Czech Journal of Animal Science, 2017 (vol. 62), issue 4

Do rearing system and free-range stocking density affect meat quality of chickens fed feed mixture with rapeseed oil?Original Paper

Věra Skřivanová, Eva Tůmová, Michaela Englmaierová, Darina Chodová, Miloš Skřivan

Czech J. Anim. Sci., 2017, 62(4):141-149 | DOI: 10.17221/79/2016-CJAS  

Recently, consumers have paid an attention to animal-friendly meat. The aim of the study was to evaluate the meat quality of breast muscles in indoor and free-range chickens with respect to the stocking density of outdoor chickens (8.3 or 4.15 birds per m2) fed feed with rapeseed oil. The free-range chickens reached 96 and 97% of the final live weight of the indoor chickens and their feed : gain ratio was by 7 and 9% higher. The pasture intake had a small effect on the breast meat quality and was presumably associated with rapeseed oil in the basal diet which strongly influenced meat monounsaturated and polyunsaturated fatty acid content...

Up-regulation of oxytocin receptor gene and protein in the sheep anterior pituitary by a dopamine derivative (salsolinol)Original Paper

Konrad Górski, Małgorzata Hasiec, Marlena Zielińska-Górska, Ferenc Fülöp, Tomasz Misztal

Czech J. Anim. Sci., 2017, 62(4):150-156 | DOI: 10.17221/30/2016-CJAS  

Specific oxytocin receptors (OTR) have been identified in the anterior pituitary (AP), and their expression has been shown to change in relation to the animal physiological stage, whereas salsolinol (a derivative of dopamine) has been shown to stimulate the synthesis and release of oxytocin (OT) in lactating sheep. In the present study, the expression of both OTR mRNA and OTR protein in the AP were examined by real-time quantitative PCR and enzyme-linked immunosorbent assay, either in anestrous or lactating sheep 48 h after weaning lambs. Moreover, the effect of salsolinol administered via an intracerebroventricular (i.c.v.) infusion was tested...

Genetic variations of the bovine MX1 and their association with mastitisOriginal Paper

Ningbo Chen, Fengqiao Wang, Nongqi Yu, Yuan Gao, Jieping Huang, Yongzhen Huang, Xianyon Lan, Chuzhao Lei, Hong Chen, Ruihua Dang

Czech J. Anim. Sci., 2017, 62(4):157-167 | DOI: 10.17221/97/2015-CJAS  

The primary agent of mastitis is a wide spectrum of bacterial strains; however, viral-related mastitis has also been reported. The MX dynamin-like GTPase 1 (MX1) gene has been demonstrated to confer positive antiviral responses to many viruses, and may be a suitable candidate gene for the study of disease resistance in dairy cattle. The present study was conducted to investigate the genetic diversity of the MX1 gene in Chinese cattle breeds and its effects on mastitis in Holstein cows. First, polymorphisms were identified in the complete coding region of the bovine MX1 gene in 14 Chinese cattle breeds. An association...

Beneficial effect of bacteriocin-producing strain Enterococcus durans ED 26E/7 in model experiment using broiler rabbitsOriginal Paper

Andrea Lauková, Monika Pogány Simonová, Ľubica Chrastinová, Anna Kandričáková, Jana Ščerbová, Iveta Plachá, Klaudia Čobanová, Zuzana Formelová, Ľubomír Ondruška, Gabriela Štrkolcová, Viola Strompfová

Czech J. Anim. Sci., 2017, 62(4):168-177 | DOI: 10.17221/21/2016-CJAS  

From the aspect of probiotic properties and bacteriocins, Enterococcus faecium belongs to the most frequently studied species among enterococci. This study deals with testing the strain of the species Enterococcus durans ED 26E/7 in broiler rabbits. The strain ED 26E/7 isolated from ewes lump cheese produces an antimicrobial substance durancin. Forty-eight post-weaned rabbits (aged 5 weeks) of both sexes were divided into experimental group (EG) and control group (CG) per 24 animals each, and kept in standard cages, two animals per cage. EG group rabbits were additionally administered the ED 26E/7 strain (500 µl/animal/day)...

Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigsOriginal Paper

Wenjing Jiao, Qingquan Ma, Xiting Lv, Anshan Shan, Zhongyu Li

Czech J. Anim. Sci., 2017, 62(4):178-183 | DOI: 10.17221/84/2015-CJAS  

β-Defensins are a major group of mammalian antimicrobial peptides and play an important role in innate and adaptive immunity due to their antimicrobial, chemotactic, and regulatory activities. In this study, the tissue distribution of porcine β-defensin (pBD-1, pBD-2, and pBD-3) gene expression was determined using real-time polymerase chain reaction in 14-day-old Chinese Min pigs and Landrace pigs. The results showed that in the two breeds, pBD-1 and pBD-2 were primarily expressed in the tongue and kidney, respectively. The pBD-3 genes in Min pigs and Landrace pigs were abundant in the...