Czech J. Anim. Sci., 2017, 62(4):178-183 | DOI: 10.17221/84/2015-CJAS

Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigsOriginal Paper

Wenjing Jiao, Qingquan Ma, Xiting Lv, Anshan Shan*, Zhongyu Li
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, P.R. China

β-Defensins are a major group of mammalian antimicrobial peptides and play an important role in innate and adaptive immunity due to their antimicrobial, chemotactic, and regulatory activities. In this study, the tissue distribution of porcine β-defensin (pBD-1, pBD-2, and pBD-3) gene expression was determined using real-time polymerase chain reaction in 14-day-old Chinese Min pigs and Landrace pigs. The results showed that in the two breeds, pBD-1 and pBD-2 were primarily expressed in the tongue and kidney, respectively. The pBD-3 genes in Min pigs and Landrace pigs were abundant in the heart and tongue respectively. The mRNA expression levels of the three peptide genes in most tissues were much higher in Min pigs than in Landrace pigs. Collectively, higher defensin gene expressions were observed in some organs and tissues of Min pigs, presumably related to their higher resistance to disease.

Keywords: antimicrobial peptides; swine; mRNA abundance

Published: April 30, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Jiao W, Ma Q, Lv X, Shan A, Li Z. Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigs. Czech J. Anim. Sci. 2017;62(4):178-183. doi: 10.17221/84/2015-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Auvynet C., Rosenstein Y. (2009): Multifunctional host defense peptides: antimicrobial peptides, the small yet big players in innate and adaptive immunity. FEBS Journal, 276, 6497-6508. Go to original source... Go to PubMed...
    2. Bagnicka E., Strzalkowska N., Jozwik A., Krzyzewski J., Horbanczuk J., Zwierzchowski L. (2010): Expression and polymorphism of defensins in farm animals. Acta Biochimica Polonica, 57, 487-497. Go to original source... Go to PubMed...
    3. Bergman P., Johansson L., Wan H., Jones A., Gallo R.L., Gudmundsson G.H., Hokfelt T., Jonsson A.B., Agerberth B. (2006): Induction of the antimicrobial peptide CRAMP in the blood-brain barrier and meninges after meningococcal infection. Infection and Immunity, 74, 6982-6991. Go to original source... Go to PubMed...
    4. Cederlund A., Gudmundsson G.H., Agerberth B. (2011): Antimicrobial peptides important in innate immunity. FEBS Journal, 278, 3942-3951. Go to original source... Go to PubMed...
    5. Chen J., Qi S., Guo R., Yu B., Chen D. (2010): Different messenger RNA expression for the antimicrobial peptides beta-defensins between Meishan and crossbred pigs. Molecular Biology Reports, 37, 1633-1639. Go to original source... Go to PubMed...
    6. Dommisch H., Jepsen S. (2015): Diverse functions of defensins and other antimicrobial peptides in periodontal tissues. Periodontology 2000, 69, 96-110. Go to original source... Go to PubMed...
    7. Elahi S., Buchanan R.M., Attah-Poku S., Townsend H.G.G., Babiuk L.A., Gerdts V. (2006): The host defense peptide beta-defensin 1 confers protection against Bordetella pertussis in newborn piglets. Infection and Immunity, 74, 2338-2352. Go to original source... Go to PubMed...
    8. Hancock R.E.W., Sahl H.-G. (2006): Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nature Biotechnology, 24, 1551-1557. Go to original source... Go to PubMed...
    9. Hazlett L., Wu M.H. (2011): Defensins in innate immunity. Cell and Tissue Research, 343, 175-188. Go to original source... Go to PubMed...
    10. Hiratsuka T., Nakazato M., Date Y., Mukae H., Matsukura S. (2001). Nucleotide sequence and expression of rat betadefensin-1: its significance in diabetic rodent models. Nephron, 88, 65-70. Go to original source... Go to PubMed...
    11. Huttner K.M., Kozak C.A., Bevins C.L. (1997): The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Letters, 413, 45-49. Go to original source... Go to PubMed...
    12. Huttner K.M., Brezinski-Caliguri D.J., Mahoney M.M., Diamond G. (1998): Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract. The Journal of Nutrition, 128, 297-299. Go to original source... Go to PubMed...
    13. Ma Q.Q., Jiao W.J., Wang Z.Y., Wu C.X., Shan A.S., Wang Y.B., Cai J.B. (2014): Tissue specificity and species superiority of cathelicidin gene expression in Chinese indigenous Min pigs. Livestock Science, 161, 36-40. Go to original source...
    14. McCormick T.S., Weinberg A. (2010): Epithelial cell-derived antimicrobial peptides are multifunctional agents that bridge innate and adaptive immunity. Periodontology 2000, 54, 195-206. Go to original source... Go to PubMed...
    15. Oppenheim J.J., Biragyn A., Kwak L.W., Yang D. (2003): Roles of antimicrobial peptides such as defensins in innate and adaptive immunity. Annals of the Rheumatic Diseases, 62, 17-21. Go to original source... Go to PubMed...
    16. Qi S., Chen J., Guo R., Yu B., Chen D. (2009): Beta-defensins gene expression in tissues of the crossbred and Tibetan pigs. Livestock Science, 123, 161-168. Go to original source...
    17. Sang Y.M., Patil A.A., Zhang G.L., Ross C.R., Blecha F. (2006): Bioinformatic and expression analysis of novel porcine beta-defensins. Mammalian Genome, 17, 332-339. Go to original source... Go to PubMed...
    18. Sang Y., Blecha F. (2009): Porcine host defense peptides: expanding repertoire and functions. Developmental and Comparative Immunology, 33, 334-343. Go to original source... Go to PubMed...
    19. Scott M.G., Hancock R.E. (2000): Cationic antimicrobial peptides and their multifunctional role in the immune system. Critical Reviews in Immunology, 20, 407-431. Go to original source... Go to PubMed...
    20. Selsted M.E., Ouellette A.J. (2005): Mammalian defensins in the antimicrobial immune response. Nature Immunology, 6, 551-557. Go to original source... Go to PubMed...
    21. Soman S.S., Nair S., Issac A., Arathy D.S., Niyas K.P., Anoop M., Sreekumar E. (2009): Immunomodulation by duck defensin, Apl_AvBD2: in vitro dendritic cell immunoreceptor (DCIR) mRNA suppression, and B- and T-lymphocyte chemotaxis. Molecular Immunology, 46, 3070-3075. Go to original source... Go to PubMed...
    22. Stolzenberg E.D., Anderson G.M., Ackermann M.R., Whitlock R.H., Zasloff M. (1997): Epithelial antibiotic induced in states of disease. Proceedings of the National Academy of Sciences of the United States of America, 94, 8686-8690. Go to original source... Go to PubMed...
    23. Veldhuizen E.J.A., van Dijk A., Tersteeg M.H.G., Kalkhove S.I.C., van der Meulen J., Niewold T.A., Haagsman H.P. (2007): Expression of beta-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: lack of upregulation in vivo upon Salmonella typhimurium infection. Molecular Immunology, 44, 276-283. Go to original source... Go to PubMed...
    24. Wang W., Cole A.M., Hong T., Waring A.J., Lehrer R.I. (2003): Retrocyclin, an antiretroviral theta-defensin, is a lectin. Journal of Immunology, 170, 4708-4716. Go to original source... Go to PubMed...
    25. Xu Z.Y. (1989): Breed Characteristics of Indigenous Pig Breeds in China. Zhejiang Scientific and Technical Press, Hangzhou, China.
    26. Yang D., Chertov O., Bykovskaia S.N., Chen Q., Buffo M.J., Shogan J., Anderson M., Schroder J.M., Wang J.M., Howard O.M., Oppenheim J.J. (1999): Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science, 286, 525-528. Go to original source... Go to PubMed...
    27. Zhang G., Wu H., Shi J., Ganz T., Ross C.R., Blecha F. (1998): Molecular cloning and tissue expression of porcine betadefensin-1. FEBS Letters, 424, 37-40. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content