Czech J. Anim. Sci., 2020, 65(2):58-65 | DOI: 10.17221/181/2019-CJAS
The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horsesOriginal Paper
- 1 Department of Animal Nutrition, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovakia
- 2 Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovakia
- 3 Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Twelve adult Slovak warmblood sport horses were used to study the effect of dried grape pomace (DGP) on health through blood serum biochemical indicators, and on apparent total tract digestibility of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF). The digestibility analysis was carried out by two in vivo methods, total faeces collection (TFC) and using lignin as a marker (ADL). Animals were divided into 3 groups: control group (C, without supplementation), experimental group 1 (E1, feed rations + 200 g of DGP) and experimental group 2 (E2, feed rations + 400 g of DGP). In animals, no health problems were detected during the trial. Of the blood serum indicators, only the concentrations of potassium (increase in E2 group compared to C group) and alanine aminotransferase (decrease in E2 group in comparison with E1 and C group) were affected (P < 0.05). The ADL method resulted in underestimated digestibility coefficients due to low recovery rates of lignin (less than 90%) in C group and E1 group. According to TFC, in E1 group higher digestibility coefficients were detected for DM, OM and CP (P > 0.05) compared to C group. However, in E2 group lower digestibility of all the studied nutrients was found (P > 0.05) in comparison with C group and E1 group. These results suggest that DGP could be used in horse diets up to 200 g without negative effect on their health and for a possible digestibility improvement of some nutrients.
Keywords: equine; metabolic tests; nutrition; utilization; wine by-products
Published: February 29, 2020 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Aditya S, Ohh SJ, Ahammed M, Lohakare J. Supplementation of grape pomace (Vitis vinifera) in broiler diets and its effect on growth performance, apparent total tract digestibility of nutrients, blood profile and meat quality. Anim Nutr. 2018;4:210-214.
Go to original source...
Go to PubMed...
- AOAC. Official Methods of Analysis. Gaithersburg, USA: Association of Official Analytical Chemists International; 2000.
- Araujo KV, Lima JAF, Fialho ET, Miyagi ES. Comparison among the internal markers and the total collection method in the determination of the forage nutrients digestibilities in equine. R. Bras. Zootec. 2000;29:745-751. (Portuguese)
Go to original source...
- Arbabi S, Ghoorchi T, Parvar R. A comparison of apparent digestibility of nutrient in Caspian horse feeds as determined by total collection of faeces, acid insoluble ash and lignin methods. Iran. J. Appl. Anim. Sci. 2016;6:461-466.
- Bahrami Y, Foroozandeh AD, Zamani F, Modarresi M, Eghbal-Saeid S, Chekani-Azar S. Effect of diet with varying levels of dried grape pomace on dry matter digestibility and growth performance of male lambs. J. Anim. Plant Sci. 2010;6:605-610.
- Baumgartel T, Kluth H, Epperlein K, Rodehutscord M. A note on digestibility and energy value for sheep of different grape pomace. Small Rumin. Res. 2007;67:302-306.
Go to original source...
- Bekhit AEA, Cheng VJ, Harrison R, Ye ZJ, Bekhit AA, Ng TB, Kong LM. Technological aspects of by-product utilization. In: Bordiga M, editor. Valorization of Wine Making ByProducts. Boca Raton, USA: CRC Press; 2016. pp. 117-198.
Go to original source...
- Bergero D, Meineri G, Miraglia N, Peiretti PG. Apparent digestibility of hays in horses determined by total collection of faeces and using internal marker methods. J. Food Agric. Environ. 2005;3:199-202.
- Bergero D, Prefontaine C, Miraglia N, Peiretti PG. A comparison between the 2 N and 4 N HCl acid-insoluble ash methods for digestibility trials in horses. Animal. 2009;3:1728-1732.
Go to original source...
Go to PubMed...
- Brenes A, Viveros A, Chamorro S, Arija I. Use of polyphenolrich grape by-products in monogastric nutrition. A review. Anim. Feed Sci. Technol. 2016;211:1-17.
Go to original source...
- Chachulowa J, Lojek J, Chrzanowski S, Chachula J, Stroziewski J. A comparison of digestibility coefficients of nutrients in horse feeds as determined by conventional and lignin methods. J. Anim. Feed Sci. 1994;3:239-246.
Go to original source...
- Chamorro S, Viveros A, Rebole A, Rica A, Arija BD, Brenes AA. Influence of dietary enzyme addition on polyphenol utilization and meat lipid oxidation of chicks fed grape pomace. Food Res. Int. 2015;73:197-203.
Go to original source...
- Chedea VS, Palade LM, Marin DE, Pelmus RS, Habeanu M, Rotar MC, Gras MA, Pistol GC, Taranu I. Intestinal absorption and antioxidant activity of grape pomace polyphenols. Nutrients. 2018;10:588-612.
Go to original source...
Go to PubMed...
- Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance). OJEU 2009;L54:1.
- Danko J, Simon F, Artimova J. (editors). Nomina Anatomica Veterinaria. Košice, Slovakia: The University of Veterinary Medicine and Pharmacy; 2011. (Slovak)
- Davies JA, Krebs GL, Barnes A, Pant I, McGrath PJ. Feeding grape seed extract to horses: Effects on health, intake and digestion. Animal 2008;3:380-384.
Go to original source...
Go to PubMed...
- Dominguez J, Martinez-Cordeiro H, Lores M. Earthworms and grape marc: Simultaneous production of a high-quality biofertilizer and bioactive-rich seeds. In: Morata A, Loira I, editors. Grape and Wine Biotechnology. Rijeka, Croatia: IntechOpen; 2016. pp. 167-183.
Go to original source...
- Doubek J, Slosarkova S, Rehakova K, Bouda J, Scheer P, Piperisova I, Tomenendalova J, Matalova E, editors. The Interpretation of Basic Biochemical and Hematological Findings in Animals. Brno, Czech Republic: Noviko; 2010. (Czech)
- Foiklang S, Wanapat M, Norrapoke T. In vitro rumen fermentation and digestibility of buffaloes as influenced by grape pomace powder and urea treated rice straw supplementation. Anim. Sci. J. 2015;87:370-377.
Go to original source...
Go to PubMed...
- Foiklang S, Wanapat M, Norrapoke T. Effect of grape pomace powder, mangosteen peel powder and monensin on nutrient digestibility, rumen fermentation, nitrogen balance and microbial protein synthesis in dairy steers. AsianAustralas. J. Anim. Sci. 2016;29:1416-1423.
Go to original source...
Go to PubMed...
- Galik B, Biro D, Simko M, Juracek M, Rolinec M, Hanusovsky O. Feeds in ruminants and non-ruminants nutrition. Nitra, Slovakia: Slovak Agriculture University in Nitra; 2018. (Slovak)
- Galik B, Kollathova R, Rolinec M, Juracek M, Simko M, Hanusovsky O, Biro D, Vasekova P, Kolesarova A, Barantal S. Grape by-products as bioactive substances in animal nutrition: A review. Agr. Food. 2019;7:167-172.
- Goachet AG, Philippeau C, Varloud M, Julliand V. Adaptations to standard approaches for measuring total tract apparent digestibility and gastro-intestinal retention time in horses in training. Anim. Feed Sci. Technol. 2009;152:141-151.
Go to original source...
- Ishida K, Kishi Y, Oishi K, Hirooka H, Kumagai H. Effects of feeding polyphenol-rich winery wastes on digestibility, nitrogen utilization, ruminal fermentation, antioxidant status and oxidative stress in wethers. Anim. Sci. J. 2015;86:260-269.
Go to original source...
Go to PubMed...
- Kerasioti E, Terzopoulou Z, Komini O, Kafantaris I, Makri S, Stagos D, Gerasopoulos K, Anisimov NY, Tsatsakis AM, Kouretas D. Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep. Toxicol. Rep. 2017;4:364-372.
Go to original source...
Go to PubMed...
- Kolesarova A, Slamecka J, Jurcik R, Tataruch F, Lukac N, Kovacik J, Capcarova M, Valent M, Massanyi P. Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters. J. Environ. Sci. Health A Tox. Hazard. Subst. Environ. Eng. 2008;43:646-650.
Go to original source...
Go to PubMed...
- Kovacik A, Arvay J, Tusimova E, Harangozo L, Tvrda E, Zbynovska K, Cupka P, Andrascikova S, Tomas J, Massanyi P. Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters. Chemosphere 2017;168:365-371.
Go to original source...
Go to PubMed...
- Lichovnikova M, Kalhotka L, Adam V, Klejdus B, Anderle V. The effects of red grape pomace inclusion in grower diet on amino acid digestibility, intestinal microflora, and sera and liver antioxidant activity in broilers. Turk. J. Vet. Anim. Sci. 2015;39:406-412.
Go to original source...
- Makkar HPS. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effect of feeding tannin-rich feeds. Small Rumin. Res. 2003;49:241-256.
Go to original source...
- Makris DP, Boskou G, Andrikopoulos NK, Kefalas P. Characterisation of certain major polyphenolic antioxidants in grape (Vitis vinifera cv. Roditis) stems by liquid chromatography-mass spectrometry. Eur. Food Res. Technol. 2007;226:1075-1079.
Go to original source...
- Miraglia N, Bergero D, Bassano B, Tarantol M, Ladett G. Studies of apparent digestibility in horses and the use of internal markers. Livest. Prod. Sci. 1999;60:21-25.
Go to original source...
- Nistor E, Dobrei A, Dobrei A, Bampidis V, Ciolac V. Grape pomace in sheep and dairy cows feeding. JHFB 2014;18:146-150.
- NRC. Nutrient Requirements of Horses. 6th Revised Edn. Washington, DC, USA: National Academies Press; 2007.
- Oliveira CAA, Almeida FQ,Valaderes Filho SC, Vieira AA, Almeida MIV, Corassa AL, Lopes BA, Macedo R. Estimate of apparent digestibility of nutrients in horses diets, using chromic oxide and internal markers. Rev. Bras. Zootecn. 2003;32:1681-1689. (Portuguese).
Go to original source...
- Oliveira K, Costa C, Bittar CMM, Santos VP, Oliveira VAB, Sa JC. Indigestible cellulose and lignin in determining faeces production and apparent digestibility in horses. Acta Sci. 2012;34:267-272.
Go to original source...
- Ozduven M, Coscuntuna L, Koc F. Determination of fermantation and feed value characteristics of grape pomace silage. Trak. Univ. J. Nat. Sci. 2005;6:45-50.
- Peiretti PG, Miraglia N, Meineri G, Polidori M, Bergero D. Apparent digestibility assessment of concentrate feed in horses: A comparison among some different methods. In: Miraglia N, Bergero D, editors. Proc. 5th Conference on New Findings in Equine Practices, Turin, Italy; 2003. pp. 49-57.
- Prochniak T, Rozempolska-Rucinska I, Zieba G. Maternal effect on sports performance traits in horses. Czech J. Anim. Sci. 2019;64:361-365.
Go to original source...
- Rondeau P, Gambier F, Jolibert F, Brosse N. Compositions and chemical variability of grape pomaces from French vineyard. Ind. Crops Prod. 2013;43:251-254.
Go to original source...
- Sales J. A review on the use of indigestible dietary markers to determine total tract apparent digestibility of nutrients in horses. Anim. Feed Sci. Technol. 2012;174:119-130.
Go to original source...
- Siqueira RF, Gomes FC, Rodrigues PHM, Fukushima RS, Lorenzo CLF, Gobesso AAO. Use of cutin for determining apparent digestibility of diets for horses. Arq Bras Med Vet Zootec. 2009;61:1373-1381.
Go to original source...
- Somogyvari E, Posta J, Mihok S. Genetic analysis of the Hungarian population of endangered Hucul horse. Czech J. Anim. Sci. 2018;63:237-246.
Go to original source...
- Strakova E, Suchy P. Content of nutrients and energy in pasture vegetation and their apparent digestibility in Old Kladruber horses. Acta Vet. Brno. 2013;82:37-41.
Go to original source...
- Teixeira A, Baenas N, Dominguez-Perles R, Barros A, Rosa E, Moreno DA, Garcia-Viguera C. Natural bioactive compounds from winery by-products as health promoters: A review. Int. J. Mol. Sci. 2014;15:15638-15678.
Go to original source...
Go to PubMed...
- Vinyard J, Chibisa G. Nutrient intake, ruminal and apparent total tract digestibility, and nitrogen utilization in finishing cattle fed increasing amounts of grape pomace. J. Anim. Sci. 2019;96:436.
Go to original source...
- Viveros A, Chamorro S, Pizarro M, Arija I, Centeno C, Brenes A. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult. Sci. 2011;90:566-578.
Go to original source...
Go to PubMed...
- Zalikarenab L, Pirmohammadi R, Teimuriyalsari A. Chemical composition and digestibility of white and red grape pomace for ruminants. J. Anim. Vet. Adv. 2007;6:1107-1111.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.