Czech J. Anim. Sci., 2019, 64(6):239-247 | DOI: 10.17221/40/2018-CJAS

Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigsOriginal Paper

Wen-Kui Sun1,2, Chi Cheng3, Rui Liu4, Yi-Hui Chen1, Kai Zeng4, Xiao-Hui Chen4, Yi-Ren Gu4, Jiang-Ling Li4, Xue-Bin Lv4, Rong Gao*,1
1 Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China
2 Chengdu Medical College, School of Laboratory Medicine, Chengdu, P.R. China
3 College of Chemistry and Life Science, Chengdu Normal University, Chengdu, P.R. China
4 Sichuan Academy of Animal Science, Chengdu, P.R. China

The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin receptor (GHSR), LEP, leptin receptor (LEPR), SST and CCK) by reverse transcription-qPCR (RT-qPCR). TP generally had higher mRNA expressions of GHSR, LEP, LEPR, SST and CCK genes compared to YP, and expressed lower levels of the GHRL gene in most tissues of the digestive tract. In both breeds, plasma levels of the expressed proteins were more closely correlated with the feed intake and growth than with mRNA levels of the target genes. Our data indicate that TP possess special gene expression patterns in the gastrointestinal tract compared to YP, which is consistent with its unique feed intake and adaptation to harsh environment.

Keywords: gene transcript; hormone level; growth performance; digestive traits

Published: June 30, 2019  Show citation

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Sun W, Cheng C, Liu R, Chen Y, Zeng K, Chen X, et al.. Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs. Czech J. Anim. Sci. 2019;64(6):239-247. doi: 10.17221/40/2018-CJAS.
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