Czech J. Anim. Sci., 2021, 66(1):13-20 | DOI: 10.17221/104/2020-CJAS

Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidationOriginal Paper

Yotsapon Yangngam1, Suttiwat Chapanya2, Thevin Vongpralub1, Wuttigrai Boonkum1,3, Vibuntita Chankitisakul ORCID...*,1,3
1 Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
2 Lumphayaklang Livestock Semen Production Center, Lopburi, Thailand
3 Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand

Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde - MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

Keywords: antioxidant; cattle; frozen semen; ROS; semen cryopreservation

Published: January 29, 2021  Show citation

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Yangngam Y, Chapanya S, Vongpralub T, Boonkum W, Chankitisakul V. Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation. Czech J. Anim. Sci. 2021;66(1):13-20. doi: 10.17221/104/2020-CJAS.
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    References

    1. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health. 2014 Apr;32(1):1-17. Go to original source... Go to PubMed...
    2. Aghaz F, Khazaei M, Vaisi-Raygani A, Bakhtiyari M. Cryoprotective effect of sericin supplementation in freezing and thawing media on the outcome of cryopreservation in human sperm. Aging Male. 2018 Nov 19: [8 p.] . Go to original source... Go to PubMed...
    3. Atessahin A, Bucak MN, Tuncer PB, Kizil M. Effects of antioxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rumin Res. 2008 Jun;77(1):38-44. Go to original source...
    4. Awda BJ, Mackenzie-Bell M, Buhr MM. Reactive oxygen species and boar sperm function. Biol Reprod. 2009 Sep;81(3):553-61. Go to original source... Go to PubMed...
    5. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev. 2000 Mar;55(3):282-8. Go to original source...
    6. Blesbois E, Grasseau I, Seigneurin F. Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction. 2005 Mar;129(3):371-8. Go to original source... Go to PubMed...
    7. Budai C, Egerszegi I, Olah J, Javor A, Kovacs A. The protective effect of antioxidants on liquid and frozen stored ram semen. Sci Pap Anim Sci Biotechnol. 2014 May 31; 47:46-52.
    8. Bussalleu E, Pinart E, Yeste M, Briz M, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Casas I, Bonet S. Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa. Microsc Res Tech. 2005 Dec 1;68(5):277-83. Go to original source... Go to PubMed...
    9. Cerolini S, Zaniboni L, Maldjian A, Gliozzi T. Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology. 2006 Sep 1;66(4):877-86. Go to original source... Go to PubMed...
    10. Chanapiwat P, Kaeoket K, Tummaruk P. Effects of DHAenriched hen egg yolk and l-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl. 2009 Sep;11(5):600-8. Go to original source... Go to PubMed...
    11. Do L, Namula Z, Luu V, Sato Y, Taniguchi M, Isobe T, Kikuchi K, Otoi T. Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes. Reprod Domest Anim. 2014 Apr;48(2):17-20. Go to original source... Go to PubMed...
    12. Fair S, Doyle DN, Diskin MG, Hennessy AA, Kenny DA. The effect of dietary n-3 polyunsaturated fatty acids supplementation of rams on semen quality and subsequent quality of liquid stored semen. Theriogenology. 2014 Jan 15;81(2):210-9. Go to original source... Go to PubMed...
    13. Isobe T, Ikebata Y, Onitsuka T, Wittayarat M, Sato Y, Taniguchi M, Otoi T. Effect of sericin on preimplantation development of bovine embryos cultured individually. Theriogenology. 2012 Sep 1;78(4):747-52. Go to original source... Go to PubMed...
    14. Isobe T, Ikebata Y, Onitsuka T, Do LTK, Sato Y, Taniguchi M, Otoi T. Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. Cryobiology. 2013 Oct;67(2):184-7. Go to original source... Go to PubMed...
    15. Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem. 1998 Jan; 62(1):145-7. Go to original source... Go to PubMed...
    16. Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol. 2009;16(5):449-57. Go to original source... Go to PubMed...
    17. Khan RU. Antioxidants and poultry semen quality. Worlds Poult Sci J. 2011 Jun;67(2):297-308. Go to original source...
    18. Kumar P, Kumar D, Sikka P, Singh P. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim Reprod Sci. 2015 Jan;152:26-31. Go to original source... Go to PubMed...
    19. Lange-Consiglio A, Meucci A, Cremonesi F. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. 2013 Mar;3(1),21-35. Go to original source... Go to PubMed...
    20. Losano JDA, Angrimani DSR, Rui BR, Bicudo LC, Dalmazzo A, Silva BCS, Viana CHC, Mendes CM, Assumpcao MEOA, Barnabee VH, Nichi M. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote. 2018 Jun;26(3):199-206. Go to original source... Go to PubMed...
    21. Lv C, Larbi A, Wu G, Hong Q, Quan G. Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol. Anim Reprod Sci. 2019 Sep;208:106-27. Go to original source... Go to PubMed...
    22. Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (Delta;ψm) in apoptosis; An update. Apoptosis. 2003 Mar;8(2):115-28. Go to original source... Go to PubMed...
    23. Mandal R, Badyakar D, Chakrabarty J. Role of membrane lipid fatty acids in sperm cryopreservation. Adv Androl. 2014;2014: [9 p.]. Go to original source...
    24. Muino R, Fernandez M, Pena AI. Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007 Jun;42(3):305-11. Go to original source... Go to PubMed...
    25. Quintero-Moreno A, Rigau T, Rodriguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004 Feb;61(4):673-90. Go to original source... Go to PubMed...
    26. Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed Res Int. 2014 Jan;2014: [19 p.]. Go to original source... Go to PubMed...
    27. Ratchamak R, Vongpralub T, Boonkum W, Chankitisakul V. Cryopreservation and quality assessment of boar semen collected from bulk samples. Vet Med-Czech. 2019 May 28;64(5):209-16. Go to original source...
    28. Ratchamak R, Ratsiri T, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J. 2020 Jan-Dec;91(1):e13428. Go to original source... Go to PubMed...
    29. Reddy VS, Yadav B, Yadav CL, Anand M, Swain DK, Kumar D, Kritania D, Madan AK, Kumar J, Yadav S. Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat. Cryobiology. 2018 Oct;84:33-9. Go to original source... Go to PubMed...
    30. Sasaki M, Kato Y, Yamada H, Terada S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol Appl Biochem. 2005 Oct;42(2):183-8. Go to original source... Go to PubMed...
    31. Tao W, Li M, Xie R. Preparation and structure of porous silk sericin materials. Macromol Mater Eng. 2005 Mar; 290(3):188-94. Go to original source...
    32. Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019 Aug 27;6: [15 p.]. Go to original source... Go to PubMed...
    33. Yasmin C, Otoi T, Setiadi MA, Karja NWK. Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin. Acta Vet Hung. 2015 Mar 1;63(1):110-7. Go to original source... Go to PubMed...
    34. Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology. 2016 Jan 1;85(1):47-64. Go to original source... Go to PubMed...
    35. Wu JH, Wang Z, Xu SY. Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chem. 2007 Oct; 103(4): 1255-62. Go to original source...

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