Czech J. Anim. Sci., 2013, 58(7):321-327 | DOI: 10.17221/6862-CJAS

Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traitsOriginal Paper

X.P. An, J.X. Hou, H.B. Zhao, L. Bai, J.Y. Peng, C.M. Zhu, Q.M. Yan, Y.X. Song, J.G. Wang, B.Y. Cao
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C- allele were 0.79-0.85, and frequencies of C+ allele were 0.21-0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70-0.72, and frequencies of T allele were 0.30-0.28. Compared with goats with DGAT1 C-C-, those with C-C+ genotype had greater milk fat (P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P < 0.05). The results showed that does with C-C-CT and C-C+CT yielded more milk than those with C-C-CC(P < 0.05). In addition, does with C-C+CT had the highest milk fat in comparison with other combination genotypes (P < 0.05).

Keywords: milk yield; fat percentage; dairy goat; SNP; PCR-RFLP; SSCP; additive effect

Published: July 31, 2013  Show citation

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An XP, Hou JX, Zhao HB, Bai L, Peng JY, Zhu CM, et al.. Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits. Czech J. Anim. Sci. 2013;58(7):321-327. doi: 10.17221/6862-CJAS.
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    References

    1. Amills M., Angiolillo A., Urrutia B., Domenech A., Sastre Y., Badaoui B., Jordana J. (2007): Identification of a single nucleotide polymorphism at intron 16 of the caprine acylcoenzyme A: Diacylglycerol acyltransferase 1 (DGAT1) gene. Journal of Dairy Research, 74, 47-51. Go to original source... Go to PubMed...
    2. An X.P., Song S.G., Hou J.X., Zhu C.M., Peng J.X., Liu X.Q., Liu H.Y., Xiao W.P., Zhao H.P., Bai L., Wang J.G., Song Y.X., Cao B.Y. (2011): Polymorphism identification in goat DGAT2 gene and association analysis with milk yield and fat percentage. Small Ruminant Research, 100, 107-112. Go to original source...
    3. Antoniou E., Hirts B.J., Grosz M., Skidmorec J. (1999): A single strand conformation polymorphism in the bovine gene STAT5A. Animal Genetics, 30, 225-244. Go to original source... Go to PubMed...
    4. Brym P., Kamiński S., Ruść A. (2004): New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. Journal of Applied Genetics, 45, 445-452. Go to PubMed...
    5. Cases S., Stone S.J., Zhou P., Yen E., Tow B., Lardizabal K.D., Voelker T., Farese R.V. (2001): Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase and related family members. Journal of Biological Chemistry, 276, 38870-38876. Go to original source... Go to PubMed...
    6. Darnell J.E. (1997): STATs and gene regulation. Science, 277, 1630-1635. Go to original source... Go to PubMed...
    7. Darnell J.E., Kerr I.M., Stark G.R. (1994): Jak-Stat pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science, 264, 1415-1421. Go to original source... Go to PubMed...
    8. Dekkers J.C. (2004): Commercial application of marker- and gene-assisted selection in livestock: strategies and lessons. Journal of Animal Science, 82, E313-E328. Go to PubMed...
    9. Erhardt G., Caroli A., Rizzi R., Luhken G. (2010): Milk protein genetic variation and casein haplotype structure in the Original Pinzgauer cattle. Journal of Dairy Science, 93, 1260-1265. Go to original source... Go to PubMed...
    10. Flisikowski K., Strzałkowska N., Słoniewski K., Krzyżewki J., Zwierzchowski L. (2004): Association of a sequence nucleotide polymorphism in exon 16 of the STAT5A gene with milk production traits in Polish Black-and-White (Polish Friesian) cows. Animal Science Papers and Reports, 22, 515-522.
    11. Giannoulia K., Haralampidis K., Poghosyan Z., Murphy D.J., Hatzopoulos P. (2000): Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues. Biochemical Society Transactions, 28, 695-697. Go to original source... Go to PubMed...
    12. Goldammer T., Meyer L., Seyfert H.M., Brunner R.M., Schwerin M. (1997): STAT5A encoding gene maps to chromosome 19 in cattle and goat and to chromosome 11 in sheep. Mammalian Genome, 8, 705-706. Go to original source... Go to PubMed...
    13. Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni M., Reid S., Simon P., Spelman R., Georges M., Snell R. (2002): Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research, 12, 222-231. Go to original source... Go to PubMed...
    14. Hatzopoulos P., Banilas G., Karampelias M., Makariti I., Kourti A. (2011): The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1. Journal of Experimental Botany, 62, 521-532. Go to original source... Go to PubMed...
    15. He X., Chu M.X., Qiao L., He J.N., Wang P.Q., Feng T., Di R., Cao G.L., Fang L., An Y.F. (2012): Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows. Molecular Biology Reports, 39, 2901-2907. Go to original source... Go to PubMed...
    16. Ji Y.T., Qu C.Q., Cao B.Y. (2007): An optimal method of DNA silver staining in polyacrylamide gels. Electrophoresis, 28, 1173-1175. Go to original source... Go to PubMed...
    17. Mullenbach R., Lagoda P.J., Welter C. (1989): An efficient salt chloroform extraction of DNA from blood and tissue. Trends in Genetics, 5, 391. Go to PubMed...
    18. Parmentier I., Portetellea D., Gengler N., Prandic A., Bertozzia C., Vleuricka L., Gilsond R., Renaville R. (1999): Candidate gene markers associated with somatotropic axis and milk selection. Domestic Animal Endocrinology, 17, 139-148. Go to original source... Go to PubMed...
    19. Sadeghi M., Shahrbabak M.M., Mianj G.R., Javaremi A.N. (2009): Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls. Livestock Science, 123, 97-100. Go to original source...
    20. Schennink A., Bovenhuis H., Leon-Kloosterziel K.M., van Arendonk J.A.M., Visker M. (2009): Effect of polymorphisms in the FASN, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition. Animal Genetics, 40, 909-916. Go to original source... Go to PubMed...
    21. Selvaggi M., Dario C., Normanno G., Celano G.V., Dario M. (2009): Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. Journal of Dairy Research, 76, 441-445. Go to original source... Go to PubMed...
    22. Seyfert H.M., Pitra C., Meyer L., Brunner R.M., Wheeler T.T., Molenaar A., McCracken J.Y., Herrmann J., Thiesen H.J., Schwerin M. (2000): Molecular characterization of STAT5A- and STAT5B-encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains. Journal of Molecular Evolution, 50, 550-561. Go to original source... Go to PubMed...
    23. Spelman R.J., Ford C.A., McElhinney P., Gregory G.C., Snell R.G. (2002): Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science, 85, 3514-3517. Go to original source... Go to PubMed...
    24. Van der Werf J.H.J., Marshall K., Lee S. (2007): Methods and experimental designs for detection of QTL in sheep and goats. Small Ruminant Research, 70, 21-31. Go to original source...
    25. Wakao H., Gouilleux F., Groner B. (1994): Mammary-gland factor (Mgf) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO Journal, 13, 2182-2191. Go to original source... Go to PubMed...
    26. Winter A., Kramer W., Werner F.A.O., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G., Fries R. (2002): Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA : diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences, 99, 9300-9305. Go to original source... Go to PubMed...

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