Czech J. Anim. Sci., 2012, 57(8):377-381 | DOI: 10.17221/6273-CJAS

Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

M. Ziaullah1, A. Ijaz2, M. Aleem3, A.K. Mahmood1, H. Rehman2, S. Bhatti1, U. Farooq4, M.U. Sohail2
1 Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan
2 Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
3 Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan
4 University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at -196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.

Keywords: canine; butylated hydroxytoluene; cryopreservation; sperm quality

Published: August 31, 2012  Show citation

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Ziaullah M, Ijaz A, Aleem M, Mahmood AK, Rehman H, Bhatti S, et al.. Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm. Czech J. Anim. Sci. 2012;57(8):377-381. doi: 10.17221/6273-CJAS.
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