Czech J. Anim. Sci., 2019, 64(8):325-331 | DOI: 10.17221/66/2019-CJAS

In vitro antimicrobial effect of palm oils rich in medium-chain fatty acids against mastitis-causing Gram-positive bacteriaOriginal Paper

Klára Laloučková1,2, Lucie Malá1,2, Paula Slaničková2, Eva Skřivanová*,1,2
1 Department of Nutritional Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic
2 Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic

Various pathogens causing mastitis in dairy cattle are of serious concern due to their increasing antibacterial resistance and potential transmission to other cows, calves, and the environment, especially through the milking process. Therefore, alternative approaches to antimicrobial usage in the treatment or control of mastitis in dairy cattle are severely needed. The antibacterial effect of medium-chain fatty acids (MCFAs) is known to be significant for various pathogens, but there is only limited information about the activity of MCFAs on mastitis-causing pathogens. Moreover, no evidence about the antimicrobial effects of palm oils rich in MCFAs, such as coconut, palm kernel, and tucuma oil, can be found in the current literature. The aim of this study was to evaluate the in vitro antibacterial effect of palm oils rich in MCFAs, after cleavage by an exogenous lipase from Mucor javanicus, on bovine mastitis-causing strains (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis) by the broth microdilution method. All tested palm oils exerted antibacterial activity against eight tested bacterial strains in the range of 64-8192 µl/ml with Str. agalactiae being the most sensitive and S. aureus being the most resistant species. The results of the present study demonstrate that palm oils rich in MCFAs can serve as an alternative to the predominantly used predip and postdip procedures in bovine mastitis control, but further in vivo studies are needed to confirm the findings for their possible applications.

Keywords: antibacterial; bovine; Staphylococcus; Streptococcus; vegetable oil

Published: August 31, 2019  Show citation

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Laloučková K, Malá L, Slaničková P, Skřivanová E. In vitro antimicrobial effect of palm oils rich in medium-chain fatty acids against mastitis-causing Gram-positive bacteria. Czech J. Anim. Sci. 2019;64(8):325-331. doi: 10.17221/66/2019-CJAS.
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    References

    1. Barkema H.W., Schukken Y.H., Zadoks R.N. (2006): Invited review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. Journal of Dairy Science, 89, 1877-1895. Go to original source... Go to PubMed...
    2. Baselga R., Albizu I., Amorena B. (1994): Staphylococcus aureus capsule and slime as virulence factors in ruminant mastitis. A review. Veterinary Microbiology, 39, 195-204. Go to original source... Go to PubMed...
    3. Batovska D.I., Todorova T., Tsvetkova V., Najdenski H.M. (2009): Antibacterial study of the medium chain fatty acids and their 1-monoglycerides: Individual effects and synergistic relationships. Polish Journal of Microbiology, 58, 43-47.
    4. Behiry A.E., Schlenker G., Szabo I., Roesler U. (2012): In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents. Journal of Veterinary Science, 13, 153-161. Go to original source... Go to PubMed...
    5. Breen J. (2017): The importance of non-steroidal antiinflammatory drugs (NSAIDs) in mastitis therapeutics. Livestock, 4, 182-185. Go to original source...
    6. Bunkova L., Bunka F., Janis R., Krejci J., Dolezalkova I., Pospisil Z., Ruzicka J., Tremlova B. (2011): Comparison of antibacterial effect of seven 1-monoglycerides on foodborne pathogens or spoilage bacteria. Acta Veterinaria Brno, 80, 29-39. Go to original source...
    7. Chamberlain N.R., Mehrtens B.G., Xiong Z., Kapral F.A., Boardman J.L., Rearick J.I. (1991): Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z. Infection and Immunity, 59, 4332-4337. Go to original source... Go to PubMed...
    8. Churchward C.P., Alany R.G., Snyder L.A. (2018): Alternative antimicrobials: The properties of fatty acids and monoglycerides. Critical Reviews in Microbiology, 44, 561-570. Go to original source... Go to PubMed...
    9. CLSI (2013): Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Approved Standard M07-A10. Clinical and Laboratory Standards Institute, Wayne, USA.
    10. Erskine R., Cullor J., Schaellibaum M., Yancey B., Zecconi A. (2004): Bovine mastitis pathogens and trends in resistance to antibacterial drugs. In: Proc. National Mastitis Council (NMC) Annual Meeting, Verona, Italy, 400-414.
    11. FDA (2019): Electronic Code of Federal Regulations. U.S. Food and Drug Administration, College Park, USA. Available at https://www.ecfr.gov/cgi-bin/text-idx?SID=3ee286332416f26a91d9e6d786a604ab&mc=true&tpl=/ecfrbrowse/Title21/21tab_02.tpl (accessed Feb 25, 2019).
    12. Gomes F., Henriques M. (2016): Control of bovine mastitis: Old and recent therapeutic approaches. Current Microbiology, 72, 377-382. Go to original source... Go to PubMed...
    13. Hossain M.K., Paul S., Hossain M.M., Islam M.R., Alam M.G.S. (2017): Bovine mastitis and its therapeutic strategy doing antibiotic sensitivity test. Austin Journal of Veterinary Science and Animal Husbandry, 4, 1030. Go to original source...
    14. Hovorkova P., Lalouckova K., Skrivanova E. (2018): Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria. Czech Journal of Animal Science, 63, 119-125. Go to original source...
    15. Jingar S.C., Singh M., Roy A.K. (2017): Economic losses due to clinical mastitis in cross-bred cows. International Journal of Livestock Research, 8, 254-263.
    16. Kasna E., Zavadilova L., Stipkova M. (2018): Genetic evaluation of clinical mastitis traits in Holstein cattle. Czech Journal of Animal Science, 63, 443-451. Go to original source...
    17. Kromker V., Grabowski N.T., Friedrich J. (2014): New infection rate of bovine mammary glands after application of an internal teat seal at dry-off. Journal of Dairy Research, 81, 54-58. Go to original source... Go to PubMed...
    18. Legrand P. (2008): Nutritional interest of different fatty acids from milk fat. Sciences des Aliments, 28, 34-43. Go to original source...
    19. Mushtaq S., Shah A.M., Shah A., Lone S.A., Hussain A., Hassan Q.P., Ali M.N. (2018): Bovine mastitis: An appraisal of its alternative herbal cure. Microbial Pathogenesis, 114, 357-361. Go to original source... Go to PubMed...
    20. Nair M.K.M., Joy J., Vasudevan P., Hinckley L., Hoagland T.A., Venkitanarayanan K.S. (2005): Antibacterial effect of caprylic acid and monocaprylin on major bacterial mastitis pathogens. Journal of Dairy Science, 88, 3488-3495. Go to original source... Go to PubMed...
    21. Oyedeji F.O., Okeke I.E. (2010): Comparative analysis of moisturizing creams from vegetable oils and paraffin oil. Research Journal of Applied Sciences, 5, 157-160. Go to original source...
    22. Polycarpo G.V., Andretta I., Kipper M., Cruz-Polycarpo V.C., Dadalt J.C., Rodrigues P.H.M., Albuquerque R. (2017): Meta-analytic study of organic acids as an alternative performance-enhancing feed additive to antibiotics for broiler chickens. Poultry Science, 96, 3645-3653. Go to original source... Go to PubMed...
    23. Schlievert P.M., Peterson M.L. (2012): Glycerol monolaurate antibacterial activity in broth and biofilm cultures. PLoS ONE, 7, e40350. Go to original source... Go to PubMed...
    24. Schreiner D.A., Ruegg P.L. (2003): Relationship between udder and leg hygiene scores and subclinical mastitis. Journal of Dairy Science, 86, 3460-3465. Go to original source... Go to PubMed...
    25. Srivastava Y., Semwal A.D., Majumdar A. (2016): Quantitative and qualitative analysis of bioactive components present in virgin coconut oil. Cogent Food and Agriculture, 2, Article 1164929. Go to original source...
    26. Ster C., Allard M., Boulanger S., Boulet M.L., Mulhbacher J. (2013): Experimental treatment of Staphylococcus aureus bovine intramammary infection using a guanine riboswitch ligand analog. Journal of Dairy Science, 96, 1000-1008. Go to original source... Go to PubMed...
    27. Van Boeckel T.P., Brower C., Gilbert M., Grenfell B.T., Levin S.A., Robinson T.P., Teillant A., Laxminarayan R. (2015): Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences of the United States of America, 112, 5649-5654. Go to original source... Go to PubMed...
    28. Van der Vossen H.A.M., Umali B.E., Oyen L.P.A., Jansen P.C.M. (2001): Vegetable oils and fats. In: Jansen P.C.M., Westphal E., Wulijarni-Soetjipto N. (eds): Plant Resources of South-East Asia, Volume 14. Backhuys Publishers, Leiden, the Netherlands, 13-170.
    29. Wadhwani T., Desai K., Patel D., Lawani D., Bahaley P., Joshi P., Kothari V. (2009): Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials. The Internet Journal of Microbiology, 7, 1-13. Go to original source...

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