Czech Journal of Animal Science, 2019 (vol. 64), issue 6
Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigsOriginal Paper
Wen-Kui Sun, Chi Cheng, Rui Liu, Yi-Hui Chen, Kai Zeng, Xiao-Hui Chen, Yi-Ren Gu, Jiang-Ling Li, Xue-Bin Lv, Rong Gao
Czech J. Anim. Sci., 2019, 64(6):239-247 | DOI: 10.17221/40/2018-CJAS 
The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin...
Maternal variability of Croatian Spotted goat (Capra hircus)Original Paper
Ivana Drzaic, Ino Curik, Dinko Novosel, Vlatka Cubric-Curik
Czech J. Anim. Sci., 2019, 64(6):248-254 | DOI: 10.17221/240/2018-CJAS
Abstract: This study provides the first characterization of maternal ancestry and mitochondrial DNA (mtDNA) diversity in the Croatian Spotted goat (CSG), the most important autochthonous goat breed in Croatia. CSG (n = 25) were randomly sampled from seven herds and a 660-bp fragment from the mtDNA D-loop region was sequenced. Those sequences were compared with 122 corresponding GenBank sequences from goat populations in Albania, Austria, Egypt, Greece, Italy, Romania and Switzerland. CSG showed a great polymorphism (only three out of 17 haplotypes were shared) with high a haplotype (Hd = 0.967 ± 0.019) and nucleotide...
Association of polymorphisms in the GH and GHR genes with growth and carcass traits in rabbits (Oryctolagus cuniculus)Original Paper
Łukasz Migdał, Sylwia Pałka, Michał Kmiecik, Olga Derewicka
Czech J. Anim. Sci., 2019, 64(6):255-264 | DOI: 10.17221/27/2019-CJAS
In rabbits, growth and carcass traits are important for the breeding programme. An increasing number of annotated polymorphisms demands validation of their influence on those traits before they can be implemented in breeding practice. Therefore, the aim of this study was to investigate GH c.-78C>T, GHR c.106G>C polymorphisms in the population of Belgian Giant Grey, Termond White, and a crossbreed between New Zealand White and Belgian Giant Grey (NZW × BGG) rabbits. In total 379 animals were genotyped and association analyses with growth traits and carcass traits were conducted. Our results demonstrated that GH c.-78C>T...
Antioxidant defence system in protein fractions of common carp (Cyprinus carpio) seminal plasmaOriginal Paper
Anna Shaliutina-Kolešová, Mo Xian, Rui Nian
Czech J. Anim. Sci., 2019, 64(6):265-271 | DOI: 10.17221/187/2018-CJAS
There are growing evidences that the damage which is caused to the spermatozoa by the reactive oxygen species (ROS) plays a key role in male infertility. The seminal plasma is endowed with many enzymatic and non-enzymatic antioxidants which protect the spermatozoa against oxidative stress. The present study was undertaken to compare the level of superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx) in separated protein fractions of whole common carp Cyprinus carpio seminal plasma. Seminal plasma from six male common carp was separated into four fractions. The fractions differed from each other in their total...
Effect of supplemental lactic acid bacteria on growth performance, glutathione turnover and aflatoxin B1 removal in lambsOriginal Paper
Jianping Wang, Lin Lin, Qingrong Jiang, Wanli Huang, Ning Liu
Czech J. Anim. Sci., 2019, 64(6):272-278 | DOI: 10.17221/5/2019-CJAS
This study investigated the effect of lactic acid bacteria (LAB) on growth performance, glutathione turnover, aflatoxin B1 (AFB1) residue and AFB1-DNA adduct in growing lambs. Diets were a 2 × 2 factorial design, including AFB1 at 0 or 100 µg/kg and LAB at 0 or 3 × 109 cfu/kg. Results showed that AFB1 decreased (P < 0.05) feed intake (FI), body weight gain (BWG), and activities of glutathione (GSH), glutathione s-transferases (GSTs) and glutathione reductase (GR) in liver and duodenal mucosa, whereas these parameters were increased (P < 0.05) by supplemental LAB. AFB1...