Czech Journal of Animal Science, 2026 (vol. 71), issue 7
The mechanism of N-carbamylglutamate promoting arginine synthesis and its application in pig production: A reviewReview
Pei Mao, MengMeng Gao, Naizhi Hu, XiaoLi Zhang, ChengGang Yan, ChengSheng Song, WenFeng Ma
Czech J. Anim. Sci., 2026, 71(7):299-307 | DOI: 10.17221/87/2025-CJAS 
Arginine (Arg) is an essential amino acid that can improve the reproductive capacity, intestinal development, and growth performance of pigs. N-carbamylglutamate (NCG) and N-acetylglutamate (NAG) can activate carbamyl phosphate synthetase I and the Δ1 pyrrolin 5 carboxylate synthetase in mitochondria, promoting the synthesis of citrulline from glutamine and proline, citrulline is the main precursors for the synthesis of Arg, and subsequently increasing the endogenous synthesis of Arg to produce nitric oxide (NO) and polyamines. Compared with Arg, NCG has a longer half-life and does not inhibit the absorption of basic amino...
Rumen-protected gambir catechins encapsulated using CaO-PFAD: Their effects on rumen fermentation, nutrient digestibility, and methane production in vitroOriginal Paper
Roni Pazla, Asmuddin Natsir, Dewi Febrina, Mardiati Zain, Arief Arief, Antonius Antonius, Zaitul Ikhlas, Yolani Utami, Ezi Masdia Putri, Gusri Yanti, Yanuar Achadri, Sharli Asmairicen, Ida Andriani
Czech J. Anim. Sci., 2026, 71(7):308-318 | DOI: 10.17221/68/2026-CJAS
The objective of this study was to evaluate the impact of rumen-protected gambir catechins encapsulated using calcium oxide (CaO) and palm fatty acid distillate (PFAD) on rumen fermentation profiles, microbial activity, nutrient digestibility, and methane production in vitro. A randomised block design was used, consisting of four treatments and five replicates. The treatments were assigned based on different levels of gambir catechin-CaO-PFAD encapsulate supplementation, namely 0% (A), 5% (B), 10% (C), and 15% (D) in the diet. The results indicated that the treatments significantly affected NH3 concentration, partial VFA profiles,...
Improved cryotolerance of bovine blastocysts by 1α,25-dihydroxyvitamin D3 supplementation during in vitro culture and post-thaw recoveryOriginal Paper
HakKyo Lee, Do-Hyun Kim, DaeJin Kwon
Czech J. Anim. Sci., 2026, 71(7):319-329 | DOI: 10.17221/61/2026-CJAS
In vitro embryo production (IVP) is a valuable technique for advancing cattle breeding and reproductive biotechnology. Nevertheless, embryos produced in vitro often display reduced developmental competence and cryotolerance compared with their in vivo counterparts. Vitamin D is known to regulate many cellular functions, including growth and antioxidant defence. This study evaluated the impact of vitamin D supplementation during in vitro culture on bovine embryo development and post-thaw recovery. Oocytes were matured and fertilised in vitro and presumptive zygotes were cultured with 0 (control), 25, or 50 ng/ml...
The impact of folic acid on genome-wide DNA methylation profiles in the liver of broilers
Yuting Pang, Yujie Zhang, Yunheng Liu, Xiuling Li, Jinyi Xing
Czech J. Anim. Sci., 2026, 71(7):330-341 | DOI: 10.17221/102/2025-CJAS
Folic acid (FA) plays an important role as a cofactor and coenzyme in animal growth and development, and in the regulation of gene expression and methylation. However, the epigenetic mechanism by which dietary FA supplementation regulates lipid metabolism in broilers remains largely unknown. Therefore, this study aimed to investigate the effects of FA on genome-wide DNA methylation profiles in broiler liver and to identify differentially methylated genes (DMGs) and pathways associated with lipid metabolism. Reduced representation bisulphite sequencing (RRBS) was performed to analyse genome-wide DNA methylation profiles in liver tissues of broilers...