Czech J. Anim. Sci., 2005, 50(9):430-437 | DOI: 10.17221/4232-CJAS

Separation and identification of carp pituitary proteins and glycoproteins

H. Ryšlavá1, M. Janatová1, G. Čalounová1, I. Selicharová2, J. Barthová1, T. Barth2
1 Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech Republic
2 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO4-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)2(Man)3, were found.

Keywords: carp hormones; glycoproteins; immobilized metal ion affinity chromatography (IMAC); oligosaccharide chains

Published: September 30, 2005  Show citation

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Ryšlavá H, Janatová M, Čalounová G, Selicharová I, Barthová J, Barth T. Separation and identification of carp pituitary proteins and glycoproteins. Czech J. Anim. Sci. 2005;50(9):430-437. doi: 10.17221/4232-CJAS.
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