Czech J. Anim. Sci., 2009, 54(10):468-474 | DOI: 10.17221/1670-CJAS

Study of genetic differences among Slovak Tsigai populations using microsatellite markers

S. Kusza1, E. Gyarmathy2, J. Dubravska3, I. Nagy4, A. Jávor1, S. Kukovics5
1 Institute of Animal Science, University of Debrecen, Centre of Agriculture Sciences and Engineering, Debrecen, Hungary
2 Slovak University of Agriculture, Nitra, Slovak Republic
3 Ministry of Agriculture of the Slovak Republic, Bratislava, Slovak Republic
4 Agricultural Biotechnology Centre, Gödöllö, Hungary
5 Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of FST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

Keywords: Slovak Tsigai sheep; microsatellite markers; genetic relationship

Published: October 31, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kusza S, Gyarmathy E, Dubravska J, Nagy I, Jávor A, Kukovics S. Study of genetic differences among Slovak Tsigai populations using microsatellite markers. Czech J. Anim. Sci. 2009;54(10):468-474. doi: 10.17221/1670-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arranz J.J., Bayon Y., San Primitivo F. (1998): Genetic relationships among Spanish sheep using microsatellites. Animal Genetics, 29, 435-440. Go to original source... Go to PubMed...
    2. Arranz J.J., Bayon Y., San Primitivo F. (2001): Differentiation among Spanish sheep breeds using microsatellites. Genetic Selection Evolution, 33, 529-542. Go to original source... Go to PubMed...
    3. Benham J.J. (2001): Genographer, Ver. 1.6. Software can be obtained from http://hordeum.msu.montana.edu/genographer
    4. Calvo J.H., Bouzada J.A., Jurado J.J., Serrano M. (2006): Genetic substructure of the Spanish Manchega sheep breed. Small Ruminant Research, 64, 116-125. Go to original source...
    5. Ćinkulov M., Popovski Z., Porcu K., Tanaskovska B., Hodžić A., Bytyqi H., Mehmeti H., Margeta V., Djedović R., Hoda A., Trailović R., Brka M., Marković B., Važić B., Vegara M., Olsaker I., Kantanen J. (2008): Genetic diversity and structure of the West Balkan Pramenka sheep types as revealed by microsatellite and mitochondrial DNA analysis. Journal of Animal Breeding and Genetics, 125, 417-426. Go to original source... Go to PubMed...
    6. Dalvit C., Sacca E., Cassandro M., Gervaso M., Pastore E., Piasentier E. (2008): Genetic diversity and variability in Alpine sheep breeds. Small Ruminant Research, 80, 45-51. Go to original source...
    7. Farid A., O'Reilly E., Dollard C., Kelsey C.R. (2000): Genetic analysis of ten sheep breeds using microsatellite markers. Canadian Journal of Animal Science, 80, 9-17. Go to original source...
    8. Food and Agricultural Organization of United Nations 2001, 2004: www.fao.org/dad-is/
    9. Felsenstein J.: PHYLIP (Phylogeny Inference Package). Univ. Washington, USA, 1995.
    10. Gizaw S., Van Arendonk J.A., Komen H., Windig J.J., Hanotte O. (2007): Population structure, genetic variation and morphological diversity in indigenous sheep of Ethiopia. Animal Genetics, 38, 621-628. Go to original source... Go to PubMed...
    11. Gladyr E., Zinovieva N., Müller M., Brem G. (2005): Investigation of the Russian sheep breeds using DNA microsatellites. In: 56th Annual Meeting, European Association for Animal Production (EAAP), Uppsala, Sweden, 100 pp.
    12. Glowatzki-Mullis M.L., Muntwyler J., Bäumle E., Gaillard C. (2009): Genetic diversity of Swiss sheep breeds in the focus of conservation research. Journal of Animal Breeding and Genetics, 126, 164-175. Go to original source... Go to PubMed...
    13. Gyarmathy E. (2000): Tsigai sheep breeding in Slovakia. Personal Communication.
    14. Hedrick P.W. (1999): Highly variable loci and their interpretation in evolution and conservation. Evolution, 53, 313-314. Go to original source... Go to PubMed...
    15. Kukovics S., Jávor A. (2002): The Tsigai breed and its future. In: Jávor A., Mihók S. (eds.): Genepreservation, Research Result about the Values of Old Domestic Animals. Debrecen, Hungary, 103-147.
    16. Langella O. (1999): Populations. A software to calculate distances and create trees.
    17. Li X.L., Gong Y.F., Zhang J.W., Liu Z.Z., Valentini A.(2004): Study on polymorphism of microsatellites DNA of six Chinese indigenous sheep breeds. Yi Chuan Xue Bao, 31, 1203-1210. Go to PubMed...
    18. Mukesh M., Sodhi M., Bhatia S. (2006): Microsatellite based diversity analysis and genetic relationships of three Indian sheep breeds. Journal of Animal Breeding and Genetics, 123, 258-264. Go to original source... Go to PubMed...
    19. Nei M. (1987): Molecular Evolutionary Genetics. Columbia University Press, New York, USA. Go to original source...
    20. Ozerov M.Y, Marzanov N.S., Tapio M., Burabaev A.A., Marzanova L.K., Amerkhanov K.A., Kantanenen J. (2008): Genetic features of Kazakh sheep breeds according to microsatellites. Russian Agricultural Sciences, 34, 45-48. Go to original source...
    21. Peter C., Bruford M., Perez T., Dalamitra S., Hewitt G., Erhardt G., ECONOGENE Consortium (2007): Genetic diversity and subdivision of 57 European and MiddleEastern sheep breeds. Animal Genetics, 38, 37-44. Go to original source... Go to PubMed...
    22. Rooney A.P., Honeycutt R.L., Davis S.K., Derr J.N. (1999): Evaluating a putative bottleneck in a population of bowhead whales from patterns of microsatellite diversity and genetic disequilibria. Journal of Molecular Biology, 48, 682-690. Go to original source... Go to PubMed...
    23. Schandl J. (1955): Sheep breeding. Mezőgazdasági Kiadó, Budapest, Hungary. (in Hungarian)
    24. Schneider S., Roessli D., Excoffier L. (2000): Arlequin: A software for population genetic data. Genetics and Biometry Laboratory, University of Geneva, Geneva, Switzerland.
    25. Tapio M., Tapio I., Grislis Z., Holm L.E., Jeppson S., Kantanen J., Miceikiene I., Olsaker I., Viinalass H., Eythorsdottir E. (2005): Native breeds demonstrate high contributions to the molecular variation in Northern European sheep. Molecular Ecology, 14, 3951-3963. Go to original source... Go to PubMed...
    26. Weir B.S., Cockerham C.C. (1984): Estimating F-statistics for the analysis of population structure. Evolution, 38, 1358-1370. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content