Czech J. Anim. Sci., 2011, 56(11):475-482 | DOI: 10.17221/3836-CJAS

Analysis of polymorphism in the porcine TLR4 gene and its expression related to Escherichia coliF18 infection

W.B. Bao1,3, L. Ye1, Z.Y. Pan1, J. Zhu1, G.Q. Zhu2,3, X.G. Huang3, S.L. Wu1,3
1 Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China
2 College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China
3 Jiangsu Engineering Research Centre for Molecular Breeding of Pig, Changzhou, P.R. China

The genetic variation in exon 1 of the TLR4 gene was detected among a total of 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous pig breeds. The expression of TLR4 was assayed by RT-PCR and different expression between resistant and sensitive resource populations to ETEC F18 was analysed to discuss the role that the TLR4 gene plays in resistance. In this study, new alleles were detected in exon 1 of the TLR4 gene. These polymorphisms are significantly different between Chinese indigenous breeds and imported breeds. Based on the published TLR4 gene sequence (AB232527) in GenBank, a 93G > C mutation was found in 5'UTR and only a 194G > A synonymous mutation was found in the coding sequence of exon 1. In addition, TLR4 gene was broadly expressed in 11 tissues with the highest level in lung. The expression was relatively high in the lymph nodes, kidney and spleen. Generally, the expression of TLR4 gene in sensitive individuals was higher than that in resistant individuals. The results indicated that the downregulation of the mRNA expression of TLR4 gene had reduced the transmembrane signal transduction of LPS and then led to the responsive ability of the host to ETEC F18 in piglets.

Keywords: pigs; TLR4 gene; polymorphism; expression profile; ETEC F1

Published: November 30, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bao WB, Ye L, Pan ZY, Zhu J, Zhu GQ, Huang XG, Wu SL. Analysis of polymorphism in the porcine TLR4 gene and its expression related to Escherichia coliF18 infection. Czech J. Anim. Sci. 2011;56(11):475-482. doi: 10.17221/3836-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bao W.B., Wu S.L., Musa H.H., Zhu G.Q., Chen G.H. (2008): Genetic variation at the alpha (1) fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds. Journal of Animal Breeding and Genetics, 125, 427-430. Go to original source... Go to PubMed...
    2. Benin A.M., Ducher-Suchaux M.F. (1991): Relationship between virulence and adherence of various enterotoxigenic Escherichia coli strains to isolated intestinal epithelial cells from Chinese Meishan and European large white pigs. American Journal of Veterinary Research, 52, 45-49. Go to original source...
    3. Beutler B. (2005): The Toll-like receptors: analysis by forward genetic methods. Immunogenetics, 57, 385-392. Go to original source... Go to PubMed...
    4. Giorgio T., Alan S. (2007): Cooperation of Toll-like receptor signals in innate immune defence. Nature Reviews Immunology, 7, 179-190. Go to original source... Go to PubMed...
    5. Goldammer T., Zerbe H., Molenaar A., Schuberth H.J., Brunner R.M., Kata S.R., Seyfert H.M. (2004): Mastifs increases mammary mRNA abundance of beta-defensin 5, toll like-receptor 2 (TLR2) and TLR4 but not TLR9 in cattle. Clinical and Diagnostic Laboratory Immunology, 11, 174-185. Go to original source... Go to PubMed...
    6. Hirschfeld M., Ma Y., Weis J.H., Vogel S.N., Weis J.J. (2000): Repurification of LPS eliminates signaling through both human and murine Toll-like receptor 2. The Journal of Immunology, 165, 618-622. Go to original source... Go to PubMed...
    7. Lazarus R., Vercelli D., Palmer L.J., Klimecki W.J., Silverman E.K., Richter B., Riva A., Ramoni M., Martinez F.D., Weiss S.T., Kwiatkowski D.J. (2002): Single nucleotide polymorphisms in innate immunity genes: abundant variation and potential role in complex human disease. Immunological Reviews, 190, 9-25. Go to original source... Go to PubMed...
    8. Leveque G., Forgetta V., Morroll S., Smith A.L., Bumstead N., Barrow P., Loredo-Osti J.C., Morgan K., Malo D. (2003): Allelic variation in TLR4 is linked to susceptibility to Salmonella enterica serovar Typhimurium infection in chickens. Infection and Immunity, 71, 1116-1124. Go to original source... Go to PubMed...
    9. Machida K., Cheng K.T., Sung V.M., Levine A.M., Foung S., Lai M.M. (2006): Hepatitis C virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon and interleukin-6. Journal of Virology, 80, 866-874. Go to original source... Go to PubMed...
    10. Medzhitov R. (2001): Toll-like receptors and innate immunity. Nature Reviews Immunology, 1, 135-145. Go to original source... Go to PubMed...
    11. Meijerink E., Fries R., Vogeli P., Masabanda J., Wigger G., Stricker C., Neuenschwander S., Bertschinger H.U., Stranzinger G. (1997): Two α(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci. Mammalian Genome, 8, 736-741. Go to original source... Go to PubMed...
    12. Nagy B., Fekete P.Z. (1999): Enterotoxigenic Escherichia coli (ETEC) in farm animal. Veterinary Research, 30, 259-284.
    13. Ozinsky A., Underhill D.M., Fontenot J.D., Hajjar A.M., Smith K.D., Wilson C.B., Schroeder L., Aderem A. (2000): The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between Toll-like receptors. Proceedings of the National Academy of Sciences of the United States of America, 97, 13766-13771. Go to original source... Go to PubMed...
    14. Palermo S., Capra E., Torremorell M., Dolzan M., Davoli R., Haley C.S., Giuffra E. (2009): Toll-like receptor 4 genetic diversity among pig populations. Animal Genetics, 2009, 40, 289-299. Go to original source... Go to PubMed...
    15. Poltorak A., Ricciardi-Castagnoli P., Citterio S., Beutler B. (2000): Physical contact between LPS and TLR4 revealed by genetic complementation. Proceedings of the National Academy of Sciences of the United States of America, 97, 2163-2167. Go to original source... Go to PubMed...
    16. Qiu X.T., Li Y.H., Li H.J., Yu Y., Zhang Q. (2007): Localization and tissue expression of swine Toll-like receptor 4 (TLR4). Journal of Agricultural Biotechnology, 15, 37-40.
    17. Shinkai H., Tanaka M., Morozumi T., Eguchi-Ogawa T., Okumura N., Muneta Y., Awata T., Uenishi H. (2006): Biased distribution of single nucleotide polymorphisms (SNPs) in porcine Toll-like receptor 1(TLR1), TLR2, TLR4, TLR5, and TLR6 genes. Immunogenetics, 58, 324-330. Go to original source... Go to PubMed...
    18. Takeuchi O., Hoshino K., Kawai T., Sanjo H., Takada H., Ogawa T., Takeda K., Akira S. (1999): Differential roles of TLR2 and TLR4 in recognition of Gram-negative and Gram-positive bacterial cell wall components. Immunity, 11, 443-451. Go to original source... Go to PubMed...
    19. Vogeli P., Meijerink E., Fries R., Stricker C., Bertschinger H.U. (1997): A molecular test for the detection of E. coli F18 receptors: a breakthrough in the struggle against oedema and post-weaning diarrhoea in swine. Schweizer Archiv für Tierheilkunde, 139, 479-484. Go to PubMed...
    20. Wang X.P., Xu S.Z., Ma T.H., Gao X., Ren H.Y., Chen J. B. (2007): Genetic polymorphism of TLR4 gene and correlation with mastitis in bovine. Acta Veterinaria et Zootechnica Sinica, 38,120-124.
    21. Werling D., Jungi T.W. (2003): Toll-like receptors linking innate and adaptive immune response. Veterinary Immunology and Immunopathology, 91, 1-12. Go to original source... Go to PubMed...
    22. Wu S.L., Yuan Z.W., Ju H.P., Huang X.G., Hua J.D., Shen J.L., Zhou G.Y., Wang J.Y., Xie K.Z., Chen G.H., Zhu G.Q. (2007): Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F 18 fimbrial Escherichia coli in vitro. Chinese Journal of Preventive Veterinary Medicine, 29, 783-787.
    23. Zhou B., Liu C.W., Yu D.B., Huang R.H., Liu H.L., Wang L.Y. (2008): Detection of SNPs in exon 3 of the Swine TLR4 gene using PCR-SSCP method. Animal Husbandry and Veterinary Medicine, 40, 26-30.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content