Czech Journal of Animal Science, 2009 (vol. 54), issue 3

The effect of feeding diets markedly differing in the proportion of grass and maize silages on bovine milk fat composition

E. Samková, M. Pešek, J. Špička, T. Pelikánová, O. Hanuš

Czech J. Anim. Sci., 2009, 54(3):93-100 | DOI: 10.17221/1679-CJAS  

Ten Czech Pied cows in the mid-lactation stage were fed diets based on grass silage and maize silage. The composition of milk fats differed. The proportions of even-chain saturated fatty acids (SFAs) up to C14:0 were insignificant and the content of C16:0 was significantly higher (P < 0.05) when feeding a diet based on maize silage, while the proportions of the individual polyunsaturated fatty acids (PUFAs) were significantly (except for C18:2) higher when feeding a diet based on grass silage. The total SFA proportions were 67.60 and 62.93% (P < 0.05) of maize and grass silages, respectively, while...

Mitochondrial D-loop sequence variation among autochthonous horse breeds in Croatia

A. Ivanković, J. Ramljak, M. Konjačić, N. Kelava, P. Dovč, P. Mijić

Czech J. Anim. Sci., 2009, 54(3):101-111 | DOI: 10.17221/1678-CJAS  

Genetic variation in three Croatian coldblood horse populations was analysed using a sequence analysis of the proximal part (nt 15 498-15 821) of the D-loop region of mtDNA. Twenty unrelated horses were chosen from the Posavina horse and the Croatian Coldblood breeds and fifteen horses from the Murinsulaner horse population. Sequencing of the proximal part of the mtDNA D-loop region revealed 26 polymorphic sites representing thirty haplotypes which were clustered into eight haplogroups. A wide variety of mitochondrial haplotypes of the analysed horse breeds were clustered into eight different haplogroups. Two haplogroups (D and F) were specific to...

Changes in methane release from organic matter passing through the digestive tract of horses

L. Kolář, M. Maršálek, J. Frelich, S. Kužel, P. Smetana, J. Zedníková, M. Švecová

Czech J. Anim. Sci., 2009, 54(3):112-120 | DOI: 10.17221/1677-CJAS  

Using the tests of methanogenic activity (TMA) changes in methane yield (YCH4) and anaerobic degradability (Dc) of organic matter of feeds and excrements were studied in an experimental group of six horses while complete analytical methods were applied (N-compound matters, proteins, non-protein N-compound matters, fat, nitrogen-free extract, ash, crude fibre, organic matter, NDF, ADF, hemicelluloses, cellulose, lignin and chemical oxygen demand COD) and the material balance was determined. The horses utilised 48.8% of organic matter of feeds in dry matter while the daily weight of droppings was 21 kg with 5.20% of dry matter and...

The effect of humic acid on zinc accumulation in chicken broiler tissues

I. Herzig, M. Navrátilová, J. Totušek, P. Suchý, V. Večerek, J. Blahová, Z. Zralý

Czech J. Anim. Sci., 2009, 54(3):121-127 | DOI: 10.17221/1676-CJAS  

Thirty-six selected male chickens were allocated into four groups C, HA, ZN, ZN + HA, and the experiment was initiated after 4 days of an adaptation period. Group C was fed a complete feed mixture without supplements. Group HA was fed the same diet with 500 mg of humic acid per chicken and day. Group ZN was loaded with 240 mg Zn (as 600 mg ZnSO4) per chicken and day, and birds of the group ZN + HA were loaded as those of the latter group, with additional 500 mg of humic acid per chicken and day. The treatments were carried out for 10 days. Subsequently, the chickens were slaughtered and samples of liver, kidneys, leg muscles and blood were...

DNA microsatellite analysis of genetic diversity among Chinese indigenous laying-type ducks (Anas platyrhynchos)

Y. Su, G.H. Chen

Czech J. Anim. Sci., 2009, 54(3):128-135 | DOI: 10.17221/1675-CJAS  

The genetic polymorphisms of 17 microsatellites were investigated in four indigenous laying-type duck breeds in China. The average number of alleles (Na) and average rates of homozygotes of each breed were counted. Accordingly, allele frequencies of the 17 microsatellites, polymorphism information content (PIC), mean heterozygosity (H) and genetic distances (Ds) were also calculated. Moreover, dendrograms using UPGMA and the neighbour-joining method were produced. The four breeds have a high average PIC (0.643) and H (0.682). Ds are between 0.514 and 0.662, the gene differentiation among the four breeds...