Czech Journal of Animal Science, 2021 (vol. 66), issue 1
The assignment success for 22 horse breeds registered in the Czech Republic: The machine learning perspectiveOriginal Paper
Lenka Štohlová Putnová, Radek Štohl
Czech J. Anim. Sci., 2021, 66(1):1-12 | DOI: 10.17221/120/2020-CJAS 
The paper demonstrates the dependability of assignment testing in the identification of an appropriate breed to monitor comprehensive genetic information from molecular markers to analyse the collection of real population data covering 22 horse breeds registered in the Czech Republic, including native breeds and genetic resources. If 17 microsatellites are used, the mean number of alleles per locus corresponds to 10.4. The count of alleles at the individual loci ranges between five (HTG07) and 17 (ASB17). The loci ASB02, ASB23, HMS03, HTG10, and VHL20 exhibit the highest gene diversity...
Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidationOriginal Paper
Yotsapon Yangngam, Suttiwat Chapanya, Thevin Vongpralub, Wuttigrai Boonkum, Vibuntita Chankitisakul
Czech J. Anim. Sci., 2021, 66(1):13-20 | DOI: 10.17221/104/2020-CJAS
Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde - MDA test) were analysed....
l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acidOriginal Paper
Panida Chanapiwat, Kampon Kaeoket
Czech J. Anim. Sci., 2021, 66(1):21-28 | DOI: 10.17221/199/2020-CJAS
The present study aimed to develop a long-term fresh boar semen extender by adding l-cysteine and docosahexaenoic acid (DHA) into Beltsville thawing solution (BTS)-based extender. Semen samples were collected from six boars, diluted at a concentration of 3 × 109 spermatozoa/100 ml and allocated into 10 groups as follows: BTS, Merck III®, Androstar® Plus, ModenaTM, DHA 1.5 mg/ml, 3 mg/ml, and 6.25 mg/ml, l-cysteine 2.5 mM, 5.0 mM, and 10 mM. All extended semen samples were stored at 18 °C and were evaluated for progressive motility, viability, acrosome integrity, pH, and osmolality on days 0, 1,...
Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler dietsOriginal Paper
Júlia Marixara Sousa da Silva, Nayanne Rodrigues de Oliveira, Alison Batista Vieira Silva Gouveia, Rodolfo Alves Vieira, Ronan Omar Fernandes dos Santos, Cibele Silva Minafra, Fabiana Ramos dos Santos
Czech J. Anim. Sci., 2021, 66(1):29-37 | DOI: 10.17221/134/2020-CJAS
Enzymes benefit digestion and absorption of the ingredients and their addition to an animal-origin meal (AOM) can improve its nutritional quality. This research aimed to evaluate the effect of protease on nutrient digestibility, amino acids, and metabolism of AOM energy for broilers. Four hundred and eighty broiler chickens were distributed in a completely randomized design (4 × 2 factorial scheme), eight treatments, six replicates containing 10 birds/replicate. Treatments consisted of poultry viscera meal, swine viscera meal (SVM), bovine meat and bone meal, and basal diet; with and without protease addition. Two tests were performed. In the first...