Czech J. Anim. Sci., 2007, 52(11):399-406 | DOI: 10.17221/2322-CJAS

Fatty acid profiles of rumen fluid from sheep fed diets supplemented with various oils and effect on the rumen ciliate population

Z. Váradyová, S. Ki¹idayová, P. Siroka, D. Jalè
Institute of Animal Physiology, Slovak Academy of Sciences, Ko¹ice, Slovak Republic

The profile of trans-fatty acids and the protozoan population were evaluated in four ruminally fistulated sheep fed a diet with meadow hay: barley grain ratio (80:20%) plus sunflower oil (SO), rapeseed oil (RO) or linseed oil (LO) (5% wt/wt). The concentrate was daily mixed with individual oils and offered at 07.00 h. A 4 × 4 Latin square with 4-week periods was used. The concentration of trans-vaccenic acid (TVA) was the highest 4 h after feeding (36.1 g/100 g FA with SO; 34.5 g/100 g FA with RO) and then decreased with the time after feeding (P < 0.05). The concentration of cis9, trans11 conjugated linoleic acid (c9,t11-CLA) with RO increased from 3.23 g/100 g FA (2 h after feeding) to 4.67 g/100 g FA (4 h after feeding). The concentration of c9,t11-CLA with SO increased from 2.09 g/100 g FA (2 h after feeding) to 2.31 g/100 g FA (4 h after feeding). The concentration of c9,t11-CLA with LO was the highest 4 h after feeding (2.07 g/100 g FA). Overall effects of the oil supplements and time after feeding on short-chain fatty acids (SCFA), medium-chain fatty acids (MCFA) and long-chain fatty acids (LCFA) in the rumen fluid were evident. A strong interaction of oil supplements and time after feeding was detected in the concentration of UFA and SFA (P < 0.001). A significant effect of LO on the rumen ciliate population was observed; the total protozoan concentration and the number of Entodinium spp. were decreased as well as Dasytricha ruminantium, Isotricha spp., Polyplastron multivesiculatum, Ophryoscolex c. tricoronatus and Eremoplastron dilobum.

Keywords: sunflower oil; rapeseed oil; linseed oil; fatty acids; rumen ciliates

Published: November 30, 2007  Show citation

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Váradyová Z, Ki¹idayová S, Siroka P, Jalè D. Fatty acid profiles of rumen fluid from sheep fed diets supplemented with various oils and effect on the rumen ciliate population. Czech J. Anim. Sci. 2007;52(11):399-406. doi: 10.17221/2322-CJAS.
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