Czech Journal of Animal Science, 2025 (vol. 70), issue 3
Analysis of the mare breeding population of Haflinger breed in the Czech RepublicOriginal Paper
Michaela Kuliığáková, Eva Sobotková, Radek Filipèík
Czech J. Anim. Sci., 2025, 70(3):83-92 | DOI: 10.17221/8/2025-CJAS 
The aim of this study was to analyse the conformation traits and body dimensions of Haflinger mares registered in the Czech Republic studbook. The study focused on the effects of age at studbook entry, year of birth, proportion of Arabian genes, and country of origin on various parameters. Data were collected from 825 mares, including scores obtained during the studbook entry evaluation and body measurements such as withers height measured with a stick (WHS), withers height measured with a tape (WHT), chest girth (CG), and cannon bone circumference (CBC). Based on these data, the bone index (BI) and body massiveness index (MI) were calculated. Statistical...
Association between conventional semen variables and sperm freezability in ramsOriginal Paper
Aizhan Makhanbetova, Filipp Georgijeviè Savvulidi, Martin Ptáèek, Lucie Langerová, Beybit Kulataev, Nurlan Malmakov
Czech J. Anim. Sci., 2025, 70(3):93-101 | DOI: 10.17221/185/2024-CJAS
Assessments of routine ejaculated samples were used to evaluate the conventional semen variables (sperm motility, semen volume, sperm concentration) as initial decision-making criteria to freeze or not to freeze. However, the association of these attributes to the cryotolerance of ram sperm has not been adequately studied yet. The aim of this study was therefore to analyse if the semen volume, sperm motility and concentration can be used to predict post-thaw ram sperm motility. Additionally, in the present study we tested if the order of ejaculate sampling (first versus second, within a single collection session) and the breed of rams influenced...
LGALS3 promotes the proliferation of Liaoning Cashmere goat skin fibroblasts by regulating the expression of PIEZO1Original Paper
Mei Jin, Weiyu Fan, Jinxu Xu
Czech J. Anim. Sci., 2025, 70(3):102-112 | DOI: 10.17221/72/2024-CJAS
Long non-coding RNA (lncRNA) plays a role in the reconstruction of hair follicles and in the formation and growth of cashmere fibres. One cashmere growth-associated lncRNA was successfully screened out at the early stage in the laboratory, which was named LncRNA MTC. In this study, the binding of galectin 3 (LGALS3) to LncRNA MTC was examined through RNA immunoprecipitation (RIP) in skin fibroblasts of Liaoning Cashmere goats. The pLenti-LGALS3-His-IRES-EGFP lentiviral overexpression vector was built, and the result of CCK8 indicated that the overexpression of LGALS3 significantly enhanced the viability of skin fibroblasts (P < 0.05)....
Whole-genome resequencing data reveal the genetic diversity of local chickens in southern Zhejiang and surrounding areas in ChinaOriginal Paper
Yawen Zhang, Bo Zhang, Ziwei Li, Fengxiang Hou, Yan Zhao, Junjie Jin, Bin Song, Wenjie Gu
Czech J. Anim. Sci., 2025, 70(3):113-120 | DOI: 10.17221/186/2024-CJAS
China’s local chicken breeds, especially those in the Zhejiang Province, are rich in genetic diversity owing to environmental heterogeneity and complex ecosystems. Distinctive local breeds have emerged through long-term natural selection and domestication. We investigated the genetic diversity and population structure of local chickens in southern Zhejiang and surrounding areas using whole-genome resequencing of 129 chickens from seven populations. A total of 1.8 terabytes of raw data was obtained, and 4 802 728 single nucleotide polymorphisms were detected. The Xianju chicken population exhibited the highest genetic diversity, while Yandang...