Czech J. Anim. Sci., 2013, 58(6):262-269 | DOI: 10.17221/6824-CJAS

Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattleOriginal Paper

E. Juszczuk-Kubiak1, K. Wicińska1, J. Oprządek2
1 Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland
2 Deparment of Animal Sciences, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland

In the present study the polymorphism of the myocyte enhancer factor 2D (MEF2D) gene was analyzed as a genetic marker candidate for carcass traits in Polish Holstein-Friesian cattle. Four novel single nucleotide polymorphisms (SNPs): HQ692911:g.93C>T in exon 8, JX088659:g.69C>T in intron 8, JX0692914:g.47C>T in intron 9 as well as JX088658:g.100G>A in the 3'flanking region were identified by DNA sequencing. By applying the PCR-RFLP method, the SNPs (g.93C>T/HaeIII and g.47C>T/Tsp45I) were genotyped in 401 Polish Holstein-Friesian bulls and their association with carcass traits was analyzed. Statistical analysis showed that the g.93C>T SNP was significantly associated with weight of lean (WLVC) and fat in valuable cuts (WFVC). Animals with the TT genotype had significantly higher WLVC (P < 0.05) and lower WFVC (P < 0.05) than those with the CC and CT genotypes. No significant relationship with carcass traits was found for the g.47C>T SNP (P > 0.05) in intron 9. Moreover, we found a significant effect of the combined genotypes onWFVC (P < 0.01), percent of lean (PLVC) (P < 0.05), and fat (PFVC) in valuable cuts (P < 0.01). This suggested that MEF2D is a strong candidate gene that affects carcass traits in cattle.

Keywords: MEF2D gene; gene polymorphism; combined genotype; meat contents; bulls

Published: June 30, 2013  Show citation

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Juszczuk-Kubiak E, Wicińska K, Oprządek J. Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle. Czech J. Anim. Sci. 2013;58(6):262-269. doi: 10.17221/6824-CJAS.
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