Czech Journal of Animal Science, 2021 (vol. 66), issue 3
Detection of copy number variation of alpha amylase genes in domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa)Original Paper
Tsukasa Yoshidomi, Kensuke Hirose, Takeshi Kuge, Yukio Okada, Yaetsu Kurosawa, Tatsuya Takizawa, Kazuaki Tanaka
Czech J. Anim. Sci., 2021, 66(3):73-77 | DOI: 10.17221/61/2020-CJAS
Copy numbers of alpha amylase genes (AMY), which encode starch-digesting enzymes, are markedly increased in modern humans and domesticated dogs as an adaptive evolutionary mechanism in response to increased consumption of starch-rich foods acquired either by farming or domestication. In this study, we surveyed total AMY gene copy numbers in 150 domestic pigs (50 pigs of Berkshire breed, 50 of Landrace breed, and 50 of Large White breed) and 51 wild boars (30 Sus scrofa leucomystax and 21 S. s. riukiuanus) to identify whether the gene copy number has changed during the domestication of pigs. The relative copy number...
The analysis of pig carcass classification in SlovakiaOriginal Paper
Ján Tomka, Peter Demo, Martina Gondeková, Zuzana Salagová
Czech J. Anim. Sci., 2021, 66(3):78-86 | DOI: 10.17221/231/2020-CJAS
In the last decade, the pig sector in Slovakia has been changing continuously, especially in regard to the proportion of pigs coming from foreign breeding programs. These changes lead to changes in carcass characteristics and to a change in the distribution of carcasses within the classes of the Union scale for the classification of pig carcasses. Therefore, this study was undertaken to analyse the actual state of pig carcass classification in the Slovak Republic and to investigate the needs of updating the equations for the classification methods and possible steps to improve the quality of pork produced within the country. Results showed that an...
Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cellsOriginal Paper
Shaoyu Wang, Kui Li, Hui Gao, Zepeng Liu, Shuang Shi, Qiang Tan, Zhengguang Wang
Czech J. Anim. Sci., 2021, 66(3):87-96 | DOI: 10.17221/105/2020-CJAS
Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted...
The response of fast-, medium- and slow-growing chickens to a low protein dietOriginal Paper
Darina Chodová, Eva Tůmová, Mohamed Ketta
Czech J. Anim. Sci., 2021, 66(3):97-105 | DOI: 10.17221/260/2020-CJAS
The aim of the present study was to evaluate the effect of two dietary protein levels on performance, carcass characteristics, and meat quality parameters in fast- (Ross 308), medium- (JA757), and slow-growing (ISA Dual) chickens to assess the interaction of the two factors. Each genotype was divided into a control group fed a commercial type of feed mixture and an experimental group fed a low-protein diet (LP). The trial was terminated after a common period of fattening of each genotype, and 20 chickens per group (sex ratio 1 : 1) were selected for the carcass and meat analysis. The results indicated that the LP diet decreased growth (P < 0.001)...