Czech Journal of Animal Science, 2014 (vol. 59), issue 9
Effect of dietary eicosapentaenoic and docosahexaenoic acid on expression of rat liver genes controlling cholesterol homeostasis and on plasma cholesterol levelOriginal Paper
T. Komprda, G. Zorníková, A. Knoll, Z. Vykoukalová, V. Rozíková, O. Škultéty, R. Krobot
Czech J. Anim. Sci., 2014, 59(9):391-398 | DOI: 10.17221/7650-CJAS 
A hypothesis that eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA) lower plasma cholesterol via increased expression of the Insig-1 gene with ensuing decrease of expression of genes coding for 3-hydroxy-3-methyl-glutaryl-CoA reductase (Hmgcr) and low density lipoprotein receptor (Ldlr) was tested in rats fed a diet with 3% of fish oil (FO). Expression of the Insig-1 gene in the liver of the FO-fed rats was 730% (P < 0.05) of the control. However, contrary to the hypothesis, expression of the Hmgcr gene and Ldlr gene was 165% and 210% of the control (P > 0.05). Nevertheless,...
Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickensOriginal Paper
S.K. Bhanja, M. Sudhagar, A. Goel, N. Pandey, M. Mehra, S.K. Agarwal, A. Mandal
Czech J. Anim. Sci., 2014, 59(9):399-408 | DOI: 10.17221/7651-CJAS
The present study was aimed at investigating the role of in ovo administered amino acids: lysine, arginine, threonine or methionine plus cysteine (Met+Cys) in 14-day embryos on expression profile of growth (chicken growth hormone (cGH), insulin like growth factors (IGF) I and II, and mucin) and immunity related genes (IL-2, IL-4, IL-6, IL-12, TNF-α, and IFN-γ). On incubation day (ID) 18, higher (P < 0.01) cGH and mucin gene expression was observed in lysine, threonine, arginine or Met+Cys injected embryos, while IGF-II expression was higher...
Domestic and Interbull information in the single step genomic evaluation of Holstein milk productionOriginal Paper
J. Přibyl, J. Bauer, P. Pešek, J. Přibylová, L. Vostrý, L. Zavadilová
Czech J. Anim. Sci., 2014, 59(9):409-415 | DOI: 10.17221/7652-CJAS
Estimated breeding values and genomic enhanced breeding values for milk production of young genotyped Holstein bulls were predicted using a conventional animal model, ridge regression genomic prediction procedure, genomic best linear unbiased prediction, single-step genomic best linear unbiased prediction, and one-step blending procedures. For prediction, the nation-wide database of domestic Czech production records was combined with deregressed proofs from Interbull files through 2008, which had been transformed by multiple across country evaluation to reflect domestic production conditions. 1259 genotyped bulls had already been proven in 2008. Analyses...
Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of ratsOriginal Paper
V. Vlčková, M. Malinová, B. Koubková, J. Száková, V. Zídek, A. Fučíková, J. Zídková, D. Kolihová, P. Tlustoš
Czech J. Anim. Sci., 2014, 59(9):416-427 | DOI: 10.17221/7653-CJAS
The long-term accumulation of risk elements (As, Cd, Pb) originated from differently contaminated soils in rat organism was investigated during a model two-generation experiment. The effect of soil contamination level, gender, and length of exposure as well as the interactions between risk elements and selected essential macro- and microelements were studied. Rat diet contained 10% of individual soils (based on dry weight): (i) Fluvisol heavily polluted by As, Cd, Zn, and Pb, (ii) Luvisol contaminated by As, Cd, and Zn, and (iii) uncontaminated Chernozem. Male and female Wistar rats used for the experiment were housed in...
Goat mastitis detection using daily records of milk conductivity: comparative results of different algorithmsOriginal Paper
G. Romero, D. Reinemann, M. Alejandro, J.R. Díaz
Czech J. Anim. Sci., 2014, 59(9):428-434 | DOI: 10.17221/7654-CJAS
Milk electrical conductivity is employed for mastitis detection in cows due to its automation, low cost, and infection detectability at early stage. Nevertheless, the number of publications about its use in dairy goats is scarce. The aim of this study was to check and compare the detectability of goat mastitis (sensitivity and specificity) using different algorithms, constructed with individual daily conductivity data from glands, in order to improve the know how about the potential of this variable for goat mastitis detection. A total of 18 goats (8 primiparous and 10 multiparous) free of mastitis were used, and gland milk conductivity was daily monitored....
Effect of hen age, environmental temperature, and oviposition time on egg shell quality and egg shell and serum mineral contents in laying and broiler breeder hensOriginal Paper
E. Tůmová, R.M. Gous, N. Tyler
Czech J. Anim. Sci., 2014, 59(9):435-443 | DOI: 10.17221/7655-CJAS
The aim of the study was to evaluate egg shell quality characteristics, mineral content in the egg shell, and serum mineral concentration during the egg formation process as influenced by oviposition time and ambient temperature at the beginning and end of the laying cycle in laying hens and broiler breeders. Egg shell quality deteriorated significantly at the higher temperature and was lower in broiler breeders than in laying hens. The Ca (P = 0.047) and P (P = 0.018) contents of the egg shell were significantly higher at the higher temperature. The highest (P = 0.028) shell Ca content (352 g/kg) was in eggs laid in the...