Czech Journal of Animal Science, 2011 (vol. 56), issue 5
Heritabilities and genetic correlations of lactational and daily somatic cell score with conformation traits in Polish Holstein cattle
E. Ptak, W. Jagusiak, A. Żarnecki, A. Otwinowska-Mindur
Czech J. Anim. Sci., 2011, 56(5):205-212 | DOI: 10.17221/1432-CJAS 
The objective of this study was to estimate heritabilities and genetic correlations of lactational and daily somatic cell scores with descriptive and linear type traits in Polish Holstein-Friesian cows. Data were: test-day somatic cell scores and conformation evaluations of 24 599 primiparous cows, daughters of 802 sires. Cows calved from 2006 to 2007. The lactational somatic cell score was calculated as the average of four test-day somatic cell scores at least. The daily somatic cell score was the test-day somatic cell score closest to the date of type evaluation. A multi-trait animal model was used to estimate genetic parameters. (Co)variance components...
Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
M. Nesvadbová, A. Knoll
Czech J. Anim. Sci., 2011, 56(5):213-216 | DOI: 10.17221/1428-CJAS
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each experiment because the gene expression level may be changed in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression of stability of...
Analysis of Czech cold-blooded horses: genetic parameters, breeding value and the influence of inbreeding depression on linear description of conformation and type characters
L. Vostrý, Z. Čapková, J. Přibyl, K. Mach
Czech J. Anim. Sci., 2011, 56(5):217-230 | DOI: 10.17221/1430-CJAS
Genetic parameters, breeding values and inbreeding depression for 22 linear type description of conformation and type characters and 4 body measurements were evaluated in a group of 1744 horses of three original cold-blooded breeds in the Czech Republic in a long period of 18 years (1990-2007). Based on the values of Akaike's information criterion, residual variance and heritability coefficient, a model with fixed effects (sex, year of description, breed, and classifier) and with a random effect (animal) was selected. Heritability coefficients for the particular traits were in the range of 0.11 to 0.55 and genetic correlations ranged from -0.63 to...
Effect of the type of non-fibre carbohydrates in grass silage-based diets on in sacco ruminal degradability and protein value of lupin (Lupinus angustifolius L. cv. Sonet) seeds ground to different particle sizes
B. Niwińska, M. Andrzejewski
Czech J. Anim. Sci., 2011, 56(5):231-241 | DOI: 10.17221/1431-CJAS
The study was conducted to find out the effects of the type of non-fibre carbohydrates (NFC) included in grass silage-based diets on in sacco degradability of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF) of lupin (Lupinus angustifolius L. cv. Sonet) seeds ground to different particle sizes. Measurements of degradability were carried out as a 3 × 2 × 3 × 3 experimental design of treatments with carbohydrates included in diets as primary sources of NFC (starch vs. pectin vs. sugars), particle size of ground seeds (1.0 vs. 5.7 mm), period of the trial (1 vs. 2 vs. 3) and cannulated cow (1 vs. 2 vs. 3). The variables...
Effect of genotype, age of hens and K/k allele on eggshell quality
Z. Ledvinka, L. Zita, M. Hubený, E. Tůmová, M. Tyller, P. Dobrovolný, M. Hruška
Czech J. Anim. Sci., 2011, 56(5):242-249 | DOI: 10.17221/1429-CJAS
We assessed the influence of the particular genotype, age of layers, feather growth-rate gene, and their mutual interactions on selected indicators of eggshell quality in six groups of hens of the laying type Dominant. The following genotypes were examined in the experiment: Barred Plymouth Rock, Dominant BPR 951 (K) strain, slow-feathering; Barred Plymouth Rock, Dominant BPR 901 (k) strain, fast-feathering; Blue Plymouth Rock, Dominant BLPR 954 (K) strain, slow-feathering; Blue Plymouth Rock, Dominant BLPR 894 (k) strain, fast-feathering; crossbreds of the above strains in the F1 generation Dominant D 107 blue (K), slow-feathering and Dominant...