Czech Journal of Animal Science, 2005 (vol. 50), issue 9
Breeding value for type traits in beef cattle in theCzech Republic
Z. Veselá, J. Přibyl, P. Šafus, L. Vostrý, K. Šeba, L. Štolc
Czech J. Anim. Sci., 2005, 50(9):385-393 | DOI: 10.17221/4223-CJAS 
The type was evaluated in 5 424 young animals of twelve beef breeds. Ten type traits were evaluated: height at sacrum (HS), body length (BL), live weight (LW), front chest width (CW), chest depth (CD), pelvis (P), shoulder muscling (SM), back muscling (BM), rump muscling (RM) and production type (PT). The traits represent two groups: (1) traits scoring body measurements and body capacity (HS, BL, LW, CW, CD, P) and (2) traits scoring muscling (SM, BM, RM, PT). These fixed effects were included in the model: breed, sex, HYS, mother's age, linear regression on age at evaluation and average gain from birth to evaluation. Fixed effects in the model explained...
Machine milking ability and milk composition of some imported dairy goat breeds and some crosses in Greece
J. Katanos, B. Skapetas, V. Laga
Czech J. Anim. Sci., 2005, 50(9):394-401 | DOI: 10.17221/4220-CJAS
A random sample of 40 goats was used to study the partitioning, yield and composition of milk: 8 of Saanen breed (S), 8 of Damascus breed (D), 8 crossbreds Saanen × Local Greek goat (S × L), 8 backcrosses Saanen × Local Greek goat {(S × L) × S} and 8 crossbreds Saanen × Alpine (S × A). Four dams of each genotype were of the first and four of the second parity. Goats were milked twice daily (8:00, 18:00 h) in a 1 × 12 side by side milking parlour with 6 milking units. Milk yield and milk fractions (machine milk, machine stripped milk and hand stripped milk) were recorded twice daily each 14 days (from 7th-8th to 29th...
Comparison of apparent and true digestibility of nutrients determined in dairy cows either by the nylon capsule or in vivo method
J. Třináctý, M. Richter, P. Homolka, M. Rabišková, P. Doležal
Czech J. Anim. Sci., 2005, 50(9):402-410 | DOI: 10.17221/4181-CJAS
In this study the values of true digestibility of DM, OM, CP, NDF and starch determined either by the nylon capsule method or conventional in vivo method were compared. Four intact crossbred dairy cows (mean milk yield 21.9 kg/d) were used in two experimental periods. TMR consisted of maize silage, lucerne hay and concentrate. Nylon capsules (external diameter 10 mm) were made of nylon cloth (pore size 42 µm). Capsules were filled with TMR and at the beginning of the in vivo trial they were administered orally as a paper bolus into the cows. The values of true digestibility (after washing loss correction) of DM and OM, as determined...
Effect of age on digestibility of starch in chickens with different growth rate
J. Zelenka, Z. Čerešňáková
Czech J. Anim. Sci., 2005, 50(9):411-415 | DOI: 10.17221/4222-CJAS
Digestibility of starch was examined in slow-growing cockerels of laying type (SG) and in fast-growing male chickens (FG) of broiler type fed ad libitum on a maize-type diet until Day 22 of age in one-day periods and from Day 22 to Day 100 in three-day periods. Digestibility was estimated using the chromic oxide indicator method. In SG chicks, starch digestibility rapidly increased within the first days of their lives and reached the value of 0.986 already on Day 4 of life. It was maintained on this level till the end of the experiment, which was finished at the age of 100 days. In FG chicks, which in comparison with SG birds consumed more...
Enterococci from rabbits - potential feed additive
M. Simonová, A. Lauková, I. Štyriak
Czech J. Anim. Sci., 2005, 50(9):416-421 | DOI: 10.17221/4221-CJAS
Enterococci (58) from faeces of rabbits of various age (from 2 months to 3 years) and 5 different rabbit farms were isolated and tested for survival in the presence of oxgall, lactic acid production, urease activity, resistance to low pH as well as their binding ability was tested. Fifty percent of enterococcal isolates were identified as Enterococcus faecium, 19% as E. faecalis. All strains showed good survival in the presence of 5% oxgall. The urease activity of isolates was in the range from 0.013 to 17.13 nkat/ml, only E. faecalis EE229 strain did not produce any urease. The survival of strains was tested at pH 3.0 and...
Cathepsin involvement in muscle proteolysis in meat-type bulls
S.J. Rosochacki, T. Sakowski, E. Juszczuk-Kubiak, A. Butarewicz, J. Połoszynowicz
Czech J. Anim. Sci., 2005, 50(9):422-429 | DOI: 10.17221/4226-CJAS
Measurements were done of some lysosomal proteolytic enzyme activities involved in skeletal muscle proteolysis of the masculus longissimus lumborum et thoracis muscle (MLLT) of bulls. Samples from the same region between the 11th and 13th vertebra were taken after slaughter from Limousin (n = 10), Hereford (n = 10), Charolais (n = 10), Angus (n = 11) and Simmental (n = 11) bulls about 15 months old fed complete diet ad libitum. The activity of cathepsin D was determined as pepstatin (cathepsin D inhibitor) sensitive activity (PSCatD) towards 1% haemoglobin. Pepstatin-insensitive...
Separation and identification of carp pituitary proteins and glycoproteins
H. Ryšlavá, M. Janatová, G. Čalounová, I. Selicharová, J. Barthová, T. Barth
Czech J. Anim. Sci., 2005, 50(9):430-437 | DOI: 10.17221/4232-CJAS
Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide...