Czech Journal of Animal Science, 2020 (vol. 65), issue 2
The effect of milking frequency on milk yield and milk composition in ewesReview
Lucia Mačuhová, Vladimír Tančin, Juliana Mačuhová
Czech J. Anim. Sci., 2020, 65(2):41-50 
Milking frequency is the main factor regulating milk yield and milk quality if feeding, welfare, health, and environmental conditions are adequate. Milk yield and composition are substantially influenced by breed, and consequently the breed has an impact on both factors at a different frequency of milking. Further, the size of cisternal compartment plays an important role in accommodating secreted milk between milkings. Thus, ewes with large cisterns (i.e. large cisternal area, high cisternal milk percentage, and delayed tight junction opening during milk stasis) adapt themselves better to longer milking intervals than ewes with a small cistern. The...
Effects of different dietary lipid sources on fatty acid composition and gene expression in common carpOriginal Paper
Hong-Tao Ren, Guang-Qin Zhang, Yong Huang, Xiao-Chan Gao
Czech J. Anim. Sci., 2020, 65(2):51-57 | DOI: 10.17221/248/2019-CJAS
The effects of fatty acid composition in artificial feed on the change in the fatty acid composition of carp muscles and the relationship between Δ6-Fad and Elovl5 genes participating in the regulation of fatty acid synthesis were studied. Juveniles were fed three semi-purified diets (D1-D3) for 6 weeks with different lipid sources: D1, fish oil with high highly unsaturated fatty acids (HUFA); D2, corn oil with high linoleic acid (18:2n-6, LA), D3, linseed oil with high α-linolenic acid (18:3n-3, LNA); then, samples were taken to explore the molecular mechanism and the factors which affect the synthesis of carp HUFA. The...
The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horsesOriginal Paper
Renata Kolláthová, Branislav Gálik, Marko Halo, Anton Kováčik, Ondrej Hanušovský, Daniel Bíro, Michal Rolinec, Miroslav Juráček, Milan Šimko
Czech J. Anim. Sci., 2020, 65(2):58-65 | DOI: 10.17221/181/2019-CJAS
Twelve adult Slovak warmblood sport horses were used to study the effect of dried grape pomace (DGP) on health through blood serum biochemical indicators, and on apparent total tract digestibility of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF). The digestibility analysis was carried out by two in vivo methods, total faeces collection (TFC) and using lignin as a marker (ADL). Animals were divided into 3 groups: control group (C, without supplementation), experimental group 1 (E1, feed rations + 200 g of DGP) and experimental group 2 (E2, feed rations + 400 g of DGP)....
Comparing the stemness and morphobiometry of spermatogonial stem cells from Doom pig on different days of cultureOriginal Paper
Arpana Das, Dipak Bhuyan, Partha Pratim Das, Simanta Koushik, Bula Das, Arundhati Phookan, Suresh Dinkar Kharche, Shiva Pratap Singh, Manmohan Singh Chauhan
Czech J. Anim. Sci., 2020, 65(2):66-76
The present study was conducted to compare the stemness and morphobiometry of spermatogonial stem cells (SSCs) from the Doom pig on different days of culture (9th, 30th and 65th day) for the development of long-term culture method. The testes from 7-15-day old piglets were collected and two-step enzymatic digestion was used to isolate SSCs. Before in vitro culture of SSC-like cells on the Sertoli cell feeder layer, the cells were enriched by differential plating and Percoll density gradient centrifugation. The isolated SSCs were characterised by alkaline phosphatase and immunofluorescence staining and qPCR...