Czech Journal of Animal Science, 2023 (vol. 68), issue 9
Changes in the behaviour of Czech Fleckvieh dairy cows during the day under optimal ambient temperature conditions in the barnOriginal Paper
Milan Veèeøa, Stanislav Navrátil, Tomá¹ Kopec, Daniel Falta, Gustav Chládek, Francois Stefanus Lategan
Czech J. Anim. Sci., 2023, 68(9):365-371 | DOI: 10.17221/23/2023-CJAS 
In the experimental section of the barn (which made up 25% of the barn), 103 cubicles in free stall barn were selected in three rows, housing 98 dairy cows of Czech Fleckvieh cattle. Monitoring was done for one calendar year, once a week. From this period, 16 days with temperatures from 0.5–15.0 °C were selected for quantification. Observations were continuously recorded at 10:00 am (one hour after the coming of the last cow from the milking parlour), at hourly intervals until 7:00 pm. Ten observations were carried out every day of the monitoring period. In total, 15 680 individual observations were recorded. The microclimatic parameters (temperature,...
In vitro assessment of the relationships between the digestion of different types of rice straw and bacterial community in the rumenOriginal Paper
Ajmal Wali, Naoki Nishino
Czech J. Anim. Sci., 2023, 68(9):372-382 | DOI: 10.17221/193/2022-CJAS
The aims of this study were to examine bacterial communities in relation to the rumen digestion of rice straw and to understand how concentrate supplements affect gut bacteria involving the digestion of a rice straw-based diet. The substrates were rice straw (RS) alone (experiment 1) and RS with 25% concentrates (barley and kidney beans) (experiment 2). The genomic DNA was collected to determine bacterial diversity by conducting denaturing gradient gel electrophoresis (DGGE). V6–V8 region group-specific (Clostridium and Bacteroides) primers were employed in the analyses. The DGGE band pattern was subjected to cluster analysis to demonstrate...
Evaluation of crystallisation structures of cervical mucus in Zwartbles sheep with previous oestrus synchronisationOriginal Paper
Vojtìch Pe¹an, Zuzana Reèková, Martin Ho¹ek, Radek Filipèík, Katarina Sou¹ková, Tomá¹ Kopec, Martina Pe¹anová Tesaøová
Czech J. Anim. Sci., 2023, 68(9):383-390 | DOI: 10.17221/4/2023-CJAS
This study was conducted to determine the individual types of crystallisation structures of cervical mucus (arborisation phenomenon) in sheep and changes in the types of these structures during oestrus. A total of 80 ewes aged between two and eight years were included in the study. Oestrus synchronisation was performed using intravaginal sponges, and samples of cervical mucus were collected 55–57 h after the removal of intravaginal sponges and subsequently evaluated. The crystallisation structures of the cervical mucus of sheep exhibit the same sequence during oestrus as in cattle. The most frequently occurring types of crystallisation in the...
Gly-Leu instead of Gly promoted the proliferation and protein synthesis of chicken intestinal epithelial cellsOriginal Paper
Jing Liang, Baolong Du, Minyan Wan, Yapeng Zhao, Defu Tang
Czech J. Anim. Sci., 2023, 68(9):391-398 | DOI: 10.17221/213/2022-CJAS
Amino acids have positive regulatory effects on the function of intestinal epithelial cells (IEC), but in the field of animal nutrition, research on the regulatory effects of amino acids on IEC is still in the initial stages. This study aims to explore the effects of Gly, Gly-Gly, and Gly-Leu on IEC proliferation and their possible mechanisms. Chicken small intestinal epithelial cells were separated using the tissue block method, and other miscellaneous cells were removed for digestion and passage culture. The IEC were cultured in the medium containing 20 nmol/l Gly, Gly-Gly and Gly-Leu for 24 h, and the expression of enterokinase and cytokeratin in cells,...