Czech Journal of Animal Science, 2010 (vol. 55), issue 9
Effect of duodenal infusions of leucine on milk yield and plasma amino acids in dairy cows
M. Richter, J. Svobodová, L. Křížová, J. Třináctý, P. Homolka
Czech J. Anim. Sci., 2010, 55(9):351-358 | DOI: 10.17221/210/2009-CJAS 
Four high-yielding lactating Holstein cows fitted with duodenal cannulas were used in the experiment. Cows were divided into 2 groups - control (Control) with leucine deficiency and experimental (Leucine) with a leucine supplement. The experiment was divided into 4 periods of 7 days, each consisting of a 3-day preliminary period followed by a 4-day experimental period. In the first period, 2 cows were assigned to Control and the remaining 2 to Leucine. In the subsequent period the cows were switched to the other treatment. Cows were fed individually twice daily the basal diet based on maize silage, lucerne hay and supplemental mixture. Infusions of...
Ruminal degradability of dry matter and neutral detergent fibre of grasses
F. Jančík, V. Koukolová, P. Homolka
Czech J. Anim. Sci., 2010, 55(9):359-371 | DOI: 10.17221/211/2009-CJAS
A study was conducted (I) to determine rumen degradability (in sacco) of dry matter (DM) and neutral detergent fibre (NDF) of the most important grass species grown in the Czech Republic, (II) to compare grass species (n = 5) according to calculated degradation parameters, and (III) to establish prediction equations for degradation parameters from chemical composition. Forty samples of the most important grass species (Dactylis glomerata, Phleum pratense, Lolium perenne, Festuca arundinacea, Felina hybrid) were harvested in 2004 and 2005 and analyzed for chemical composition, and DM and NDF in sacco degradability....
Nutritive value of Trifolium pratense L. for ruminants estimated from in situ ruminal degradation of neutral detergent fibre and in vivo digestibility of organic matter and energy
V. Koukolová, P. Homolka, O. Koukol, F. Jančík
Czech J. Anim. Sci., 2010, 55(9):372-381 | DOI: 10.17221/304/2009-CJAS
Seven clover (Trifolium pratense L.) samples were collected at three different stages of the same sward (first growth (I), n = 3; first regrowth (II), n = 3; second regrowth (III), n = 1) during the growing season from 10th of May to 17th of August. Samples were analyzed for chemical composition, gross energy (GE) content, in vivo organic matter digestibility (OMD) and gross energy digestibility (GED) in sheep, and in situ rumen degradability of neutral detergent fibre (NDF). The contents of ash, crude protein (CP), crude fibre (CF), NDF, acid detergent fibre (ADF), acid detergent...
Effects of dietary threonine on growth performance and carcass traits of Yangzhou geese
S.R. Shi, Z.Y. Wang, J.M. Zou, H.M. Yang, N. Jiang
Czech J. Anim. Sci., 2010, 55(9):382-387 | DOI: 10.17221/172/2009-CJAS
A dose-response experiment with 5 total dietary threonine (Thr) levels (0.54, 0.64, 0.74, 0.84 and 0.94%) was conducted to study the effect of dietary Thr on growth performance and carcass traits of Yangzhou geese from 0 to 8 weeks of age. Three hundred 1-day-old Yangzhou goslings were randomly allocated to 15 pens with 20 birds (10 males and 10 females) per pen according to similar pen weight. There were 5 dietary treatments, consisting of 3 replicate pens. Weight gain, feed intake and feed/gain of geese from each pen were measured at 2-week intervals from 0 to 8 weeks. At 56 days of age, four geese (2 males and 2 females) were selected randomly from...
Selenium and α-tocopherol content in eggs produced by hens that were fed diets supplemented with selenomethionine, sodium selenite and vitamin E
M. Skřivan, I. Bubancová, M. Marounek, G. Dlouhá
Czech J. Anim. Sci., 2010, 55(9):388-397 | DOI: 10.17221/92/2010-CJAS
The effect of supplementing dietary selenium (Se) and vitamin E was investigated in 330 24-week-old laying hens. The hens were fed a basal diet containing Se and α-tocopherol at 0.11 and 26 mg/kg, respectively, or a diet supplemented with Se at 0.3 mg/kg and vitamin E between 0 and 625 mg/kg. Se was supplied as Se-methionine or sodium selenite. The eggs were collected for analysis during the third, seventh and eleventh weeks of the experiment. Supplementation of either form of Se significantly increased the Se concentration in egg yolks and whites, with a more pronounced effect caused by Se-methionine. The egg yolk α-tocopherol concentration...
Six single nucleotide polymorphisms in adipocyte fatty acid-binding protein (A-FABP) gene in Beijing ducks
N. Zhao, S.S. Hou, X.L. Liu, X.G. Yang, W. Huang
Czech J. Anim. Sci., 2010, 55(9):398-400 | DOI: 10.17221/1401-CJAS
PCR-SSCP was applied to analyze the polymorphisms of A-FABP gene in 4 lines of Beijing ducks (n = 400). The results showed that six SNPs were found in intron 3. There were no polymorphisms found in exon 3 or exon 4. The discovered SNPs were deposited in GenBank (Acc. No.: EU306611 and EU306610). The frequencies of haplotypes A/B in the Z4, Z2, Cherry Valley, Z4 × Z2 populations were 0.745/0.255, 0.764/0.236, 0.552/0.448, 0.672/0.328, respectively. The linkage disequilibrium was stated. The above described SNPs of A-FABP gene allow the incoming association analysis.