to PubMed...
    56. Tranguch S., Steuerwald N., Huet-Hudson Y.A. (2003): Nitric oxide synthase production and nitric oxide regulation of preimplantation embryo development. Biology of Reproduction, 68, 1538-1544. Go to original source... Go to PubMed...
    57. Whitaker M. (2006): Calcium at fertilization and in early development. Physiological Review, 86, 25-88. Go to original source... Go to PubMed...
    58. Xu Z., Kopf G.S., Schultz R.M. (1994): Involvement of inositol 1,4,5-trisphosphate-mediated Ca2+ release in early and late events of mouse egg activation. Development, 120, 1851-1859. Go to original source... Go to PubMed...
    59. Xu L., Eu J.P., Meissner G., Stamler J.S (1998): Activation of the cardiac calcium release channel (ryanodine receptor) by poly-S-nitrosylation. Science, 279, 234-237. Go to original source... Go to PubMed...
    60. Yanagimachi R. (1988): Mammalian fertilization. In: The Physiology of Reproduction. Knobil E., Neil J. (eds.): Raven Press: New York, USA, 135-185.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content