Czech Journal of Animal Science, 2016 (vol. 61), issue 5
Benefits of hormone treatment of both sexes in semi-artificial reproduction of pikeperch (Sander lucioperca L.)Original Paper
M. Blecha, A.M. Samarin, J. Křišťan, T. Policar
Czech J. Anim. Sci., 2016, 61(5):203-208 | DOI: 10.17221/60/2015-CJAS 
Propagation of pikeperch Sander lucioperca by semi-artificial management was investigated using hormonal induction to better understand the effect of hormonal treatment of both sexes. Fourteen pairs of pikeperch brood fish were divided into two groups (seven pairs in each group). Seven females in Group A and both sexes in Group B were hormonally injected with 500 IU of hCG per kg. Each pair of brood fish was held separately in a 300-l circular tank with an added spawning material on the bottom. Brood fish were removed from the tanks on the day after spawning; three-hundred randomly selected eggs were removed from the spawning nests and transferred...
Genetic variation, association analysis, and expression pattern of SMAD3 gene in Chinese cattleOriginal Paper
T. Shi, Y. Xu, M.-J. Yang, Y. Zhou, M. Liu, X.-Y. Lan, C.-Z. Lei, X.-L. Qi, F.-P. Lin, Y.-Y. Bai, H. Chen
Czech J. Anim. Sci., 2016, 61(5):209-216 | DOI: 10.17221/34/2015-CJAS
SMAD3, a member of SMAD transcription factors, plays a key role in transforming growth factor-beta (TGF-β) signalling pathway and regulation of muscle growth. However, there was no strong evidence of association between SMAD3 polymorphisms and body traits in animals. In this study, single nucleotide polymorphisms (SNPs) in SMAD3 gene were detected in four Chinese cattle breeds (Qinchuan, Jiaxian, Nanyang, and Caoyuan) by using DNA pool sequencing and PCR-RFLP, and their effects on gene expression and growth traits were evaluated in Qinchuan cattle. The results showed that four novel SNPs (NC_007308.5 c.-2017A>G, g.101664C>G,...
Relationship between muscle fibre characteristics and meat sensory properties in three nutria (Myocastor coypus) colour typesOriginal Paper
E. Tůmová, D. Chodová, L. Uhlířová, J. Vlčková, Z. Volek, V. Skřivanová
Czech J. Anim. Sci., 2016, 61(5):217-222 | DOI: 10.17221/59/2015-CJAS
The study determined the muscle fibre characteristics and sensory properties of the longissimus lumborum muscle (LL) of eight-month-old nutrias of three colour types and the correlations between meat histochemical measurements and eating quality. The following nutria colour types were used in the experiment: Standard (ST), Moravian Silver (MS), and Prestice Multicolour (PM). The nutria colour type significantly (P < 0.001) affected the number of type IIB fibres with the lowest number in ST (108 per mm2). The proportion of type I fibres varied between 10.6 and 14.2%; type IIA fibres varied between 6.1 and 7.8%; and type...
Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditionsOriginal Paper
M. Alhussien, P. Manjari, A.A. Sheikh, S. Mohammed Seman, S. Reddi, A.K. Mohanty, J. Mukherjee, A.K. Dang
Czech J. Anim. Sci., 2016, 61(5):223-231 | DOI: 10.17221/63/2015-CJAS
The goal of this work was to understand how polymorphonuclear leukocytes (PMN), the first line of cellular defence, integrate and prioritize multiple chemotactic signals to navigate during periods of demand like subclinical mastitis (SCM), clinical mastitis (CM), pregnancy (P), and calving (C). For this, blood and milk samples were collected from five groups of crossbred cows (healthy, with SCM, CM, pregnant, and newly calved). Maximum viability was seen in the PMN of healthy cows, whereas minimum viability was observed in CM cows. Phagocytic activity (PA) of blood and milk PMN decreased significantly (P < 0.05) at C and in CM cows. Chemotactic...
Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bullsOriginal Paper
D. Robakowska-Hyżorek, R.R. Starzyński, B. Żelazowska, J. Oprządek, T. Sadkowski, L. Zwierzchowski
Czech J. Anim. Sci., 2016, 61(5):232-242 | DOI: 10.17221/118/2014-CJAS
The aim of the study was to characterize nucleotide sequence polymorphisms in the bovine PTPRQ gene, to search for their possible effect on the expression of myogenic factor 6 (MYF6; MRF4) and myogenic factor 5 (MYF5) genes in skeletal muscle and on meat production traits. Three novel SNPs were found in intron 35 of the bovine PTPRQ gene: g.200,451A>G, g.200,467T>C, g.200,480C>T (GenBank Acc. No. NW_001494990.2; counted from translation initiation site). These SNPs are placed very closely to each other (within 29 base pairs). The results showed that genotype influenced the expression of MYF6...
Expression analysis of the porcine Homeobox A11 gene and its association with litter size in Large White pigsOriginal Paper
R. Lin, H. Jia, S. Chen, S. Zhao
Czech J. Anim. Sci., 2016, 61(5):243-249 | DOI: 10.17221/127/2014-CJAS
The homeobox A11 (HOXA11) gene, a well-known transcriptional regulator, plays a critical role in normal development of the uterus. In this study, the porcine HOXA11 gene, including its promoter region, was cloned and sequenced (GenBank Accession No. KF724933). Differential expression levels of HOXA11 in the endometrium at one stage of the estrous cycle and three stages of gestation for Meishan and Large White pigs were investigated. Association between the single nucleotide polymorphism (SNP) and litter size was conducted. Results suggested that expression of the HOXA11 gene was the highest on day 15 and...