Czech Journal of Animal Science, 2017 (vol. 62), issue 3
Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kineticsOriginal Paper
Dasen Liu, Yanfang Li, Guangliang Zhang, Peng Zhang, Penghua Wu, Shai Wang, Xiuxiu Wang
Czech J. Anim. Sci., 2017, 62(3):89-97 | DOI: 10.17221/94/2015-CJAS 
The objective of this study was to investigate the relationship between the protein secondary structure and the protein rumen degradation kinetics and the protein fractions of mixed feedstuffs of soybean meal with distillers dried grains with solubles (DDGS) at five mixed ratios (DDGS to soybean meal: 100 : 0, 70 : 30, 50 : 50, 30 : 70, 0 : 100). The Fourier transform infrared (FTIR) as a novel and cheap approach was used to detect the protein secondary structure, and the in situ nylon bag method was used to measure degradation kinetics of protein. Protein fractions were classified based on the Cornell net carbohydrate protein system. The...
Effect of opioid receptors agonists on feeding behaviour using different diets in ad libitum fed neonatal chickenOriginal Paper
Shoresh Arva, Morteza Zendehdel, Yahya Ebrahim Nezhad, Jamshid Ghiasi Ghalehkandi, Shahin Hassanpour, Habib Aghdam Shahryar
Czech J. Anim. Sci., 2017, 62(3):98-109 | DOI: 10.17221/2/2016-CJAS
Despite progress in studying the role of opioids in reward, the effect of opioid receptors on feeding behaviour in ad libitum fed meat-type chicken offered different diet types is still unclear. So in this study, 12 experiments (each included 4 groups) were designed to determine the role of μ, δ, and κ receptors with different diets on feeding responses in ad libitum fed neonatal chicken. In Experiment 1, group A chicken were intracerebroventricularly (ICV) injected with saline, groups B-D chicken were ICV injected with DAMGO (µ-opioid receptor agonist; 125, 250, and 500 pmol), then standard diet without fat was...
Effect of sex and dietary treatment on the composition and rheological properties of dry-cured ham subcutaneous fatOriginal Paper
José Francisco Segura Plaza, Rosa Escudero, María Dolores Romero de Ávila, lvaro Olivares, María Isabel Cambero, Clemente José López-Bote
Czech J. Anim. Sci., 2017, 62(3):110-120 | DOI: 10.17221/18/2016-CJAS
The effect of sex, dietary fat source (lard vs palm oil), and glycerol inclusion in fattening diet on the composition, fatty acid distribution within the triglyceride (TAG) and slip point and textural parameters was studied on dry-cured hams subcutaneous fat. A marked effect of sex on saturated fatty acids (SFA) percentage was found with barrows showing higher values than gilts. No effect of dietary fat source on subcutaneous SFA or polyunsaturated fatty acids (PUFA) was observed. Dietary glycerol increased monounsaturated fatty acids and decreased total PUFA in subcutaneous fat. Besides, the possibility of altering fatty acid composition at the 2-position...
Migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantationOriginal Paper
Hilal Güralp, Kseniia Pocherniaieva, Miroslav Blecha, Tomá¹ Policar, Martin P¹enièka, Taiju Saito
Czech J. Anim. Sci., 2017, 62(3):121-129 | DOI: 10.17221/40/2016-CJAS
Pikeperch Sander lucioperca is a valuable fish in Europe, and basic information about its embryonic development, especially primordial germ cell (PGC) migration, is important for use in biotechnology. We categorized pikeperch embryonic development into six stages as in other fish species: zygote, cleavage, blastula, gastrula, segmentation, and hatching and described PGC migration. PGCs were visualized by injection of synthesized green fluorescent protein (GFP) within the 3'untranslated region (UTR) mRNA of nanos3. GFP-positive PGCs appeared in all embryos at approximately 100% epiboly. Time-lapse imaging revealed the PGC migration...
Effect of salsolinol on ACTH and cortisol response to handling stress in early anestrous sheepOriginal Paper
Ma³gorzata Hasiec, Micha³ Szlis, Natalia Chmielewska, Konrad Górski, Katarzyna Romanowicz, Tomasz Misztal
Czech J. Anim. Sci., 2017, 62(3):130-139 | DOI: 10.17221/41/2016-CJAS
Inhibition of hypothalamic-pituitary-adrenal (HPA) axis activity by salsolinol was demonstrated in lactating sheep. We assessed whether salsolinol regulates, besides lactation, also adrenocorticotropic hormone (ACTH) and cortisol release, and if its action is prolactin-dependent. We examined two groups of early anestrous sheep, which received for three days salsolinol or vehicle-only intracerebroventricular injections, and a group of lactating sheep injected with the vehicle only. On day 3, blood samples were collected for over six hours and the anterior pituitary was dissected. Plasma ACTH, cortisol, and prolactin concentrations, and proopiomelanocortin...