Czech Journal of Animal Science, 2009 (vol. 54), issue 10
Rumen fermentation characteristics in pre-weaning calves receiving yeast culture supplements
B. Hučko, V.A. Bampidis, A. Kodeš, V. Christodoulou, Z. Mudřik, K. Poláková, V. Plachý
Czech J. Anim. Sci., 2009, 54(10):435-442 | DOI: 10.17221/1674-CJAS 
In an experiment with 45 neonatal male Holstein calves, effects of yeast cultures Yea-Sacc® 1026 (SC) containing Saccharomyces cerevisiae (strain 1026) and Vitex (KF) containing Kluyveromyces fragilis (strain Jürgensen) on rumen fermentation characteristics were determined. From day 4 to day 56 of age, the calves were allocated to one of the three dietary treatments (Control, SC, and KF) of 15 animals each, placed in individual pens, and fed 4 l of whole milk twice daily and a basal concentrate mixture ad libitum as calf starter feeds. The control treatment was not supplemented with yeast culture. The yeast culture supplements...
The visfatin (NAMPT; PBEF1) gene polymorphisms and associations with meat performance traits in three pig breeds kept in the Czech Republic
J. Zrůstová, A. Knoll, T. Urban, S. Čepica
Czech J. Anim. Sci., 2009, 54(10):443-447 | DOI: 10.17221/1673-CJAS
Visfatin encoded by the NAMPT gene (nicotinamide phosphoribosyltransferase, formerly also known as PBEF - pre-B cell colony-enhancing factor) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite SCZ004 was detected within intron 9 of the NAMPT gene. In Czech Large White (n = 95) frequencies of alleles 282, 286, 287, 299, and 304 were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele 286 was predominant also in Landrace (n = 11) and Black Pied Prestice (n = 11) breeds. Association analysis was carried out...
Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
Y. Tao, W. Han, M. Zhang, Y. Zhang, J. Fang, J. Liu, R. Zhang, H. Chen, F. Fang, N. Tian, D. Huo, Y. Liu, F. Li, J. Ding, P. Maddox-Hyttel, X. Zhang
Czech J. Anim. Sci., 2009, 54(10):448-460 | DOI: 10.17221/1672-CJAS
The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibroblasts derived from the ear skin of a Boer goat while the recipient ooplasm was in vitro matured oocytes of Huanghuai white goat, an Anhui native goat species. The reconstructed embryos were cultured in vitro and then the morphologically normal embryos were transferred to the surrogates. The reconstructed embryos were surgically transferred into 37 recipient surrogates, Huanghuai white goats with natural oestrus. Five of them were treated...
Analysis of genetic polymorphism in six meat sheep breeds and genetic distances between them
R.J. Chen, Z.P. Yang, D.J. Ji, D.Y. Qu, Y.L. Li, Y.J. Mao, D.L. Huang
Czech J. Anim. Sci., 2009, 54(10):461-467 | DOI: 10.17221/1671-CJAS
The genomes of 6 sheep populations (Dorset, Texel, Black-Suffolk, Tan sheep, Small-tailed Han sheep, Tan and Small-tailed Han hybrid sheep) were screened using 7 microsatellite DNA markers to estimate the genetic diversities and genetic distances among these populations. About 105 alleles were detected at 7 loci in 6 populations. The average observed and expected heterozygosity ranged from 0.2901 to 0.4534 and from 0.8007 to 0.8737 in 6 sheep populations. The expected heterozygosity of each population was much higher than the observed heterozygosity. The mean polymorphism information content (PIC) value of populations ranged from 0.7188 to 0.8546....
Study of genetic differences among Slovak Tsigai populations using microsatellite markers
S. Kusza, E. Gyarmathy, J. Dubravska, I. Nagy, A. Jávor, S. Kukovics
Czech J. Anim. Sci., 2009, 54(10):468-474 | DOI: 10.17221/1670-CJAS
In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of FST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin...