Czech J. Anim. Sci., 2014, 59(9):391-398 | DOI: 10.17221/7650-CJAS

Effect of dietary eicosapentaenoic and docosahexaenoic acid on expression of rat liver genes controlling cholesterol homeostasis and on plasma cholesterol levelOriginal Paper

T. Komprda1, G. Zorníková1, A. Knoll2,3, Z. Vykoukalová2, V. Rozíková1, O. Škultéty2, R. Krobot4
1 Department of Food Technology, Mendel University in Brno, Brno, Czech Republic
2 Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Brno, Czech Republic
3 CEITEC MENDELU, Mendel University in Brno, Brno, Czech Republic
4 Department of Animal Nutrition and Forage Production, Mendel University in Brno, Brno, Czech Republic

A hypothesis that eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA) lower plasma cholesterol via increased expression of the Insig-1 gene with ensuing decrease of expression of genes coding for 3-hydroxy-3-methyl-glutaryl-CoA reductase (Hmgcr) and low density lipoprotein receptor (Ldlr) was tested in rats fed a diet with 3% of fish oil (FO). Expression of the Insig-1 gene in the liver of the FO-fed rats was 730% (P < 0.05) of the control. However, contrary to the hypothesis, expression of the Hmgcr gene and Ldlr gene was 165% and 210% of the control (P > 0.05). Nevertheless, FO in the diet decreased (P < 0.05) plasma cholesterol of rats by 10% (from 1.19 to 1.07 mmol/l); it was therefore concluded that the cholesterol-lowering effect of EPA+DHA is at least partly based on mechanisms other than tested in the present experiment.

Keywords: PPARα; SREBP-2; Insig-1; cholesterol; PUFAn-3; rats

Published: September 30, 2014  Show citation

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Komprda T, Zorníková G, Knoll A, Vykoukalová Z, Rozíková V, Škultéty O, Krobot R. Effect of dietary eicosapentaenoic and docosahexaenoic acid on expression of rat liver genes controlling cholesterol homeostasis and on plasma cholesterol level. Czech J. Anim. Sci. 2014;59(9):391-398. doi: 10.17221/7650-CJAS.
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