Czech J. Anim. Sci., 2022, 67(4):137-146 | DOI: 10.17221/61/2021-CJAS

Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughnessOriginal Paper

Yuchun Xie ORCID...#,1,2,3, Nai Rile ORCID...#,4, Xuewu Li5, Haijun Li6, Meng Zhao7, Tianyu Che ORCID...1,2,3, Ting Cai8, Zhihong Liu*,1,2,3, Jinquan Li*,1,2,3
1 College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
2 Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, P.R. China
3 Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, P.R. China
4 School of Agriculture and Forestry, Hulunbuir University, Hulunbuir, P.R. China
5 School of Health and Wellness, Panzhihua University, Panzhihua, P.R. China
6 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, P.R. China
7 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China
8 Inner Mongolia Autonomous Region Agriculture and Animal Husbandry Technology Extension Center, Hohhot, P.R. China

The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed's meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein-protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.

Keywords: SWATH; interaction network; muscle fibre type

Published: April 30, 2022  Show citation

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Xie Y, Rile N, Li X, Li H, Zhao M, Che T, et al.. Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness. Czech J. Anim. Sci. 2022;67(4):137-146. doi: 10.17221/61/2021-CJAS.
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