Czech Journal of Animal Science, 2013 (vol. 58), issue 7

Analysis of longevity in the Slovak Pinzgau population - extension to the animal modelOriginal Paper

G. Mészáros, O. Kadlečík, R. Kasarda, J. Sölkner

Czech J. Anim. Sci., 2013, 58(7):289-295 | DOI: 10.17221/6858-CJAS  

Breeding values for length of productive life in Slovak Pinzgau cattle were estimated using survival analysis. As the results were corrected for milk production, the final breeding values represented the ability of cows to avoid culling from reasons other than milk production. In addition to the relative milk yield, the risk of culling was also studied in connection with the herd and year of calving, parity and stage of lactation, herd size change and age at the first calving. Among the fixed effects, the low milk production, high age at the first calving, and decreasing herd size were associated with increased risk of culling. The risk was non-linear...

Fresh and ensiled forages as a source of estrogenic equol in bovine milk: a reviewOriginal Paper

P. Kalač

Czech J. Anim. Sci., 2013, 58(7):296-303 | DOI: 10.17221/6859-CJAS  

Isoflavan equol has recently been assessed in human nutrition as a potent estrogen with various health benefits. It is produced by intestinal microflora from isoflavones (IF) formononetin and daidzein. These dietary precursors are present particularly in soy products. However, only 20-35% of the Western adult population is capable of producing equol from the ingested IF. Cow's milk was proved to be an important source of equol produced from the IF in the rumen and intestines. Among forages, red clover (Trifolium pratense) is by far the most important source of both the IF. Their contents in fresh red clover forage are affected by variety and...

Impact of crude protein content in silage and concentrate on protein and fatty acid profiles in bovine milkOriginal Paper

M. Johansson, O. Placha, J. Pickova, A. Andrén, G. Zamaratskaia, E. Spörndly, M. Åkerstedt

Czech J. Anim. Sci., 2013, 58(7):304-312 | DOI: 10.17221/6860-CJAS  

Two concentrates, one protein-rich and one based on cereals, were combined with two silages with a crude protein content of 17 and 13% of dry matter (DM), respectively to give four different diets for dairy cows. Milk content of caseins (αS1-, αS2-, β-, and κ-casein) and whey proteins (α-lactalbumin (α-LA) and β-lactoglobulin (β-LG)) and the fatty acid profile of milk were analyzed before the start and on four occasions during the experiment. Milk analyses showed that diet had no influence on the protein profile of the milk. However, a significant increase of α-linolenic acid,...

Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish speciesOriginal Paper

A. Shaliutina-Kolešová, I. Gazo, J. Cosson, O. Linhart

Czech J. Anim. Sci., 2013, 58(7):313-320 | DOI: 10.17221/6861-CJAS  

Oxidant and antioxidant activity in seminal plasma was compared with that in spermatozoa of teleost (common carp Cyprinus carpio and brook trout Salvelinus fontinalis) and chondrostean (Russian sturgeon Acipenser gueldenstaedtii, Siberian sturgeon Acipenser baerii, and sterlet Acipenser ruthenus) fishes. No differences were found between seminal plasma and spermatozoa in the level of thiobarbituric-acid-reactive substance (0.24 ± 0.08 to 0.33 ± 0.04 nmol/mg proteins) in Russian sturgeon, Siberian sturgeon, and sterlet. Carbonyl protein concentration was significantly higher in spermatozoa...

Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traitsOriginal Paper

X.P. An, J.X. Hou, H.B. Zhao, L. Bai, J.Y. Peng, C.M. Zhu, Q.M. Yan, Y.X. Song, J.G. Wang, B.Y. Cao

Czech J. Anim. Sci., 2013, 58(7):321-327 | DOI: 10.17221/6862-CJAS  

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C- allele were 0.79-0.85, and frequencies of C+ allele were 0.21-0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70-0.72, and frequencies of T allele were 0.30-0.28. Compared with...

Selenite and selenate affect the fatty acid profile in in vitro incubated ovine ruminal fluid containing linseed oilOriginal Paper

M. Czauderna, J. Kowalczyk, M. Marounek

Czech J. Anim. Sci., 2013, 58(7):328-341 | DOI: 10.17221/6863-CJAS  

The influence of selenite (SeIV) or selenate (SeVI) added to ovine ruminal fluid containing linseed oil (LO) on the profile of fatty acids (FA), particularly conjugated linoleic acid (CLA) isomers, was investigated. The ruminal fluid was incubated in vitro at 39°C under CO2 either alone (the control fluid) or with LO (3.3 mg/ml) or with a combination of LO with either a low (0.167 μg/ml) or high (1.67 μg/ml) level of Se as SeIV or SeVI. LO added to ruminal fluids also provides an extra source of energy. The tubes with the examined fluids were removed after 0, 6, 12, 18, or 24 h of...