Czech Journal of Animal Science, 2021 (vol. 66), issue 10
Biological response of piglets challenged with Escherichia coli F4 (K88) when fed diets containing intestinal alkaline phosphataseOriginal Paper
Jansller Luiz Genova, Paulo Evaristo Rupolo, Antonio Diego Brandão Melo, Liliana Bury de Azevedo dos Santos, Geraldyne Nunes Wendt, Keila Abadia Barbosa, Silvana Teixeira Carvalho, Newton Tavares Escocard de Oliveira, Leandro Batista Costa, Paulo Levi de Oliveira Carvalho
Czech J. Anim. Sci., 2021, 66(10):391-402 | DOI: 10.17221/82/2021-CJAS 
The aim of the study was to investigate the effect of intestinal alkaline phosphatase (IAP) added to diets on growth performance, diarrhoea incidence (DI), blood metabolites, relative organ weight, and intestinal morphometry of weaned piglets challenged with enterotoxigenic Escherichia coli F4 (K88). A total of 64 crossbred entire male piglets (25-day-old and 7.16 ± 0.28 kg body weight) were allocated into four treatments: control diet (CD-), CD- + antimicrobial growth promoter (AGP), CD- + 15 mg IAP/kg of diet and CD- + 30 mg IAP/kg of diet, with eight replications. At 15 days, all piglets...
The role of quercetin in primary culture of ovine spermatogonial stem cellsOriginal Paper
Fatemeh Emamdoust, Mohammad Zandi, Mehdi Aminafshar, Mohammad Reza Sanjabi
Czech J. Anim. Sci., 2021, 66(10):403-411 | DOI: 10.17221/14/2021-CJAS
The aim of the present study was to examine the effect of quercetin on the survival and primary culture of ovine spermatogonial stem cells (SSCs). The two-time enzymatic digestion process was employed to obtain SSCs from lamb testes. In the next step, the use of filtration and differential plating methods caused an increase in the number of SSCs in the cell suspension resulting from enzymatic and mechanical digestions. Mitomycin-C-treated Sertoli cells were used to prepare the feeder layer. The stem cells were then cultured on the Sertoli cell feeder layer. The identification of the colonies was done through alkaline phosphatase staining methods and...
Prenatal effects of red and blue light on physiological and behavioural parameters of broiler chickensOriginal Paper
Angelika Drozdová, Zuzana Kaňková, Boris Bilčík, Michal Zeman
Czech J. Anim. Sci., 2021, 66(10):412-419 | DOI: 10.17221/80/2021-CJAS
Light during incubation can influence embryonic and postembryonic development of chickens, but the underlying mechanisms are poorly understood. Previous studies have demonstrated that red and blue lights during incubation had opposite effects on the development of embryonic melatonin biosynthesis; red light results in the highest and blue light in the lowest amplitude of the daily rhythm. Therefore, in this study, we investigated if exposure to monochromatic red (632 nm) and blue (463 nm) light during incubation can differently influence growth, selected biochemical (glucose, cholesterol, triacylglycerols) and endocrine (corticosterone and thyroid...
Comparison of starch digestibility methods for extruded wheat grains (Triticum aestivum L.)Original Paper
Dana Homolková, Vladimír Plachý, Anna Maňourová, Michal Kaválek, Václav Dvořáček, Boris Hučko, Milan Marounek, Ivo Doskočil
Czech J. Anim. Sci., 2021, 66(10):420-427 | DOI: 10.17221/56/2021-CJAS
This study compared different methods of determining starch digestibility (in vivo vs in vitro) in wheat grains and evaluated the influence of extrusion on digestibility. In vivo starch digestibility was determined in broiler chickens by calculating the residual starch content in their ilea and the digestibility using a chromium oxide indicator. In vitro digestibility was examined using pepsin and pancreatin. During in vivo testing, the highest digestibility coefficient (DC) was achieved by the Bonanza variety in its extruded form (91.19 ± 0.40%). In contrast, the lowest DC was achieved by the Tobak...
Effect of density and mixed culture of largemouth bass (Micropterus salmoides) and pikeperch (Sander lucioperca) on growth, survival and feed conversion rate in intensive cultureOriginal Paper
Tomáš Pěnka, Oleksandr Malinovskyi, Jiří Křišťan, Aiman Imentai, Tomáš Policar
Czech J. Anim. Sci., 2021, 66(10):428-440 | DOI: 10.17221/59/2021-CJAS
In this study, two experiments were performed with the aim to optimize intensive aquaculture of largemouth bass (Micropterus salmoides). In the first 140-day experiment the effect of the initial fish density was assessed at three levels: low density (LD) 23 kg/m3, medium density (MD) 35 kg/m3 and high density (HD) 46 kg/m3. All three densities provided the same final Fulton's condition coefficient (FC = 1.24-1.28), specific growth rate (SGR = 0.22-0.24%/day) and survival rate (97-100%). No cannibalism was observed at all tested densities. Feed conversion ratio (FCR = 1.39 ± 0.21 g/g) was the lowest for...