Czech Journal of Animal Science, 2010 (vol. 55), issue 2
Genome reprogramming during the first cell cycle in in vitro produced porcine embryos
I. Barnetová, K. Okada
Czech J. Anim. Sci., 2010, 55(2):49-57 | DOI: 10.17221/26/2009-CJAS 
Conflicting data still exist regarding the extent of paternal pronuclear DNA demethylation in one cell-stage mammalian embryos. Demethylation of paternal pronuclear DNA was observed in in vitro produced porcine zygotes, whereas in vitro produced embryos do not show any or only weak paternal genome demethylation. In our experiments, we have used and compared two in vitro techniques commonly used for in vitro embryo production (in vitro fertilization and intracytoplasmic sperm injection) and then we evaluated the extent of labelling in both these groups after 5-methylcytosine (5-MeC) or dimethyl H3/K9 labelling. We...
Iodine status in ewes with the intake of iodine enriched alga Chlorella
J. Trávníček, V. Kroupová, R. Konečný, M. Staňková, J. Šťastná, L. Hasoňová, M. Mikulová
Czech J. Anim. Sci., 2010, 55(2):58-65 | DOI: 10.17221/40/2009-CJAS
The effect of increased intake of iodine at different selenium intake was studied in three groups of lambing ewes consisting of five animals and in their lambs for 76 days (from day 4 to day 80 post partum). Iodine in blood plasma, milk and urine was determined by a modified method according to Sandell-Kolthoff. Mineral supplement contained iodine and selenium in the form of I- or Se-enriched alga Chlorella. The content of iodine and selenium per 1 kg DM of experimental diet was as follows: group G1 0.7 mg I and 0.2 mg Se, group G2 0.7 mg I and 0.4 mg Se, group G3 1.3 mg I and 0.4 mg Se. The increased intake of iodine was not accompanied...
Eggshell quality and calcium utilization in feed of hens in repeated laying cycles
H. Arpášová, M. Halaj, P. Halaj
Czech J. Anim. Sci., 2010, 55(2):66-74 | DOI: 10.17221/90/2009-CJAS
Hens of the laying hybrid Shaver Starcross 288 were used in two consequential experiments. The first experiment (for 10 months) was conducted on hens at the age from 18 to 60 weeks (1st cycle; n = 50) and moulted hens after 10 months laying from 70 to 110 weeks of age (2nd cycle; n = 50). For the second experiment (for 8 months) in the 1st cycle 18 weeks old hens were bought (n = 60) and hens from the first experiment after moulting were used in the 2nd and 3rd cycle (n = 32 and n = 28). During the experiments eggshell qualitative parameters and calcium utilisation...
Variation in fatty acids in chicken meat as a result of a lupin-containing diet
E. Straková, P. Suchý, I. Herzig, P. Hudečková, Š. Ivanko
Czech J. Anim. Sci., 2010, 55(2):75-82 | DOI: 10.17221/105/2009-CJAS
This study was designed to verify the effect of diets containing lupin meal on the composition of fat in meat from fattened broiler chickens. It follows from the results that an increasing level of lupin meal (E1 and E2) resulted in a gradual decrease in the average level of saturated fatty acids (SFAs) in fat in breast and thigh muscles from experimental chickens as compared to the control group. This decrease was characterized by a significant (P ≤ 0.05) to highly significant (P ≤ 0.01) reduction in the level of palmitic acid, which is the most common fatty acid. Diets containing lupin meal showed an increase in monounsaturated...
The influence of prolactin on bone mineral density (BMD) and some biochemical markers of ovariectomized rats
B. Dolińska, A. Suszka-Świtek, S. Dragan, F. Ryszka, R. Kołacz, B. Patkowska-Sokoła
Czech J. Anim. Sci., 2010, 55(2):83-88 | DOI: 10.17221/195/2009-CJAS
A model of postmenopausal osteoporosis was used in the study. 107 days after ovariectomy the animals were subcutaneously applied 1.0 IU PRL/kg BM or 1.0 IU calcitonin/kg BM for 7 days in single doses. The application of the preparations started again 80 days after the first application. After 194 days the animals were narcotized, blood was collected, and lumbar (L2-L4) and femoral bones were prepared. The material obtained was subjected to densitometric analysis of bone mineral density (BMD). In ovariectomized rats the loss of osseous tissue was observed only in the lumbar bone. The application of prolactin to ovariectomized rats caused an increase...