Czech J. Anim. Sci., 2013, 58(7):304-312 | DOI: 10.17221/6860-CJAS

Impact of crude protein content in silage and concentrate on protein and fatty acid profiles in bovine milkOriginal Paper

M. Johansson1, O. Placha1, J. Pickova1, A. Andrén1, G. Zamaratskaia1, E. Spörndly2, M. Åkerstedt1
1 Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden
2 Department of Animal Nutrition and Management, Kungsängen Research Centre, Uppsala, Sweden

Two concentrates, one protein-rich and one based on cereals, were combined with two silages with a crude protein content of 17 and 13% of dry matter (DM), respectively to give four different diets for dairy cows. Milk content of caseins (αS1-, αS2-, β-, and κ-casein) and whey proteins (α-lactalbumin (α-LA) and β-lactoglobulin (β-LG)) and the fatty acid profile of milk were analyzed before the start and on four occasions during the experiment. Milk analyses showed that diet had no influence on the protein profile of the milk. However, a significant increase of α-linolenic acid, 13 and 39%, was obtained on the high protein concentrate feed and on the silage higher in crude protein, respectively. Cows on the protein-rich concentrate diet increased the proportion of conjugated linoleic acid by 53%. Linoleic acid was not affected by the diet.

Keywords: milk proteins; feed protein; clover; casein; CLA; α-linolenic acid; capillary electrophoresis

Published: July 31, 2013  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Johansson M, Placha O, Pickova J, Andrén A, Zamaratskaia G, Spörndly E, Åkerstedt M. Impact of crude protein content in silage and concentrate on protein and fatty acid profiles in bovine milk. Czech J. Anim. Sci. 2013;58(7):304-312. doi: 10.17221/6860-CJAS.
Download citation

References

  1. Åkerstedt M., Wredle E., Lam V., Johansson M. (2012): Protein degradation in bovine milk caused by Streptococcus agalactiae. Journal of Dairy Research, 79, 297-303. Go to original source... Go to PubMed...
  2. Al-Mabruk R.M., Beck N.F.G., Dewhurst R.J. (2004): Effects of silage species and supplemental vitamin E on the oxidative stability of milk. Journal of Dairy Science, 87, 406-412. Go to original source... Go to PubMed...
  3. Bauman D.E., Griinari J.M. (1999): Biosynthesis of CLA and its incorporation into meat and milk of ruminants. Journal of Animal Science, 77, 117. Go to original source...
  4. Bertilsson J., Murphy M. (2003): Effects of feeding clover silages on feed intake, milk production and digestion in dairy cows. Grass and Forage Science, 58, 309-322. Go to original source...
  5. Bertilsson J., Dewhurst R.J.Tuori M. (2002): Effects of legume silages on feed intake, milk production and nitrogen efficiency. Sonderheft 234, Special issue, Landbauforschung Völkenrode, FAL Agricultural Research, 39-45.
  6. Chilliard Y., Ferlay A. (2004): Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reproduction Nutrition Development, 44, 467-492. Go to original source... Go to PubMed...
  7. Chilliard Y., Ferlay A., Mansbridge R.M., Doreau M. (2000): Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. Annales de Zootechnie, 49, 181-205. Go to original source...
  8. Dewhurst R.J., Evans R.T., Scollan N.D., Moorby J.M., Merry R.J., Wilkins R.J. (2003): Comparison of grass and legume silages for milk production. 2. In vivo and in sacco evaluations of rumen function. Journal of Dairy Science, 86, 2612-2621. Go to original source... Go to PubMed...
  9. European Commission (2008): Commission Regulation (EC) No 889/2008 of 5 September 2008. Official Journal of the European Union, L250, 1-84.
  10. Hallén E., Allmere T., Lundén A., Andrén A. (2009): Effect of genetic polymorphism of milk proteins on rheology of acid-induced milk gels. International Dairy Journal, 19, 399-404. Go to original source...
  11. Hallén E., Lundén A., Allmere T., Andrén A. (2010): Casein retention in curd and loss of casein into whey at chymosin-induced coagulation of milk. Journal of Dairy Research, 77, 71-76. Go to original source... Go to PubMed...
  12. Hara A., Radin N.S. (1978): Lipid extraction of tissues with a low toxicity solvent. Analytical Biochemistry, 90, 420-426. Go to original source... Go to PubMed...
  13. Heck J.M.L., Olieman C., Schennink A., van Valenberg H.J.F., Visker M.H.P.W., Meuldijk R.C.R., van Hooijdonk A.C.M. (2008): Estimation of variation in concentration, phosphorylation and genetic polymorphism of milk proteins using capillary zone electrophoresis. International Dairy Journal, 18, 548-555. Go to original source...
  14. Johansson B., Holtenius K. (2008): Organic milk production based entirely on grassland feeds. In: Hopkins A., Gustafsson T., Bertilsson J., Dalin G., Nilsdotter-Linde N., Spörndly E. (eds): Biodiversity and Animal Feed - Future Challenges for Grassland Production. Proc. 22nd General Meeting of the European Grassland Federation, Uppsala, Sweeden, 825-827.
  15. Kalač P., Samková E. (2010): The effects of feeding various forages on fatty acid composition of bovine milk fat: A review. Czech Journal of Animal Science, 55, 521-537. Go to original source...
  16. Kelly A.L., O'Flaherty F., Fox P.F. (2006): Indigenous proteolytic enzymes in milk: a brief overview of the present state of knowledge. International Dairy Journal, 16, 563-572. Go to original source...
  17. Lee M.R.F., Connelly P.L., Tweed J.K.S., Dewhurst R.J., Merry R.J., Scollan N.D. (2006): Effects of high-sugar ryegrass silage and mixtures with red clover silage on ruminant digestion. 2. Lipids. Journal of Animal Science, 84, 3061-3070. Go to original source... Go to PubMed...
  18. Leiber F., Kreuzer M., Nigg D., Wettstein H.R., Scheeder M.R.L. (2005): A study on the causes for the elevated n-3 fatty acids in cow's milk of alpine origin. Lipids, 40, 191-202. Go to original source... Go to PubMed...
  19. MacGibbon A.K.H., Taylor M.W. (2006): Composition and structure of bovine milk lipids. In: Fox P.F., McSweeney P.L.H. (eds): Advanced Dairy Chemistry. Volume 2: Lipids. 3rd Ed. Springer, New York, 1-41. Go to original source...
  20. Mattila-Sandholm T., Saarela M. (2003): Functional Dairy Products. 1st Ed. Woodhead Publishing Ltd., Cambridge, UK. Go to original source...
  21. Miralles B., Ramos M., Amigo L. (2003): Influence of proteolysis of milk on the whey protein to total protein ratio as determined by capillary electrophoresis. Journal of Dairy Science, 86, 2813-2817. Go to original source... Go to PubMed...
  22. Olmes Colmenero J.J., Broderick G.A. (2006): Effect of dietary crude protein concentration on ruminal nitrogen metabolism in lactating dairy cows. Journal of Dairy Science, 89, 1694-1703. Go to original source... Go to PubMed...
  23. Palmquist D.L., Beaulieu A.D., Barbano D.M. (1993): Feed and animal factors influencing milk fat composition. Journal of Dairy Science, 76, 1753-1771. Go to original source... Go to PubMed...
  24. Rosati A., Aumaitre A. (2004): Organic dairy farming in Europe. Livestock Production Science, 90, 41-51. Go to original source...
  25. Sjaunja L.O., Baevre L., Junkkarinen L., Pedersen J., Setälä J. (1990): A Nordic proposal for an energy corrected milk (ECM) formula. In: Proc. 27 th Biennial Session of the International Committee for Animal Recording (ICAR), Paris, France, 156-192.
  26. Spörndly R. (ed.) (2003): Feed tables for ruminants 2003. Report No. 257, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, Sweden, 1-96. (in Swedish)
  27. Theurer C.B., Huber J.T., Santos F.A.P. (1995): Feeding and managing for maximal milk protein. In: Proc. Southwest Nutrition and Management Conference, University of Arizona, Tucson, USA, 59-67.
  28. Wedholm A., Larsen L.B., Lindmark-Månsson H., Karlsson A.H., Andrén A. (2006): Effect of protein composition on the cheese-making properties of milk from individual dairy cows. Journal of Dairy Science, 89, 3296-3305. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.