Czech Journal of Animal Science, 2019 (vol. 64), issue 10
Impact of reference population and marker density on accuracy of population imputationOriginal Paper
Anita Kranjčevičová, Eva Kašná, Michaela Brzáková, Josef Přibyl, Luboš Vostrý
Czech J. Anim. Sci., 2019, 64(10):405-410 | DOI: 10.17221/148/2019-CJAS 
The effect of the reference population size and the number of missing single nucleotide polymorphisms (SNPs) on imputation accuracy was determined. The population imputation method using the FImpute software was applied. The dataset used for the purpose of this study was taken from the database of the Holstein Cattle Breeders Association of the Czech Republic. It contains 1000 animals genotyped with the Illumina BovineSNP50 v.2 BeadChip. Two datasets were created, the first containing the original genotypes, including the missing SNPs, the second containing the same genotypes modified to avoid missing data. In these datasets, animals were randomly...
Genetic diversity and population structure of four cattle breeds raised in Turkey using microsatellite markersOriginal Paper
Eymen Demir, Murat Soner Balcioglu
Czech J. Anim. Sci., 2019, 64(10):411-419 | DOI: 10.17221/62/2019-CJAS
In the present study, genetic diversity and population structure of Holstein Friesian and three native cattle breeds of Turkey including Turkish Grey Steppe, Eastern Anatolian Red and Anatolian Black were assessed. Totally 120 individuals of 4 breeds were genotyped using 20 microsatellite markers and 204 different alleles, of which 31 were private alleles, were detected. The average observed and expected heterozygosity values were 0.63 and 0.74, respectively. Observed heterozygosity at the marker level ranged from 0.30 (DRBP1) to 0.88 (ILSTS011), while expected heterozygosity ranged from 0.51 (INRABERN172) to 0.88 (SPS113)....
Comparison of the pituitary gland transcriptome in pregnant and non-pregnant goats (Capra hircus)Original Paper
Qing Quan, Lu Zhu, Qi Zheng, Hao Wu, Jing Jing, Qing Chen, Ya Liu, Fugui Fang, Yunsheng Li, Yunhai Zhang, Yinghui Ling
Czech J. Anim. Sci., 2019, 64(10):420-430 | DOI: 10.17221/141/2019-CJAS
Pregnancy is strictly regulated by neuronal and hormonal factors with an essential role being played by the pituitary gland. We screened for differentially expressed genes (DEGs) in the pituitary that function in goat gestational development. Pregnant (AWGp) and non-pregnant Anhui white goats (AWGn) were analysed by deep-sequencing technology. A total of 12 774 092 and 13 872 327 clear reads were obtained in the AWGp and AWGn libraries, respectively. A total of 2593 genes were labelled as significantly differentially expressed in AWGp compared to AWGn, including 2158 upregulated genes and 435 downregulated genes. These genes included follicle stimulating...
Differences between Jersey and Holstein cows in milking-induced teat prolongation during lactationOriginal Paper
Matúš Gašparík, Luděk Stádník, Jaromír Ducháček, Vladimír Tančin
Czech J. Anim. Sci., 2019, 64(10):431-438 | DOI: 10.17221/145/2019-CJAS
Factors consequential to milking-induced teat prolongation (MITP) were identified. Effects of breed, teat position, lactation number, lactation stage and their interactions were evaluated. The length of each teat before and after milking was measured seven times during lactation in 59 Holstein cows and 45 Jersey cows. Rear teats seemed to prolong more with the exception of rear left teats of Holstein cows. MITP of Holsteins was more balanced among quarters compared to Jerseys, where we observed significantly higher MITP of their rear teats. The pairs mostly had similar reactions even for different teat lengths, therefore for future studies evaluating...