Czech J. Anim. Sci., 2011, 56(7):305-313 | DOI: 10.17221/1296-CJAS

Effects of selenomethionine and sodium selenite supplementation on meat quality, selenium distribution and antioxidant status in broilers

Y.X. Wang, X.A. Zhan, D. Yuan, X.W. Zhang, R.J. Wu
Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China

This study was conducted to investigate the effects of selenomethionine (Se-Met) and sodium selenite (SS) supplementation on meat quality, selenium (Se) distribution, and antioxidant status in broilers. A total of 360 seven-days-old Ross 308 broilers of an average body weight 162 ± 0.59 g were randomly allotted to three treatments, each of which included three replications of 40 birds. The treatments included a control diet containing 0.04 mg Se/kg and other two diets that contained 0.15 mg Se/kg supplemented by SS or Se-Met. The experiment lasted for 42 days. Selenium supplementation improved (P < 0.05) the Hunter a value of breast muscle in 8 and 16 h and decreased (P < 0.05) the drip loss of breast muscle in 24 and 48 h. Both Se sources and exposed time significantly influenced (P < 0.01) the drip loss of breast muscle. Selenium and glutathione concentrations in serum and in the studied organs were significantly higher (P < 0.05) after dietary Se supplementation while the Se-Met group showed the highest value (P < 0.05). Glutathione peroxidase activity in serum and in the studied organs was also significantly elevated (P < 0.05) by dietary Se supplementation while SS increased the glutathione peroxidase activities in pancreas and breast muscle to a larger extent (P < 0.05) than did Se-Met. The addition of Se from either source caused a significant increase (P < 0.05) in superoxide dismutase activities in tissues (except for kidney) whereas the Se-Met group was more effective (P < 0.05) than the SS group in breast muscle. Selenium supplementation increased (P < 0.05) the total antioxidant capability in serum, liver, kidney and breast muscle while the Se-Met group proved to be more effective (P < 0.05) than the SS group except for kidney. The Se-supplemented diets had a lower (P < 0.05) malondialdehyde concentration in serum and in the studied organs but the effect was more pronounced (P < 0.05) when Se-Met was used except for the serum. These results indicated that Se-Met supplementation was more effective than SS supplementation for depositing Se in serum and tissues, enhancing the antioxidant status and reducing the drip loss of breast muscle.

Keywords: broiler; selenomethionine; drip loss; Hunter a value; antioxidant status

Published: July 31, 2011  Show citation

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Wang YX, Zhan XA, Yuan D, Zhang XW, Wu RJ. Effects of selenomethionine and sodium selenite supplementation on meat quality, selenium distribution and antioxidant status in broilers. Czech J. Anim. Sci. 2011;56(7):305-313. doi: 10.17221/1296-CJAS.
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    References

    1. AOAC (1990): Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Arlington, USA.
    2. Arthur J.R. (2000): The glutathione peroxidases. Cellular and Molecular Life Sciences, 57, 1825-1835. Go to original source... Go to PubMed...
    3. Beistein M.A., Whanger P.D. (1986): Deposition of dietary organic and inorganic Se in rat erythrocyte protein. Journal of Nutrition, 116, 1701-1710. Go to original source... Go to PubMed...
    4. Beutler E., Duron O., Kelly B.M. (1963): Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888. Go to PubMed...
    5. Brigelius-Flohe R. (1999): Tissue-specific functions of individual glutathione peroxidases. Free Radical Biology and Medicine, 27, 951-965. Go to original source... Go to PubMed...
    6. Cantor A.H., Langevin M.L., Noguchi T., Scott M.L. (1975): Efficacy of selenium in selenium compounds and feedstuffs for prevention of pancreatic fibrosis in chicks. Journal of Nutrition, 105, 106-111. Go to original source...
    7. Cantor A.H., Moorehead P.D., Musser M.A. (1982): Comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults. Poultry Science, 61, 478-484. Go to original source... Go to PubMed...
    8. Choct M., Naylor A.J. (2004): The effect of dietary selenium source and vitamin E levels on performance of male broilers. Asian-Australasian Journal of Animal Science, 17, 1000-1006. Go to original source...
    9. Choct M., Naylor A.J., Reinke N. (2004): Selenium supplementation affects broiler growth performance, meat yield and feather coverage. British Poultry Science, 45, 677-683. Go to original source... Go to PubMed...
    10. Forstrom J.W., Zakowski J.J., Tappel A.L. (1978): Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry, 17, 2639-2644. Go to original source... Go to PubMed...
    11. Ga'miz-Gracia L., Luque de Castro M.D. (1999): Determination of selenium in nutritional supplements and shampoos by flow injection-hydride generation-atomic fluorescence spectrometry. Talanta, 50, 875-880. Go to original source... Go to PubMed...
    12. Henry P.R., Ammerman C.B. (1995): Selenium bioavailability. In: Ammerman C.B., Baker D.H., Lewis A.J. (eds): Bioavailability of Nutrients for Animals. Academic Press, New York, USA, 301 pp. Go to original source...
    13. James B.W., Goodband R.D., Unruh J.A., Tokach M.D., Nelssen J.L., Dritz S.S., O'Quinn P.R., Andrews B.S. (2002): Effect of creatine monohydrate on finishing pig growth performance, carcass characteristics and meat quality. Animal Feed Science and Technology, 96, 135-145. Go to original source...
    14. Kim Y.Y., Mahan D.C. (2001): Comparative effects of high dietary levels of organic and inorganic Se on Se toxicity of growing finishing pigs. Journal of Animal Science, 79, 942-948. Go to original source... Go to PubMed...
    15. Kohrle J., Brigelius-Flohe R., Bock A., Gartner R., Meyer O., Flohe L. (2000): Se in biology: facts and medical perspectives. The Journal of Biological Chemistry, 381, 849-864. Go to original source... Go to PubMed...
    16. Kuricová S., Boldižárová K., Grešáková L., Bobcek R., Levkut M., Lenk L. (2003): Chicken selenium status when fed a diet supplemented with Se-yeast. Acta Veterinaria, 72, 339-346. Go to original source...
    17. Lawrence R.A., Burk R.F. (1976): Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications, 71, 952-958. Go to original source... Go to PubMed...
    18. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (1951): Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193, 265-275. Go to original source... Go to PubMed...
    19. Mahan D.C., Cline T.R., Richert B. (1999): Effects of dietary levels of selenium enriched yeast and sodium selenite as selenium sources fed to growing-finishing pigs on performance, tissue selenium, serum glutathione peroxidase activity, carcass characteristics and loin quality. Journal of Animal Science, 77, 2172-2179. Go to original source... Go to PubMed...
    20. Mahan D.C., Parrett N.A. (1996): Evaluating the efficacy of selenium-enriched yeast and sodium selenite on tissue selenium retention and serum glutathione peroxidase activity in grower and finisher swine. Journal of Animal Science, 74, 2967-2974. Go to original source... Go to PubMed...
    21. Mahmoud K.Z., Edens F.W. (2003): Influence of selenium sources on age related and mild heat stress-related changes of blood and liver glutathione redox cycle in broiler chickens (Gallus domesticus). Comparative Biochemistry and Physiology-Part B: Biochemistry & Molecular Biology, 136, 921-934. Go to original source... Go to PubMed...
    22. Mateo R.D., Spallholz J.E., Elder R., Yoon I., Kim S.W. (2007): Efficacy of dietary selenium sources on growth and carcass characteristics of growing-finishing pigs fed diets containing high endogenous selenium. Journal of Animal Science, 85, 1177-1183. Go to original source... Go to PubMed...
    23. Moksnes K., Norheim G. (1986): A comparison of selenomethionine and sodium selenite as a supplement in chicken feeds. Acta Veterinaria Scandinavica, 27, 103-114. Go to original source... Go to PubMed...
    24. Mruk D.D., Silvestrini B., Mo M.Y., Cheng C.Y. (2002): Antioxidant superoxide dismutase - a review: its function, regulation in the testis, and role in male fertility. Contraception, 67, 305-311. Go to original source... Go to PubMed...
    25. NRC (1994): Nutrient Requirements of Poultry. 9th rev. ed. National Academy Press, Washington, USA.
    26. Panckenko L.F., Brusov O.S., Gerasimov A.M., Loktaeva A.E. (1975): Intramitochondrial localization and release of rat liver superoxide dismutase. FEBS Letters, 55, 84-87. Go to original source... Go to PubMed...
    27. Payne R.L., Southern L.L. (2005a): Comparison of inorganic and organic selenium sources for broilers. Poultry Science, 84, 898-902. Go to original source... Go to PubMed...
    28. Payne R.L., Southern L.L. (2005b): Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium. Poultry Science, 84, 1268-1276. Go to original source... Go to PubMed...
    29. Pi Z.K., Wu Y.M., Liu J.X. (2005): Effect of pretreatment and pelletization on nutritive value of rice straw-based total mixed ration, and growth performance and meat quality of growing Boer goats fed on TMR. Small Ruminant Research, 56, 81-88. Go to original source...
    30. Placer Z.A., Cushman L.L., Johnson B.C. (1966): Estimation of product of lipid peroxidation (malondialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359-364. Go to original source... Go to PubMed...
    31. Rayman M.P. (2000): The importance of selenium to human health. Lancet, 365, 233-241. Go to original source... Go to PubMed...
    32. Schaefer D.M., Liu Q.P., Faustman C., Yin M.C. (1995): Supranutritional administration of vitamins E and C improves oxidative stability of beef. Journal of Nutrition, 125, 1792-1798.
    33. Schrauzer G.N. (2000): Selenomethionine: a review of its nutritional significance, metabolism, and toxicity. Journal of Nutrition, 130, 1653-1656. Go to original source... Go to PubMed...
    34. Schrauzer G.N. (2003): The nutritional significance, metabolism and toxicology of selenomethionine. Advances in Food and Nutrition Research, 47, 73-112. Go to original source... Go to PubMed...
    35. Skřivan M., Dlouhá G., Mašata O., Ševčíková S. (2008): Effect of dietary selenium on lipid oxidation, selenium and vitamin E content in the meat of broiler chickens. Czech Journal of Animal Science, 53, 306-311. Go to original source...
    36. Skřivan M., Bubancová I., Marounek M., Dlouhá G. (2010): Selenium and α-tocopherol content in eggs produced by hens that were fed diets supplemented with selenomethionine, sodium selenite and vitamin E. Czech Journal of Animal Science, 55, 388-397. Go to original source...
    37. Sunde R.A., Hoekstra W.G. (1980): Incorporation of selenium from selenite into selenocysteine into glutathione peroxidase in the isoloated perfused rat liver. Biochemical and Biophysical Research Communications, 93, 1181-1188. Go to original source... Go to PubMed...
    38. Surai P.F. (2002): Selenium in poultry nutrition. 2. Reproduction, egg and meat quality and practical applications. World's Poultry Science Journal, 58, 431-450. Go to original source...
    39. Surai P.F., Dvorska J.E. (2002): Effect of selenium and vitamin E content of the diet on lipid peroxidation in breast muscle tissue of broiler breeder hens during storage. Australian Poultry Science Symposium, 14, 187-192.
    40. White C.L., Hoekstra W.G. (1979): The metabolism of selenite and selenomethionine in mouse fibroblasts grown in tissues culture. Biological Trace Element Research, 1, 243-257. Go to original source... Go to PubMed...
    41. Wolfram S., Berger B., Grenacher B., Scharrer E. (1989): Transport of seleno amino acids and their sulphur analogues across the intestinal brush border membrane. Journal of Nutrition, 119, 706-712. Go to original source... Go to PubMed...
    42. Zhan X.A., Wang M., Zhao R.Q., Li W.F., Xu Z.R. (2007): Effects of different selenium source on selenium distribution, loin quality and antioxidant status in finishing pigs. Animal Feed Science and Technology, 132, 202-211. Go to original source...

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