Czech J. Anim. Sci., 2017, 62(3):121-129 | DOI: 10.17221/40/2016-CJAS

Migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantationOriginal Paper

Hilal Güralp*, Kseniia Pocherniaieva, Miroslav Blecha, Tomá¹ Policar, Martin P¹enièka, Taiju Saito
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Èeské Budìjovice, Vodòany, Czech Republic

Pikeperch Sander lucioperca is a valuable fish in Europe, and basic information about its embryonic development, especially primordial germ cell (PGC) migration, is important for use in biotechnology. We categorized pikeperch embryonic development into six stages as in other fish species: zygote, cleavage, blastula, gastrula, segmentation, and hatching and described PGC migration. PGCs were visualized by injection of synthesized green fluorescent protein (GFP) within the 3'untranslated region (UTR) mRNA of nanos3. GFP-positive PGCs appeared in all embryos at approximately 100% epiboly. Time-lapse imaging revealed the PGC migration pattern from their initial appearance to location at the gonadal ridge. We conducted blastomere transplantation (BT) at the blastula stage. Donor embryos were labelled with GFP-nos3 3'UTR mRNA and tetramethylrhodamine dextran to label PGCs and somatic cells, respectively. Twelve BT chimeras were produced, with eight surviving to hatching. All exhibited donor-derived somatic cells in the developing body. The PGCs from donor embryos were observed to migrate towards the gonad region of the host embryos. Our results indicated that BT can be successfully applied in pikeperch, and these findings may be useful to produce germline chimeras in percids.

Keywords: germ cell transplantation; germ cell migration; germ-line chimera; nanos; primordial germ cell visualization

Published: March 31, 2017  Show citation

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Güralp H, Pocherniaieva K, Blecha M, Policar T, P¹enièka M, Saito T. Migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation. Czech J. Anim. Sci. 2017;62(3):121-129. doi: 10.17221/40/2016-CJAS.
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    References

    1. Boldajipour B., Mahabaleshwar H., Kardash E., ReichmanFried M., Blaser H., Minina S., Wilson D., Xu Q., Raz E. (2008): Control of chemokine-guided cell migration by ligand sequestration. Cell, 132, 463-473. Go to original source... Go to PubMed...
    2. Doitsidou M., Reichman-Fried M., Stebler J., Koprunner M., Dorries J., Meyer D., Esguerra C.V., Leung T., Raz E. (2002): Guidance of primordial germ cell migration by the chemokine SDF-1. Cell, 111, 647-659. Go to original source... Go to PubMed...
    3. Fujimoto T., Kataoka T., Sakao S., Saito T., Yamaha E., Arai K. (2006): Developmental stages and germ cell lineage of the loach (Misgurnus anguillicaudatus). Zoological Science, 23, 977-989. Go to original source... Go to PubMed...
    4. Guralp H., Pocherniaieva K., Blecha M., Policar T., Psenicka M., Saito T. (2016): Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation. Czech Journal of Animal Science, 61, 273-280. Go to original source...
    5. Hilge V., Steffens W. (1996): Aquaculture of fry and fingerling of pike-perch (Stizostedion lucioperca L.) - a short review. Journal of Applied Ichthyology, 12, 167-170. Go to original source...
    6. Koprunner M., Thisse C., Thisse B., Raz E. (2001): A zebrafish nanos-related gene is essential for the development of primordial germ cells. Genes and Development, 15, 2877-2885. Go to original source... Go to PubMed...
    7. Kristan J., Alavi S.M.H., Stejskal V., Policar T. (2013): Hormonal induction of ovulation in pikeperch (Sander lucioperca L.) using human chorionic gonadotropin (hCG) and mammalian GnRH analogue. Aquaculture International, 21, 811-818. Go to original source...
    8. Lacerda S.M.S.N., Costa G.M.J., Campos-Junior P.H.A., Segatelli T.M., Yazawa R., Takeuchi Y., Morita T., Yoshizaki G., Franca L.R. (2013): Germ cell transplantation as a potential biotechnological approach to fish reproduction. Fish Physiology and Biochemistry, 39, 3-11. Go to original source... Go to PubMed...
    9. Li M., Tan X., Jiao S., Wang Q., Wu Z., You F., Zou Y. (2015): A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3. Development Genes and Evolution, 225, 195-206. Go to original source... Go to PubMed...
    10. Linhartova Z., Saito T., Psenicka M. (2014): Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca). Journal of Applied Ichthyology, 30, 29-39. Go to original source...
    11. Nelson J.S. (2006): Fishes of the World. John Willey and Sons, Hoboken, USA.
    12. Okutsu T., Shikina S., Kanno M., Takeuchi Y., Yoshizaki G. (2007): Production of trout offspring from triploid salmon parents. Science, 317, 1517. Go to original source... Go to PubMed...
    13. Psenicka M., Saito T., Linhartova Z., Gazo I. (2015): Isolation and transplantation of sturgeon early-stage germ cells. Theriogenology, 83, 1085-1092. Go to original source... Go to PubMed...
    14. Psenicka M., Saito T., Rodina M., Dzyuba B. (2016): Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells. Cryobiology, 72, 119-122. Go to original source... Go to PubMed...
    15. Richardson B.E., Lehmann R. (2010): Mechanisms guiding primordial germ cell migration: strategies from different organisms. Nature Reviews Molecular Cell Biology, 11, 37-49. Go to original source... Go to PubMed...
    16. Saito T., Otani S., Fujimoto T., Suzuki T., Nakatsuji T., Arai K., Yamaha E. (2004): The germ line lineage in ukigori, Gymnogobius species (Teleostei: Gobiidae) during embryonic development. The International Journal of Developmental Biology, 48, 1079-1085. Go to original source... Go to PubMed...
    17. Saito T., Fujimoto T., Maegawa S., Inoue K., Tanaka M., Arai K., Yamaha E. (2006): Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA. The International Journal of Developmental Biology, 50, 691-700. Go to original source... Go to PubMed...
    18. Saito T., Goto-Kazeto R., Arai K., Yamaha E. (2008): Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation. Biology of Reproduction, 78, 159-166. Go to original source... Go to PubMed...
    19. Saito T., Goto-Kazeto R., Fujimoto T., Kawakami Y., Arai K., Yamaha E. (2010): Inter-species transplantation and migration of primordial germ cells in cyprinid fish. The International Journal of Developmental Biology, 54, 1479-1484. Go to original source... Go to PubMed...
    20. Saito T., Goto-Kazeto R., Kawakami Y., Nomura K., Tanaka H., Adachi S., Arai K., Yamaha E. (2011): The mechanism for primordial germ-cell migration is conserved between Japanese eel and zebrafish. PLoS ONE, 6, e24460. Go to original source... Go to PubMed...
    21. Saito T., Psenicka M., Goto R., Adachi S., Inoue K., Arai K., Yamaha E. (2014): The origin and migration of primordial germ cells in sturgeons. PLoS ONE, 9, e86861. Go to original source... Go to PubMed...
    22. Saito T., Kurokawa Y., Takiguchi S., Miyazaki Y., Takahashi T., Yamasaki M., Miyata H., Nakajima K., Mori M., Doki Y. (2015): Accuracy of multidetector-row CT in diagnosing lymph node metastasis in patients with gastric cancer. European Radiology, 25, 368-374. Go to original source... Go to PubMed...
    23. Shikina S., Nagasawa K., Hayashi M., Furuya M., Iwasaki Y., Yoshizaki G. (2013): Short-term in vitro culturing improves transplantability of type A spermatogonia in rainbow trout (Oncorhynchus mykiss). Molecular Reproduction and Development, 80, 763-773. Go to original source... Go to PubMed...
    24. Takeuchi Y., Yoshizaki G., Takeuchi T. (2001): Production of germ-line chimeras in rainbow trout by blastomere transplantation. Molecular Reproduction and Development, 59, 380-389. Go to original source... Go to PubMed...
    25. Wang X., Liu Q., Xiao Y., Yang Y., Wang Y., Song Z., You F., An H., Xiao Z., Xu S., Ma D., Li J. (2015): The dnd RNA identifies germ cell origin and migration in olive flounder (Paralichthys olivaceus). BioMed Research International, 2015, Article ID 428591. Go to original source...
    26. Weidinger G., Wolke U., Koprunner M., Klinger M., Raz E. (1999): Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells. Development, 126, 5295-5307. Go to original source... Go to PubMed...
    27. Yamaha E., Murakami M., Hada K., Otani S., Fujimoto T., Tanaka M., Sakao S., Kimura S., Sato S., Arai K. (2003): Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGC (primordial germ cell)-containing graft. Genetica, 119, 121-131. Go to original source... Go to PubMed...
    28. Yoshizaki G., Tago Y., Takeuchi Y., Sawatari E., Kobayashi T., Takeuchi T. (2005): Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae. Biology of Reproduction, 73, 88-93. Go to original source... Go to PubMed...

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