Czech J. Anim. Sci., 2013, 58(10):443-452 | DOI: 10.17221/6993-CJAS

Effect of various co-culture systems on embryo development in ovineOriginal Paper

B. Heidari1, A. Shirazi1,2, M.-M. Naderi1, M.-M. Akhondi1, H. Hassanpour2, A. Sarvari1, S. Borjian1
1 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2 Department of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran

Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey's test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups.

Keywords: mesenchymal stem cell; fibroblast; oviduct; in vitro production; zygote

Published: October 31, 2013  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Heidari B, Shirazi A, Naderi M-M, Akhondi M-M, Hassanpour H, Sarvari A, Borjian S. Effect of various co-culture systems on embryo development in ovine. Czech J. Anim. Sci. 2013;58(10):443-452. doi: 10.17221/6993-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bavister B.D. (1992): Co-culture for embryo development: Is it really necessary? Human Reproduction, 7, 1339-1341. Go to original source... Go to PubMed...
    2. Bernardi M.L., Flechon J.E., Delouis C. (1996): Influence of culture system and oxygen tension on the development of ovine zygotes matured and fertilized in vitro. Journal of Reproduction and Fertility, 106, 161-167. Go to original source... Go to PubMed...
    3. Biggers J.D., Gwatkin R.B., Brinster R.L. (1962): Development of mouse embryos in organ cultures of fallopian tubes on a chemically defined medium. Nauchni Trudove na Visshiia Meditsinski Institut, Sofiia, 194, 747-749. Go to original source... Go to PubMed...
    4. Bongso A., Soon-Chye N., Sathananthan H., Lian N.P., Rauff M., Ratnam S. (1989): Improved quality of human embryos when co-cultured with human ampullary cells. Human Reproduction, 4, 706-713. Go to original source... Go to PubMed...
    5. Cremonesi F., Maggio V., Lange-Consiglio A. (2008): Equine amniotic epithelial or bone marrow mesenchymal stem cells differently support in vitro embryo development in a bovine in vitro culture model. Reproduction, Fertility and Development, 21, 156. Go to original source...
    6. Desai N.N., Goldfarb J.M. (1998): Co-cultured human embryos may be subjected to widely different microenvironments: pattern of growth factor/cytokine release by Vero cells during the co-culture interval. Human Reproduction, 13, 1600-1605. Go to original source... Go to PubMed...
    7. Ellington J.E., Carney E.W., Farrell P.B., Simkin M.E., Foote R.H. (1990): Bovine 1-2-cell embryo development using a simple medium in three oviduct epithelial cell coculture systems. Biology of Reproduction, 43, 97-104. Go to original source... Go to PubMed...
    8. Freshney I.R. (1994): Culture of Animal Cells. 3rd Ed. WileyLiss, New York, USA.
    9. Galli C., Duchi R., Crotti G., Turini P., Ponderato N., Colleoni S., Lagutina I., Lazzari G. (2003): Bovine embryo technologies. Theriogenology, 59, 599-616. Go to original source... Go to PubMed...
    10. Gandolfi F., Moor R.M. (1987): Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. Journal of Reproduction and Fertility, 81, 23-28. Go to original source... Go to PubMed...
    11. Hu Y., Maxson W., Hoffman D., Ory S., Eager S., Dupre J., Worrilow K. (1998): Co-culture with assisted hatching of human embryos using Buffalo rat liver cells. Human Reproduction, 13, 165-168. Go to original source... Go to PubMed...
    12. Joo B.S., Kim M.K., Na Y.J., Moon H.S., Lee K.S., Kim H.D.K. (2001): The mechanism of action of coculture on embryo development in the mouse model: direct embryo-to-cell contact and the removal of deleterious components. Fertility and Sterility, 75, 193-199. Go to original source... Go to PubMed...
    13. Kervancioglu M.E., Saridogan E., Atasü T., Camlibel T., Demircan A., Sarikamis B., Djahanbakhch O. (1997): Human Fallopian tube epithelial cell co-culture increases fertilization rates in male factor infertility but not in tubal or unexplained infertility. Human Reproduction, 12, 1253-1258. Go to original source... Go to PubMed...
    14. Leese H.J. (1988): The formation and function of oviduct fluid. Journal of Reproduction and Fertility, 82, 843-856. Go to original source... Go to PubMed...
    15. Ling B., Feng D., Zhou Y., Gao T., Wei H., Tian Z. (2008): Effect of conditioned medium of mesenchymal stem cells on the in vitro maturation and subsequent development of mouse oocyte. Brazilian Journal of Medical and Biological Research, 41, 978-985. Go to original source... Go to PubMed...
    16. Marcus S.F., Brinsden P.R. (1996): In-vitro fertilization and embryo transfer in women aged 40 years and over. Human Reproduction, 2, 459-468. Go to original source... Go to PubMed...
    17. Mohamed E.S., Osama M.A., Dina-Sabry A.F., Tamer F. (2011): Stem cell supernatant fluid as a co-culture medium may improve poor embryo quality. Evidence Based Women's Health Journal, 1, 8-10.
    18. Nematollahi-Mahani S.N., Pahang H., Moshkdanian G., Nematollahi-Mahani A.M. (2009): Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light. Journal of Assisted Reproduction and Genetics, 26, 129-135. Go to original source... Go to PubMed...
    19. Orsi N.M., Reischl J.B. (2007): Mammalian embryo co-culture: trials and tribulations of a misunderstood method. Theriogenology, 67, 441-458. Go to original source... Go to PubMed...
    20. Park K.-S., Kim Y.-S., Kim J.-H., Choi B., Kim S.-H., Tan A.H.-K., Lee M.-S., Lee M.-K., Kwon Ch.-H., Joh J.-W., Kim S.-J., Kim K.-W. (2010): Trophic molecules derived from human mesenchymal stem cells enhance survival, function, and angiogenesis of isolated islets after transplantation. Transplantation, 15, 509-517. Go to original source...
    21. Rief S., Sinowatz F., Stojkovic M., Einspanier R., Wolf E., Prelle K. (2002): Effects of a novel co-culture system on development, metabolism and gene expression of bovine embryos produced in vitro. Reproduction, 124, 543-556. Go to original source... Go to PubMed...
    22. Rodriguez-Boulan E., Nelson W.J. (1989): Morphogenesis of the polarized epithelial cell phenotype. Science, 245, 718-725. Go to original source... Go to PubMed...
    23. Smith S., Schmidt M., Purwantara B., Greve T. (1992): Oviduct epithelial cell co-culture of early porcine embryos. Acta Veterinaria Scandinavica, 33, 349-355. Go to original source... Go to PubMed...
    24. Shamsuddin M., Rodriguez-Martinez H. (1994): Fine structure of bovine blastocysts developed either in serum-free medium or in conventional co-culture with oviduct epithelial cells. Journal of Veterinary Medicine, A, 41, 307-316. Go to original source... Go to PubMed...
    25. Teklenburg G., Macklon N.S. (2009): Review: In vitro models for the study of early human embryo-endometrium interactions. Reproductive Sciences, 16, 811-818. Go to original source... Go to PubMed...
    26. Tian L.L., Yue W., Zhu F., Li S., Li W. (2011): Human mesenchymal stem cells play a dual role on tumor cell growth in vitro and in vivo. Journal of Cellular Physiology, 226, 1860-1867. Go to original source... Go to PubMed...
    27. Tucker M.J., Kort H.I., Toledo A.A., Morton P.C., Wrigh G., Ingargiola P.E., Sweitzer C.L. (1995): Effect of coculture on subsequent survival and implantation of cryopreserved human embryos. Journal of Assisted Reproduction and Genetics, 12, 689-692. Go to original source... Go to PubMed...
    28. Wetzels A.M.M., Bastiaans B.A., Hendriks J.C.M., Goverde H.J.M., van der Zalm A.P.E.M.P., Verbeet J.G.M., Braat D.D.M. (1998): The effects of co-culture with human fibroblasts on human embryo development in vitro and implantation. Human Reproduction, 13, 1325-1330. Go to original source... Go to PubMed...
    29. Wiemer K.E., Cohen J., Wiker S.R., Malter H.E., Wright G., Godke R.A. (1989): Coculture of human zygotes on fetal bovine uterine fibroblasts: embryonic morphology and implantation. Fertility and Sterility, 52, 503-508. Go to original source... Go to PubMed...
    30. Xu J., Cheung T.M., Chan S.T., Ho P.C., Yeung W.S. (2000): Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos. Fertility and Sterility, 74, 1215-1219. Go to original source... Go to PubMed...
    31. Yeung W.S., Ho P.C., Lau E.Y., Chan S.T. (1992): Improved development of human embryos in vitro by a human oviductal cell co-culture system. Human Reproduction, 7, 1144-1149. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content