Czech Journal of Animal Science, 2011 (vol. 56), issue 1
The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds
Q.L. Xu, G.W. Tang, Q.L. Zhang, Y.K. Huang, Y.X. Liu, K. Quan, K.Y. Zhu, C.X. Zhang
Czech J. Anim. Sci., 2011, 56(1):1-6 | DOI: 10.17221/231/2009-CJAS 
The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P...
Superovulation following follicular synchronization with GnRH at random stages of the oestrous cycle in heifers
H. Kohram, V. Vahedi, S. Nasrollahi, A. Farahavar
Czech J. Anim. Sci., 2011, 56(1):7-14 | DOI: 10.17221/325/2009-CJAS
The objective of this study was to develop a superovulatory program based on synchronization of follicular waves with GnRH which could be applied regardless of the stage of the oestrous cycle. 36 heifers were subjected to this experiment and GnRH (Cystorelin, 200 µg) was applied between Days 0 and 7 (n = 15), 8 and 12 (n = 8) or 13 and 20 (n = 13) of the oestrous cycle. Four days after GnRH treatment, all follicles ≥ 6 mm of heifers (n) were either punctured (n = 21) or left intact (n = 15). All heifers were superstimulated from Day 6 to Day 10 after GnRH treatment with 320 mg Folltropin-V....
Profile of the body surface proteolytic systém in Apis mellifera quee
A.J. Strachecka, M.M. Gryzińska, M. Krauze, K. Grzywnowicz
Czech J. Anim. Sci., 2011, 56(1):15-22 | DOI: 10.17221/150/2009-CJAS
The proteolytic system on the body surface of the honey bee has been insufficiently researched. In this study the body surface proteolytic activity was examined in queens at various developmental stages (eggs, larvae, pupae and imagines) in different seasons (spring, summer, autumn, winter). Extracts of the body surface material with water and detergent were used for an in vitro analysis of the proteolytic activity and protease inhibitor level assaying, as well as for an electrophoretic separation of the extracts in polyacrylamide gels. The following methods were used: protein content testing by the Lowry method (modified by Schacterle-Pollack),...
A new internal standard for HPLC assay of conjugated linoleic acid in animal tissues and milk
M. Czauderna, J. Kowalczyk, M. Marounek, J.P. Michalski, A.J. Rozbicka-Wieczorek, K.A. Krajewska
Czech J. Anim. Sci., 2011, 56(1):23-29 | DOI: 10.17221/336/2009-CJAS
A new method for the quantification of underivatized conjugated linoleic acid (CLA) isomers and CLA-metabolites by silver ion liquid chromatography (Ag+-HPLC) with photodiode array detection (DAD) is described. Conjugated fatty acids (CFA) and sorbic acid as the internal standard (IS) were separated on two 5 μm Chrompac ChromSpher Lipids columns (250 × 4.6 mm). Biological samples were hydrolyzed with 1M KOH in methanol and 2M KOH in water at room temperature for 12 h. Hydrolyzates were acidified and the free fatty acids were extracted with dichloromethane. The organic solvent was removed and then the residue was re-dissolved in hexane...
Effect of dietary antibacterial peptide and zinc-methionine on performance and serum biochemical parameters in piglets
J.H. Wang, C.C. Wu, J. Feng
Czech J. Anim. Sci., 2011, 56(1):30-36 | DOI: 10.17221/341/2009-CJAS
The objective of this study was to evaluate the effect of dietary supplementation of antibacterial peptide and zinc methionine on performance and some serum biochemical parameters of weaned piglets. Rongchang male piglets (28 days of age, initial weight 8.4 ± 0.65 kg) were used. All piglets were randomly allotted to four diets including the control group, the antibacterial peptide (AP) group containing 10 mg antibacterial peptide/kg diet, the zinc methionine (Zn-Met) group with 1 200 zinc methionine/kg diet (equal to 200 mg Zn2+/kg diet), the zinc methionine/antibacterial peptide (Zn/AP) group containing 10 mg antibacterial peptide...
The effect of feeding live yeast cultures on ruminal pH and redox potential in dry cows as continuously measured by a new wireless device
L. Křížová, M. Richter, J. Třináctý, J. Říha, D. Kumprechtová
Czech J. Anim. Sci., 2011, 56(1):37-45 | DOI: 10.17221/39/2010-CJAS
An experiment was carried out on four dry Holstein cows fitted with rumen cannulas that were divided into two groups. The crossover design experiment was divided into 4 periods of 3 weeks. Each period consisted of a 17-day preliminary period followed by a 4-day experimental period. Cows were fed twice daily the total mixed ration based on maize silage and concentrate. Control cows (Control) received the basal diets while experimental animals (Yeast) received the basal diet supplemented with 3.0 g of live yeast (BIOSAF Sc 47, Lesaffre, France) at each feeding. During each experimental period ruminal pH and redox potential (Eh) were monitored continuously...