Czech Journal of Animal Science, 2016 (vol. 61), issue 6
A simple method for assessing hyaluronic acid production by cumulus-oocyte complexesOriginal Paper
K. Zámostná, J. Nevoral, T. Kott, R. Procházka, M. Orsák, M. Šulc, V. Pajkošová, V. Pavlík, T. Žalmanová, K. Hošková, F. Jílek, P. Klein
Czech J. Anim. Sci., 2016, 61(6):251-261 | DOI: 10.17221/51/2015-CJAS 
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such it is a possible biomarker of the in vitro maturing oocytes quality. Cumulus expansion is usually assessed by non-invasive methods based on visual evaluation with many inaccuracies. On the other hand, analytical measurement of the quantity of hyaluronic acid (HA), the most abundant compound of expanded cumuli, is one of possible methods to evaluate cumulus expansion precisely. Therefore, this study aimed to verify the applicability of HA analysis for evaluating the cumulus expansion and testing oocyte maturation. The COCs...
Influence of the addition of exogenous xylanase with or without pre-incubation on the in vitro ruminal fermentation of three fibrous feedsOriginal Paper
M.M.Y. Elghandour, A.E. Kholif, J. Hernández, M.D. Mariezcurrena, S. López, L.M. Camacho, O. Márquez, A.Z.M. Salem
Czech J. Anim. Sci., 2016, 61(6):262-272 | DOI: 10.17221/52/2015-CJAS
The effects of the exogenous fibrolytic enzyme (ENZ) commercial preparation Dyadic® xylanase PLUS (Dyadic International, Inc., Jupiter, USA), containing endo-1,4-β-d-xylanase, on ruminal fermentation of maize stover, oat straw, and sugarcane bagasse were examined using the in vitro gas production (GP) technique. The ENZ commercial preparation was added at 0 (control), 60 (low), 120 (medium), and 240 (high) µg/g dry matter of substrate, and at two times of application (direct addition just before fermentation or with a 72-h pre-incubation before fermentation). Ruminal GP volumes were recorded at 2, 4, 6, 8, 10, 12,...
Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulationOriginal Paper
H. Güralp, K. Pocherniaieva, M. Blecha, T. Policar, M. Pšenička, T. Saito
Czech J. Anim. Sci., 2016, 61(6):273-280 | DOI: 10.17221/35/2015-CJAS
Recently, transplantation of germ cells has attracted attention as a potential technique for efficient reproduction of fish. One of the well-proven techniques to deliver donor germ cells into a recipient is the transplantation of primordial germ cells (PGCs) during the blastula stage. Nevertheless, the application of such techniques so far has been limited to model fish species such as zebrafish, due to the lack of information about early development in many fish species. We propose that pikeperch (Sander lucioperca) can be a useful model species for establishing this technique in the order Perciformes, which includes commercially and ecologically...
Short-term supplementation with dietary fructooligosaccharide and dietary mannitol elevated the absorption of calcium and magnesium in adult ratsOriginal Paper
J. Xiao, E. Sakaguchi, G. Bai
Czech J. Anim. Sci., 2016, 61(6):281-289 | DOI: 10.17221/44/2015-CJAS
The effects of dietary fructooligosaccharide (FOS) and dietary mannitol on the absorption of Ca and Mg in a short term feeding trial were studied. Adult Wistar rats were divided into three groups and fed diets containing 0, 8% FOS or 8% mannitol for seven days. Daily intake and feces were monitored for three days to determine the apparent absorption of Ca and Mg. At the last day of the feeding trial, blood sample was collected from cecal vein to assess Ca and Mg levels. The cecum and colon were removed to analyze the parameters. The results showed that both dietary FOS and dietary mannitol significantly increased the apparent absorptions of Ca and...
A chromosome-wide QTL mapping on chromosome 2 to identify loci affecting live weight and carcass traits in F2 population of Japanese quailOriginal Paper
E. Nasirifar, M. Talebi, A. Esmailizadeh, H. Moradian, S.S. Sohrabi, N. Askari
Czech J. Anim. Sci., 2016, 61(6):290-297 | DOI: 10.17221/113/2014-CJAS
The Japanese quail (Coturnix japonica) is a considerable species which is often used for animal modelling in breeding researches. This study aims to detect quantitative trait loci (QTL) underlying growth and carcass traits in Japanese quail. A three-generation resource population was developed using wild and white Japanese quail strains. The total mapping population consisted of 472 birds. Eight pairs of white and wild birds were mated reciprocally and 34 F1 birds were generated. The F1 birds were intercrossed to produce F2 offspring (422 birds). All of the animals from three generations were genotyped for...