Czech Journal of Animal Science, 2008 (vol. 53), issue 1

Effects of genistein and genistin on in vitro maturation of pig oocytes

Z. Vodková, R. Rajmon, J. Petr, P. Klabanová, F. Jílek

Czech J. Anim. Sci., 2008, 53(1):1-8 | DOI: 10.17221/2718-CJAS  

The objective of the study was to verify the hypothesis that GEN (genistein - phytoestrogen and an inhibitor of tyrosine protein kinase - TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 µg/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 µg/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours...

Efficiency of soy protein concentrate in diets of weaned piglets

J. Šiugždaité, A. Jerešiúnas, R. Stankevičius, J. Kulpys

Czech J. Anim. Sci., 2008, 53(1):9-16 | DOI: 10.17221/2719-CJAS  

The aim of this experiment was to determine the influence of "HP 300" soy protein concentrate (SPC) on the wellness, intestine microflora, growth rate and feed consumption compared with that of fish meal in weaned piglets. To create a balanced experiment on breed, gender, age and weight, two groups of 19 weaned piglets were composed. The first group was a control while the second group was experimental. The experiment was divided into two periods: the first period lasted for 27 days while the second lasted for 14 days. The duration of the experiment was 41 days in total. The composition of feed and sustenance were the same in both periods. The piglets...

Analysis of raw cow milk quality according to free fatty acid contents in the Czech Republic

O. Hanuš, J. Vegricht, J. Frelich, A. Macek, M. Bjelka, F. Louda, L. Janů

Czech J. Anim. Sci., 2008, 53(1):17-30 | DOI: 10.17221/2717-CJAS  

The concentration (c) of free fatty acids (FFAs) in milk is an indicator of dairy cow nutrition, milk straining, its bacterial contamination and storage quality. High FFA concentrations (cs) caused by lipolysis can damage the quality properties of milk products. Therefore the FFA content is introduced thanks to an increase in the efficiency of modern analytical methods as a milk quality indicator and as an indicator for its price as well. The goal of this paper was to analyse the FFA relations to the other milk quality indicators. The data set (n = 11 586) was evaluated by regression methods. In November and December the respective...

The effect of a diet supplemented with L-carnitine on egg production in pheasant (Phasianus colchicus)

P. Suchý, E. Straková, F. Vitula

Czech J. Anim. Sci., 2008, 53(1):31-35 | DOI: 10.17221/2720-CJAS  

The main aim of the study was to assess the effect of a diet supplemented with L-carnitine on egg weight and laying rate in hens of pheasant (Phasianus colchicus). The experiment lasted 14 weeks and was performed with 210 experimental and 210 control laying hens. The birds were kept in cages; one cock and seven hens per cage. Both control and experimental hens were administered the complete feed mixture, with the only difference that the feed mixture administered to experimental hens was supplemented with L-carnitine at a level of 0.01%. During the experimental period, eggs were collected and weight of individual eggs was determined. Control...

The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching

P. Szczerbik, T. Mikołajczyk, M. Sokołowska-Mikołajczyk, M. Socha, J. Chyb, P. Epler

Czech J. Anim. Sci., 2008, 53(1):36-44 | DOI: 10.17221/2721-CJAS  

The influence of cadmium (Cd) on the last stage of Prussian carp (Carassius auratus gibelio B.) oocyte maturation and embryonic development was investigated. The (postvitellogenic) oocytes were incubated with carp pituitary homogenate (Chh), 17α,20β-dihydroxy-4-pregnen-3-one (17α,20βOH-P) and Cd at the concentrations of 20, 100 and 200μM. Cd was found to stimulate spontaneous oocyte maturation, however it inhibited Chh-stimulated maturation. Cd had no influence on the effects of 17α,20βOH-P (MIS - maturation inducing steroid). It seems that the action of Cd on the last stage of oocyte maturation takes place...