Czech Journal of Animal Science, 2008 (vol. 53), issue 8

Genetic diversity of five Chinese goat breeds assessed by microsatellite markers

J.Y. Li, H. Chen, X.Y. Lan, X.J. Kong, L.J. Min

Czech J. Anim. Sci., 2008, 53(8):315-319 | DOI: 10.17221/347-CJAS  

The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). Within the nine polymorphic loci, allele frequencies, number of effective alleles (Ne), heterozygosity (He), polymorphism information content (PIC), genetic identity (I) and Nei's standard genetic distance (D) were calculated, and UPGMA phylogenetic tree was constructed based on allele frequencies. The average number of alleles was 9.4, ranging from four to eleven at the nine assessed loci. The average values of Ne, He, PIC of all loci were 4.716, 0.765,...

Fibre and ergosterol contents in forage of Arrhenatherum elatius, Dactylis glomerata and Festulolium at the end of the growing seaso

J. Skládanka, V. Dohnal, A. Ježková

Czech J. Anim. Sci., 2008, 53(8):320-328 | DOI: 10.17221/346-CJAS  

The objective of this paper is to evaluate the contents of CF, NDF, ADF and ergosterol in the forage of Fistulolium, Dactylis glomerata and Arrhenatherum elatius from stands harvested at the end of the growing season. The contents of CF, NDF and ADF were analysed using the ANKOM Fibre Analyzer instrument. The ergosterol content was analysed by the HPLC method. The lowest CF content was in the Festulolium forage matter (26.8%). Conversely, the highest content was in the Arrhenatherum elatius forage (30.2%). The CF content gradually increased during autumn from 28.0% to 29.4%. Likewise, the NDF and ADF contents were lowest...

Effect of the stage of maturity on the leaf percentage of lucerne and the effect of additives on silage characteristics

Y. Tyrolová, A. Výborná

Czech J. Anim. Sci., 2008, 53(8):329-334 | DOI: 10.17221/345-CJAS  

The first part of the study concerns the effect of the stage of maturity on nutrient content and leaf percentage of lucerne (Medicago sativa). The plants of lucerne were harvested and analysed at several stages of growth. The crop yield and changes in the concentration of nutrients were determined. The leaf percentage from whole herbage and leaf yield were determined as well. The leaf percentage at the growth stage of small buds was significantly (P < 0.05) higher than at the growth stage of bloom in all three cuts (1st cut 52.7% vs. 46.62%; 2nd cut 52.03% vs. 44.70%; 3rd cut 50.58% vs. 46.26%). Crude...

A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling

M. Marciňáková, A. Lauková, M. Simonová, V. Strompfová, B. Koréneková, P. Naď

Czech J. Anim. Sci., 2008, 53(8):335-344 | DOI: 10.17221/348-CJAS  

A new probiotic, bacteriocin-producing strain of Enterococcus faecium EF9296 (isolate of our laboratory) was used in grass ensiling as an inoculant. The antimicrobial effect as well as the nutritive quality of silage was evaluated along with the aerobic stability test. Grass was ensiled with EF9296 strain (109 colony-forming units per gram of fresh material) and the silage without EF9296 was also prepared. The silages were processed in 1-litre plastic bottles. The samples were collected at days 0-1 (start of experiment), 7, 14 and 105 of ensiling to check the microbial status and chemical parameters of silage. At the end of ensiling...

The effects of space allowance on egg yield, egg quality and plumage condition of laying hens in battery cages

M. Sarica, S. Boga, U.S. Yamak

Czech J. Anim. Sci., 2008, 53(8):345-353 | DOI: 10.17221/349-CJAS  

This study was conducted to investigate the effects of cage stocking density on egg yield, some egg quality traits and plumage condition in laying hens. Eighteen weeks-old 264 ISA-Brown pullets were divided into four cage density groups. The densities were 2 000, 1 000, 667 and 500 cm2 per hen (by allocating 1, 2, 3 and 4 hens per cage; floor area 40 × 50 cm) with 48, 30, 24 and 21 replicate cages, totally 123 three-tier battery cages. During the experimental period from 18 to 53 weeks of age, all birds were provided illumination for 16 hours a day. The hens were fed a diet containing 11.7 MJ ME/kg and 180 g CP during the period of 18 to...