Czech J. Anim. Sci., 2011, 56(1):1-6 | DOI: 10.17221/231/2009-CJAS

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

Q.L. Xu, G.W. Tang, Q.L. Zhang, Y.K. Huang, Y.X. Liu, K. Quan, K.Y. Zhu, C.X. Zhang
Department of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. China

The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P < 0.05), muscle marbling score (P < 0.05) and intramuscular fat content (IMF; P < 0.05). Thus we suggested that the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep.

Keywords: sheep; FABP4 gene; polymorphism; intramuscular fat

Published: January 31, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Xu QL, Tang GW, Zhang QL, Huang YK, Liu YX, Quan K, et al.. The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds. Czech J. Anim. Sci. 2011;56(1):1-6. doi: 10.17221/231/2009-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adida A., Spener F. (2006): Adipocyte-type fatty acidbinding protein as inter-compartmental shuttle for peroxisome proliferators activated receptor γ agonists in cultured cell. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1761, 172-181. Go to original source... Go to PubMed...
    2. Barillet F., Arranz J.J., Carta A. (2005): Mapping quantitative trait loci for milk production and genetic polymorphisms of milk protein in dairy sheep. Genetics, Selection, Evolution, 37, S109-S123. Go to original source... Go to PubMed...
    3. Bassam B.J., Caetano-Anollés G., Gresshoff P.M. (1991): Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83. Go to original source... Go to PubMed...
    4. Bendixen E. (2005): The use of proteomics in meat science. Meat Science, 71, 138-149.  Go to original source... Go to PubMed...
    5. Bligh E.G., Dyer W.J. (1959): A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917. Go to original source... Go to PubMed...
    6. Damcott C.M., Moffett S.P., Feingold E., Barmada M.M., Marshall J.A., Hamman R.F., Ferrell R.E. (2004): Genetic variation in fatty acid-binding protein-4 and peroxisome proliferator-activated receptor gamma interactively influence insulin sensitivity and body composition in males. Metabolism - Clinical and Experimental, 53, 303-309. Go to original source... Go to PubMed...
    7. Damon M., Louveau I., Lefaucheur L., Lebret B., Vincent A., Leroy P., Sanchez M.P., Herpin P., Gondret F. (2006): Number of intramuscular adipocytes and fatty acid binding protein-4 content are significant indicators of intramuscular fat level in crossbred Large White × Duroc pigs. Journal of Animal Science, 84, 1083-1092. Go to original source... Go to PubMed...
    8. Dickerson G.E., Glimp H.A., Tuma H.J., Gregory K.E. (1972): Breeds growth and carcass characteristics of ram lambs of seven genetic resources for efficient meat production in sheep. Journal of Animal Science, 34, 940-951. Go to original source...
    9. Erkens T., Rohrer G.A., Van Zeveren A., Peelman L.J. (2009): SNP detection in the porcine PPARGC1A promoter region and 3'UTR, and an association analysis in a Landrace - Duroc - Yorkshire population. Czech Journal of Animal Science, 54, 408-416. Go to original source...
    10. Falconer D.S., Mackay T.F.C. (1996): Introduction to Quantitative Genetics. 4th ed. Longman Group Essex, Essex, UK, 119.
    11. Fiems L.Q., De Campeneere S., De Smet S., Van de Voorde G., Vanacker G.M., Boucqué C.V. (2000): Relationship between fat depots in carcasses of beef bulls and effect on meat colour and tenderness. Meat Science, 56, 41-47. Go to original source... Go to PubMed...
    12. Gerbens F., Verburg F.J., Van Moerkerk H.T., Engel B., Buist W., Veerkamp J.H., te Pas M.F. (2001): Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs. Journal of Animal Science, 79, 347-354. Go to original source... Go to PubMed...
    13. Honikel K.O. (1998): Reference methods for the assessment of physical characteristics of meat. Meat Science, 49, 457-477. Go to original source... Go to PubMed...
    14. Huff-Lonergan E., Baas T.J., Malek M., Dekkers J.C., Prusa K., Rothschild M.F. (2002): Correlations among selected pork quality traits. Journal of Animal Science, 80, 617-627. Go to original source... Go to PubMed...
    15. Mercadé A., Pérez-Enciso M., Varona L., Alves E., Noguera J.L., Sánchez A., Folch J.M. (2006): Adipocyte fatty-acid binding protein is closely associated to the porcine FAT1 locus on chromosome 4. Journal of Animal Science, 84, 2907-2913. Go to original source... Go to PubMed...
    16. Michal J.J., Zhang Z.W., Gaskins C.T., Jiang Z. (2006): The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu × Limousin F2 crosses. Animal Genetics, 37, 400-402. Go to original source... Go to PubMed...
    17. Nechtelberger D., Pires V., Soolknet J., Brem G., Mueller M., Mueller S. (2001): Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs. Journal of Animal Science, 79, 2798-2804.
    18. Ogino T., Moralejo D.H., Kose H., Yamada T., Matsumoto K. (2003): Serum leptin concentration is linked to chromosomes 2 and 6 in the OLETF rat, an animal model of type 2 diabetes with mild obesity. Mammalian Genome, 14, 239-244. Go to original source... Go to PubMed...
    19. Ojeda A., Rozas J., Folch J.M., Pérez-Enciso M. (2006): Unexpected high polymorphism at the FABP4 gene unveils a complex history for pig populations. Genetics, 174, 2119-2127. Go to original source... Go to PubMed...
    20. Ouali A., Herrera-Mendze C.H., Coulis G., Becila S., Boudjellal A., Aubry L., Sentandreu M.A. (2006): Revisiting the conversion of muscle into meat and the underlying mechanisms. Meat Science, 74, 44-58. Go to original source... Go to PubMed...
    21. Riley R.R., Savell J.W., Murphey C.E., Smith G.C., Stiffler D.M., Cross H.R. (1983): Palatability of beef from steers and young bull carcass as influenced by electrical stimulation, subcutaneous fat thickness and marbling. Journal of Animal Science, 56, 592-597. Go to original source...
    22. Sambrook J., Fritsch E.F., Maniatis T. (1989): Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York, USA.
    23. Savell J.W., Cross H.R. (1988): The role of the fat in the palatability of beef, pork, and lamb. In: Designing Foods. Animal Product Options in the Marketplace. National Academy Press, Washington, USA, 345-355.
    24. Stupka R., Èítek J., ©prysl M., Okrouhlá M., Kure¹ D., Líkaø K. (2008): Effect of weight and sex on intramuscular fat amounts in relation to the formation of selected carcass cuts in pigs. Czech Journal of Animal Science, 53, 506-514. Go to original source...
    25. Wimmers K., Murani E., Schellander K., Ponsuksili S. (2005): Combining QTL and expression-analysis: identification of functional positional candidate genes for meat quality and carcass traits. Archiv Tierzucht, 48, 23-31.
    26. Xing Y.Z., Deng Y.C. (1999): Principle and Method of Swine Testing. Chinese Agriculture Press, Beijing, China.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content