Czech Journal of Animal Science, 2012 (vol. 57), issue 8

Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis

D. Kaczmarczyk, M. Luczynski, P. Brzuzan

Czech J. Anim. Sci., 2012, 57(8):345-352 | DOI: 10.17221/6269-CJAS  

There are two stocks of American paddlefish Polyodon spathula in Poland, one in Pogorze and the other in Wasosze. These stocks were established from small quantities of eggs imported from the USA in 1995. In this study, we examined genetic variation at seven microsatellite loci in adult fish from the two Polish farms and one stock farmed in Gorny Tykich in Ukraine. Our data were compared with those reported for one native population from the Mississippi River (USA). The polymorphism of examined loci varied in the Polish and Ukrainian stocks, showing 25-30 alleles across each stock (3.6-4.3 alleles per locus), though they were less polymorphic...

Relationship between milk yield, stage of lactation, and some blood serum metabolic parameters of dairy cows

A. Jóźwik, N. Strzałkowska, E. Bagnicka, W. Grzybek, J. Krzyżewski, E. Poławska, A. Kołataj, J.O. Horbańczuk

Czech J. Anim. Sci., 2012, 57(8):353-360 | DOI: 10.17221/6270-CJAS  

The aim of the study was to evaluate the effect of milk yield and stage of lactation on the activity of liver enzymes, cholesterol, and vitamin C concentration in blood of milking cows. The experiment was carried out on Polish Holstein-Friesian Black and White dairy cows with two different milk yield levels: M - medium (about 7000 kg per lactation) and H - high (about 10 000 kg per lactation). In blood serum, AST, ALT, GGT, CHOL, and vitamin C were estimated. The AST and ALT activities in the blood serum were lower in M group than in H group, however within M and H groups there were no differences in both aminotransferases activity between the 60th...

Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

L. Pavlata, L. Mišurová, A. Pechová, R. Dvořák

Czech J. Anim. Sci., 2012, 57(8):361-369 | DOI: 10.17221/6271-CJAS  

The goal of the experiment was to compare the effect of four different forms of selenium (Se) - sodium selenite (SS), lactate-protein selenium complex (SL), selenium enriched yeast (SY), and selenium-proteinate (SP) supplemented to pregnant goats on Se concentration and glutathione peroxidase (GSH-Px) activity in the blood of goats on the day of delivery and also on Se concentration and GSH-Px activity in the blood of newborn kids. The experiment involved 33 pregnant goats of White Short-haired breed. The supplementation started 6 weeks before the parturition. The goats were divided into 5 groups: control group C, not supplemented, and 4 trial groups...

Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines

L. Jarinkovičová, L. Máchal, J. Máchal, R. Filipčík, E. Tůmová, R. Horský

Czech J. Anim. Sci., 2012, 57(8):370-376 | DOI: 10.17221/6272-CJAS  

Ejaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate parameters were determined: sperm motility, concentrations of sperm cells, ejaculate volume. Sperm morphology was examined. In the blood samples, concentrations of glucose, cholesterol, creatinine, calcium, phosphorus, and magnesium were analysed. The motility of spermatozoa of the cocks was 47.0% (BPR), 47.7% (RIR), and 48.3% (SU), respectively. The highest volume of ejaculate was found in BPR...

Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

M. Ziaullah, A. Ijaz, M. Aleem, A.K. Mahmood, H. Rehman, S. Bhatti, U. Farooq, M.U. Sohail

Czech J. Anim. Sci., 2012, 57(8):377-381 | DOI: 10.17221/6273-CJAS  

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at -196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge,...

Estimation of genetic parameters of reproductive traits in Zandi sheep using linear and threshold models

H. Mohammadi, M. Moradi Shahrebabak, H. Moradi Sharebabak, M. Vatankhah

Czech J. Anim. Sci., 2012, 57(8):382-388 | DOI: 10.17221/6274-CJAS  

Genetic parameters for reproductive traits in Zandi sheep were estimated from data records of 5025 lambs from 178 sires and 1967 dams collected between the years 1993 and 2010 using ASReml statistical package. Genetic parameters were estimated for four basic and two composite traits. Year of lambing and age of ewe were used as fixed effects. Due to repeated record of ewe animal direct genetic effects and permanent environmental effects were considered random. The heritability estimates for conception rate, number of lambs born, number of lambs born alive, number of lambs alive at weaning, number of lambs born per ewe exposed, and number of lambs weaned...