Czech Journal of Animal Science, 2009 (vol. 54), issue 7
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
T. Krejčová, J. Petr, M. Krejčová, K. Kheilová
Czech J. Anim. Sci., 2009, 54(7):293-306 | DOI: 10.17221/1724-CJAS 
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to...
Differentiation of porcine wild-type lactobacilli strains, with ERIC-PCR and PFGE band patterns included in polyphasic taxonomy
E. Bolado-Martínez, E. Acedo-Félix
Czech J. Anim. Sci., 2009, 54(7):307-314 | DOI: 10.17221/1722-CJAS
Probiotic administration is an alternative to antibiotic supplementation in pig management. However, potential probiotic microorganisms included in foods must be well defined and correctly named, on the basis of a valid taxonomic system. The aim of the present study was to analyze band patterns generated by the polymerase chain reaction (PCR) amplification of enterobacterial repetitive intergenic consensus sequences (ERIC-PCR), obtained from porcine wild-type Lactobacillus strains, as a rapid alternative for genotypic characterization in polyphasic taxonomy. In the present study 36 porcine wild-type Lactobacillus strains were analyzed...
Effects of grass species on ruminal degradability of silages and prediction of dry matter effective degradability
F. Jančík, V. Koukolová, P. Kubelková, B. Čermák
Czech J. Anim. Sci., 2009, 54(7):315-323 | DOI: 10.17221/1725-CJAS
Forty samples of grass silages, made from the five most widely used grass species in ruminant nutrition (Dactylis glomerata L.,Phleum pratense L., Lolium perenne L., Festuca arundinacea L. and hybrid Felina) were tested in the present study. These grass species conserved by an ensiling process were compared among each other according to dry matter (DM) rumen degradability parameters (a = portion of DM solubilized at initiation of incubation, b = fraction of DM potentially degradable in the rumen, c = rate constant of disappearance of fraction b and EDDM = effective degradability of DM, estimated...
Chemical composition of bone tissue in broiler chickens intended for slaughter
P. Suchý, E. Straková, I. Herzig, L. Steinhauser, G. Králik, D. Zapletal
Czech J. Anim. Sci., 2009, 54(7):324-330 | DOI: 10.17221/1726-CJAS
Selected parameters characterizing the chemical composition of bones were determined in 60 male and 60 female hybrid Ross 308 chickens aged 40 days at the end of the fattening period. The chickens received a complete feed mixture intended for pre-feeding (starter) in a period from the start of the experiment until Day 10, followed by the feed mixture intended for fattening until Day 30 (grower), and the feed mixture for the third phase of fattening, i.e. until Day 40 (finisher). The feed mixtures and drinking water were provided ad libitum. At the end of the experiment, chickens were slaughtered and the femur and the tibiotarsus from the right...
Response of larval and juvenile rudd Scardinius erythrophthalmus (L.) to different diets under controlled conditions 331
J. Wolnicki, J. Sikorska, R. Kamiński
Czech J. Anim. Sci., 2009, 54(7):331-337 | DOI: 10.17221/1723-CJAS
The growth and survival of rudd Scardinius erythrophthalmus (L.) were evaluated in a laboratory at 25°C. In 20-day Experiment 1, first-feeding larvae at the age of 4 days post-hatch (initially: TL = 5.7 mm, BW = 0.9 mg) were fed live Artemia nauplii or commercial dry feed (Aller Futura Larvae, AFL) or combinations of both. Even the longest period of initial feeding of nauplii (6 days) was insufficient to obtain satisfactory larval growth after weaning to AFL (TL = 12.4 mm and BW = 17.7 mg vs. TL = 18.9 mm and BW = 68.5 mg for the nauplii-fed fish, significant differences). Nauplii-fed older larvae (24 days post-hatch) were then used in...