Czech J. Anim. Sci., 2005, 50(9):416-421 | DOI: 10.17221/4221-CJAS

Enterococci from rabbits - potential feed additive

M. Simonová, A. Lauková, I. Štyriak
Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic

Enterococci (58) from faeces of rabbits of various age (from 2 months to 3 years) and 5 different rabbit farms were isolated and tested for survival in the presence of oxgall, lactic acid production, urease activity, resistance to low pH as well as their binding ability was tested. Fifty percent of enterococcal isolates were identified as Enterococcus faecium, 19% as E. faecalis. All strains showed good survival in the presence of 5% oxgall. The urease activity of isolates was in the range from 0.013 to 17.13 nkat/ml, only E. faecalis EE229 strain did not produce any urease. The survival of strains was tested at pH 3.0 and the percentage of their survival ranged between 62.0% and 90.0%. E. faecium EF1819 strain was found to show the best survival ability at low pH. Particle agglutination assay values of selected enterococci expressed only negative (0) or weakly positive (1) binding of heparin, bovine fibrinogen, porcine fibronectin and lactoferrin. Based on the results, most of the selected enterococci could be promising probiotic feed additives.

Keywords: probiotic feed additive; rabbit; enterococci; properties

Published: September 30, 2005  Show citation

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Simonová M, Lauková A, Štyriak I. Enterococci from rabbits - potential feed additive. Czech J. Anim. Sci. 2005;50(9):416-421. doi: 10.17221/4221-CJAS.
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References

  1. Bomba A., Kašteľ R., Gancarčíková S., Nemcová R., Herich R., Čížek M. (1996): The effect of lactobacilli inoculation on organic acid levels in the mucosal film and the small intestine contents in gnotobiotic pigs. Berl. Münch. Tierärztl. Wschr., 9, 428-430.
  2. Cook A.R. (1976): Urease activity in the rumen of sheep and the isolation of ureolytic bacteria. J. Gen. Microbiol., 92, 32-48. Go to original source... Go to PubMed...
  3. DeVriese L.A., Hommez J., Wijfels R., Halsebrouck E. (1991): Composition of the enterococcal and streptococcal intestinal flora of poultry. J. Appl. Bacteriol., 71, 46-50. Go to original source... Go to PubMed...
  4. Fuller R. (1989): Probiotics in man and animals: A review. J. Appl. Bacteriol., 66, 365-378. Go to original source... Go to PubMed...
  5. Gidenne T. (1997): Caeco-colic digestion in the growing rabbit: impact of nutritional factors and related disturbances. Livest. Prod. Sci., 51, 73-88. Go to original source...
  6. Gilliland S.E., Walker D.K. (1990): Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci., 73, 905-911. Go to original source... Go to PubMed...
  7. Gouet P., Fonty G. (1979): Changes in the digestive microflora of the holoxenic rabbits from birth until adulthood. Ann. Biol. Biochem. Biophys., 19, 553-566. Go to original source...
  8. Hausinger R.P. (1986): Purification of a nickel-containing urease from the rumen anaerobic Selenomonas ruminantium. J. Biol. Chem., 17, 7866-7870. Go to original source...
  9. Lauková A., Guba P., Nemcová R., Vasilková Z. (2003): Reduction of Salmonella in gnotobiotic Japanese quails caused by the Enterocin A-producing EK13 strain of Enterococcus faecium. Vet. Res. Com., 27, 275-280.
  10. Lauková A., Juriš P. (1997): Distribution and characterization of Enterococcus species in municipal sewages. Microbios, 89, 73-80. Go to PubMed...
  11. Lauková A., Mareková M., Javorský P. (1993): Detection an antimicrobial spectrum of a bacteriocin like substance produced by Enterococcus faecium CCM4231. Appl. Microbiol., 16, 257-260. Go to original source...
  12. Linaje R., Coloma M.D., Pérez-Martínez G., Zúñiga M. (2004): Characterization of faecal enterococci from rabbits for the selection of probiotic strains. J. Appl. Microbiol., 96, 761-771. Go to original source... Go to PubMed...
  13. Marciňáková M., Strompfová V., Boldižárová K., Lauková A., Gancarčíková S. (2004): Effect of potential probiotic activity of Enterococcus faecium EE3 strain against Salmonella infection in Japanese quails. Bull. Vet. Inst. Pulawy, 48, 387-390.
  14. Naidu A.S., Paulsson M., Wadström T. (1998): Particle agglutination assays for rapid detection of fibronectin, fibrinogen, and collagen receptors on Staphylococcus aureus. J. Clin. Microbiol., 26, 1549-1554. Go to original source... Go to PubMed...
  15. Nemcová R. (1997): Criteria for selection of lactobacilli for probiotic use. Vet. Med., 42, 19-27.
  16. Salminen S., Von Wright A., Morelli L., Martenau P., Brassart D., de Vos W.M., Fondeu R., Saxelin M., Collins K., Mogensen G., Birkeland S.-E., Mattila-Sandholm T. (1998): Demonstration of safety of probiotics - a review, Int. J. Food Microbiol., 44, 93-106. Go to original source... Go to PubMed...
  17. Schryvers A.B., Gonzales G.C. (1989): Comparison of the abilities of different protein sources of iron to enhance Neisseria meningitidis infection in mice. Infect. Immun., 57, 2425-2429. Go to original source... Go to PubMed...
  18. Simon O., Jadamus A., Vahjenen W. (2001): Probiotic feed additives - effectiveness and expected modes of action. J. Anim. Feed Sci., 10, 51-67. Go to original source...
  19. Simonová M., Lauková A. (2004a): Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production. Bull. Vet. Inst. Pulawy, 48, 383-386.
  20. Simonová M., Lauková A. (2004b): Lactic acid bacteria in rabbit's ecosystem. In: Proceedings of International Probiotic Conference, New Perspectives of Probiotics, Košice, Slovak Republic. 40 pp.
  21. Strompfová V., Lauková A., Ouwehand A.C. (2004): Selection of enterococci for potential canine probiotic additives. Vet. Microbiol., 100, 107-114. Go to original source... Go to PubMed...
  22. Štyriak I., Lauková A., Fallgreu C., Wadström T. (1999): Binding of selected extracellular matrix proteins to enterococci and Streptococcus bovis of animal origin. Current Microbiol., 39, 327-335. Go to original source... Go to PubMed...
  23. Štyriak I., Lauková A., Strompfová V., Ljungh A. (2004): Mode of binding of fibrinogen, fibronectin and ironbinding proteins by animal enterococci. Vet. Res. Com., 28, 587-598. Go to original source... Go to PubMed...
  24. Štyriak I., Nemcová R., Chang Y.-H., Ljungh A. (2003): Binding of extracellular matrix molecules by probiotic bacteria. Lett. Appl. Microbiol., 37, 329-333. Go to original source... Go to PubMed...
  25. Woodford N., Egelton M.C., Morrison D. (1997): Comparison of PCR with phenotypic methods for the speciation of enterococci. Plenum Press, New York, 47, 405-409. Go to original source... Go to PubMed...
  26. Yu B., Tsen H. (1993): Lactobacillus cells in the rabbit digestive-tract and the factors affecting their distribution. J. Appl. Bacteriol., 75, 269-275. Go to original source... Go to PubMed...

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