Czech Journal of Animal Science, 2022 (vol. 67), issue 10
Long-term pedigree analysis: An effective tool for managing congenital malformations in cattleOriginal Paper
Jindřich Čítek, Libor Večerek, Soňa Šlosárková, Petr Fleischer, Luboš Vostrý, Hana Vostrá-Vydrová, Daniela Schröffelová, Josef Kučera
Czech J. Anim. Sci., 2022, 67(10):385-393 | DOI: 10.17221/70/2022-CJAS 
Controlling congenital defects is an important aspect of breeding for genetic health; however, whether malformations are caused by genetic or nongenetic factors may not be clear. This study aims to analyse the incidence of aplasia genitalis, atresia ani et recti and hernia cerebralis in the Czech cattle population, analyse the relatedness of affected calves by a relationship matrix, and assess the potential future threat. The sires fathering affected calves were born in the Czech Republic or imported from 1986 to 2001. The cases occurred on farms across the Czech Republic. The pedigree of each case was examined for common...
Impact of different dietary fibre levels on the roughage resistance of the Dahe black pigOriginal Paper
Ye Wang, Chaoying Liu, Jinjin Zhu, Enzhong Li, Mingcheng Wang, Zhichao Shen, Xinfeng Yang, Guojin Lv, Rongfu Guo
Czech J. Anim. Sci., 2022, 67(10):394-406 | DOI: 10.17221/63/2022-CJAS
To investigate the effects and molecular mechanism of different levels of dietary fibre (Chinese milk vetch) on Dahe black and Duroc × (Landrace × Yorkshire) (DLY) pigs, 54 of each type of pig were selected and fed diets that contained 3.5%, 5.5% and 7.5% levels of fibre in the growing (30-60 kg) and fattening stages (60-120 kg). The production performance, serum physicochemical indices, nutrient digestibility, the amylase activity in the small intestine, contents of the muscle crude protein and ether extract, and the levels of transcription of the PRKAG3 and Fsp27 genes were determined. The production performance...
Transformation of a short-term boar semen extender into a long-term boar semen extender by using penicillamineOriginal Paper
Panida Chanapiwat, Shutipen Buranasinsup, Kampon Kaeoket
Czech J. Anim. Sci., 2022, 67(10):407-415 | DOI: 10.17221/130/2022-CJAS
This study aimed to convert a short-term semen extender into a long-term semen extender by adding penicillamine (PEN). Semen samples were collected from six boars, diluted at a concentration 3 × 109 spermatozoa/100 ml, and divided into eight groups as follows: BTS, Merck III®, Androstar® Plus, PEN 0.125 mM, PEN 0.25 mM, PEN 0.5 mM, PEN 1.0 mM, PEN 2.0 mM. All...
Isolation, culture and identification of sheep skeletal muscle satellite cellsOriginal Paper
Sailuo Wan, Qianqian Pan, Jinbo Wei, Cuiyun Zhu, Jing Jing, Shuaiqi Qin, Rongcui Hu, Mengyu Lou, Shuang Li, Yinghui Ling
Czech J. Anim. Sci., 2022, 67(10):416-423 | DOI: 10.17221/52/2022-CJAS
Skeletal muscle satellite cells (SMSCs) play an important role in muscle growth, regeneration and maintenance. This study aimed to isolate SMSCs from sheep, establish a system for isolation, culture and identification of SMSCs in vitro, and provide seed cells for subsequent studies. SMSCs were isolated and purified from newborn 2-day-old healthy sheep by collagenase type I and trypsin two-step digestion and pre-plating method. The results indicated that the isolated and purified SMSCs showed full spindle shape and strong refractive index. The cell growth curve detected by CCK-8 kit was typical "S" type. Immunofluorescence analysis showed that...