Czech J. Anim. Sci., 2008, 53(8):315-319 | DOI: 10.17221/347-CJAS

Genetic diversity of five Chinese goat breeds assessed by microsatellite markers

J.Y. Li1, H. Chen1,2, X.Y. Lan1, X.J. Kong1, L.J. Min3
1 College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A and F University, Yangling, Shaanxi, China
2 Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, China
3 Department of Animal Science, Qingdao Agricultural University, Qingdao, Shandong, China

The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). Within the nine polymorphic loci, allele frequencies, number of effective alleles (Ne), heterozygosity (He), polymorphism information content (PIC), genetic identity (I) and Nei's standard genetic distance (D) were calculated, and UPGMA phylogenetic tree was constructed based on allele frequencies. The average number of alleles was 9.4, ranging from four to eleven at the nine assessed loci. The average values of Ne, He, PIC of all loci were 4.716, 0.765, 0.732, respectively. The GZ population showed the highest variability (PIC = 0.78, He = 0.80). There was a relatively high level of genetic diversity in these goat breeds. A UPGMA diagram, based on Nei's standard genetic distances, yielded relationships between populations that agreed with what is known about their origin, history and geographical distribution.

Keywords: goat; microsatellites; biodiversity

Published: August 31, 2008  Show citation

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Li JY, Chen H, Lan XY, Kong XJ, Min LJ. Genetic diversity of five Chinese goat breeds assessed by microsatellite markers. Czech J. Anim. Sci. 2008;53(8):315-319. doi: 10.17221/347-CJAS.
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    References

    1. Belkhir K., Borsa P., Chikhi L., Raufaste N., Bonhomme F. (2000): GENETIX 4.02, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions CNRS UMR 5000, Université de Montpellier II, Montpellier, France.
    2. Botstein D., White R.L., Skolnick M., Davis R.W. (1980): Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32, 314-331.
    3. Buchanan F.C., Adams L.J., Litlejohn R.P., Maddox J.F., Crawford A.M. (1994): Determination of evolutionary relationships among sheep breeds using microsatellites. Genomics, 22, 397-403. Go to original source... Go to PubMed...
    4. Crawford A.M., Montgomery G.W., Pierson C.A., Brown T., Dodds K.G., Sunden S.L.F., Henry H.M., Ede A.J., Swarbrick P.A., Berryman T., Penty J.M., Hill D.F. (1994): Sheep linkage mapping: nineteen linkage groups derived from the analysis of paternal half-sib families. Genetics, 137, 573-579. Go to original source... Go to PubMed...
    5. Ellegren H., Moore S., Robinson N., Byrne K., Ward W., Sheldon B.C. (1997): Microsatellite evolution - a reciprocal study of repeat lengths at homologous loci in cattle and sheep. Mol. Biol. Evol., 14, 854-860. Go to original source... Go to PubMed...
    6. Iamartino D., Bruzzone A., Lanza A., Blasi M., Pilla F. (2005): Genetic diversity of Southern Italian goat populations assessed by microsatellite markers. Small Rumin. Res., 57, 249-255. Go to original source...
    7. Kemp S.J., Hishida O., Wambugu J., Rink A., Longeri M.L., Ma R.Z., Da J., Lewin H.A., Barendse W., Teale A.J. (1995): A panel of polymorphic bovine, ovine and caprine microsatellite markers. Anim. Genet., 26, 299-306. Go to original source... Go to PubMed...
    8. Lan X.Y., Pan C.Y., Chen H., Zhang C.L., Zhang A.L., Zhang L., Li J.Y., Lei C.Z. (2007): An MspI PCR-RFLP detecting a single nucleotide polymorphism at alphalactalbumin gene in goat. Czech J. Anim. Sci., 52, 138-142. Go to original source...
    9. Moore S.S., Sargeant L.L., King T.J., Mattick J.S., Georges M., Hetzel D.J.S. (1991): The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics, 10, 654-660. Go to original source... Go to PubMed...
    10. Mullenbach R., Lagoda P.J., Welter C. (1989): An efficient salt-chloroform extraction of DNA from blood and tissue. Trends Genetics, 5, 391.
    11. Nei M. (1973): Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA, 70, 3321-3323. Go to original source... Go to PubMed...
    12. Nei M. (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590. Go to original source... Go to PubMed...
    13. Saitbekova N., Gaillard C., Obexer-Ruff G., Dolf G. (1999): Genetic diversity in Swiss goat breeds based on microsatellite analysis. Anim. Genet., 30, 36-41. Go to original source... Go to PubMed...
    14. Vaiman D., Schibler L., Bourgeois F., Oustry A., Amigues Y., Cribiu E.P. (1996): A genetic linkage map of the male goat genome. Genetics, 144, 279-305. Go to original source... Go to PubMed...

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