Czech J. Anim. Sci., 2008, 53(1):36-44 | DOI: 10.17221/2721-CJAS

The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching

P. Szczerbik, T. Mikołajczyk, M. Sokołowska-Mikołajczyk, M. Socha, J. Chyb, P. Epler
Department of Ichthyobiology and Fisheries, University of Agriculture, Kraków, Poland

The influence of cadmium (Cd) on the last stage of Prussian carp (Carassius auratus gibelio B.) oocyte maturation and embryonic development was investigated. The (postvitellogenic) oocytes were incubated with carp pituitary homogenate (Chh), 17α,20β-dihydroxy-4-pregnen-3-one (17α,20βOH-P) and Cd at the concentrations of 20, 100 and 200μM. Cd was found to stimulate spontaneous oocyte maturation, however it inhibited Chh-stimulated maturation. Cd had no influence on the effects of 17α,20βOH-P (MIS - maturation inducing steroid). It seems that the action of Cd on the last stage of oocyte maturation takes place before the MIS production. In an in vivo study Prussian carp eggs were incubated in the water containing Cd at the concentrations of 0, 0.01, 0.1, 1 or 10 mg/l. There were no differences between the final numbers of hatched larvae in the respective groups, however Cd at the highest concentration accelerated hatching and increased the number of deformed larvae. These results suggest that Prussian carp eggs are very resistant to waterborne Cd.

Keywords: heavy metals; oocyte maturation; steroid hormones; embryonic development; hatching enzyme

Published: January 31, 2008  Show citation

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Szczerbik P, Mikołajczyk T, Sokołowska-Mikołajczyk M, Socha M, Chyb J, Epler P. The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching. Czech J. Anim. Sci. 2008;53(1):36-44. doi: 10.17221/2721-CJAS.
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    References

    1. Beattle J.H., Pascoe D. (1978): Cd uptake by rainbow trout, Salmo gairdneri eggs and alevin. J. Fish Biol., 13, 631-637. Go to original source...
    2. Beyersmann D., Hechtenberg S. (1997): Cd, gene regulation and cellular signalling in mammalian cells. Toxicol. Appl. Pharmacol., 144, 247-261. Go to original source... Go to PubMed...
    3. DiMichelle L., Taylor M.H., Singleton R. (1981): The hatching enzyme of Fundulus heteroclitus. J. Exp. Zool., 216, 133-140. Go to original source...
    4. Fisher K.C. (1963): The formation and properties of the external membrane of the trout egg. Roy. Soc. Canada, 1, 323-332.
    5. Gauthier C., Couture P., Pyle G.G. (2006): Metal effects on fathead minnows (Pimephales promelas) under field and laboratory conditions. Ecotoxicol. Environ. Saf., 63, 353-364. Go to original source... Go to PubMed...
    6. Hagenmaier H.E. (1972): Zum Schlupfprozess bei Fishen. II. Gewinnung und Charakterisierung des Schlupfsekretes bei der regenbogenforelle (Salmo gairdneri Rich.). Experimentia, 28, 1214-1215. Go to original source...
    7. Hallare A.V., Schirling M., Luckenbach T., Köhler H.R., Triebskorn R. (2005): Combined effects of temperature and Cd on developmental parameters and biomarker responses in zebrafish (Danio rerio) embryos. J. Therm. Biol., 30, 7-17. Go to original source...
    8. Kleiss-San Francisco S., Schuetz A. (1987): Sources of calcium and the involvement of calmodulin during steroidogenesis of the amago salmon (Onchorhynchus rhodurus). Gen. Comp. Endocrinol., 72, 39-53. Go to original source... Go to PubMed...
    9. Lohmann R.D., Beyersmann D. (1994): Effect of zinc and cadmium on apoptic DNA fragmentation in isolated bovine liver nuclei. Environ. Health Perspect., 102, 269-271. Go to original source... Go to PubMed...
    10. Łukjanienko W.J. (1974): Toksykologia ryb. PWRiL, Warszawa.
    11. Macia M., Hernández M. (1995): Modulation of the adherence of human polymorphonuclear leukocytes by Cd and nickel: sexual differences. Arch. Environ. Contam. Toxicol., 29, 15-19. Go to original source... Go to PubMed...
    12. Michibata H. (1981): Effect of water hardness on the toxicity of Cd to the eggs of the teleost Oryzias latipes. Bull. Environ. Contam. Toxicol., 27, 187-192. Go to original source... Go to PubMed...
    13. Mikołajczyk T., Weil C., Epler P., Breton B. (1990): Involvement of voltage-dependent calcium channels (VDCC) in the action of GnRH on GtH release in common carp (Cyprinus carpio L.): comparison with K+ action. Reprod. Nutr. Dev., 30, 619-628. Go to original source... Go to PubMed...
    14. Mi¶ J., Bigaj J. (1997): Hatching glands of carp (Cyprinus carpio L.) embryos from the eggs incubated at various concentrations of zinc or copper. Pol. Arch. Hydrobiol., 44, 153-155.
    15. Mi¶ J., Bieniarz K., Epler P., Sokołowska-Mikołajczyk M., Chyb J. (1995): Incubation of fertilized common carp (Cyprinus carpio L.) eggs in different concentrations of copper. Pol. Arch. Hydrobiol., 42, 269-276.
    16. Mi¶ J., Bieniarz K., Epler P., Sokołowska-Mikołajczyk M. (1996): Incubation of fertilized common carp (Cyprinus carpio L.) eggs in different concentrations of zinc. Pol. Arch. Hydrobiol., 43, 79-86.
    17. Mukherjee D., Guha D., Kumar V. (1992): Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro. Biomed. Environ. Sci., 5, 92-98.
    18. Mukherjee D., Kumar V., Chakraborti P. (1994): Effect of mercuric chloride and Cd chloride on gonadal function and its regulation in sexually mature common carp Cyprinus carpio. Biomed. Environ. Sci., 7, 13-24.
    19. Nagahama Y. (1990): Endocrine control of oocyte maturation in teleosts. In: Epple A., Scanes C.G., Stetson M.H. (eds.): Progress in Comparative Endocrinology. New York, Wiley-Liss, 385-392.
    20. Nagahama Y., Yoshikuni M., Yamashita M., Nagahama Y. (1994): Regulation of oocyte maturation in fish. In: Sherwood N.M, Hew C.L., Parrell A.P., Randall D.J. (eds.): Fish Physiology. Mol. Endocrinol. Fish New York, 13, 393-439. Go to original source...
    21. Nakagawa H., Ishio S. (1989): Effect of water hardness on the toxicity and accumulation of Cd in eggs of medaka Oryzias latipes. Nippon Suisan Gakkai Shi, 55, 321-326. Go to original source...
    22. Olsvik P.A., Gundersen P., Andersen R.A., Zachariasen K.E. (2000): Metal accumulation and metallothionein in two populations of brown trout, Salmo trutta, exposed to different natural water environments during a run-off episode. Aquat. Toxicol., 50, 301-316. Go to original source... Go to PubMed...
    23. O'Neill G.J. (1981): Effects of intraperitoneal lead and Cd on the humoral response of Salmo trutta. Bull. Environ. Comtam. Toxicol., 27, 42-48. Go to original source... Go to PubMed...
    24. Paksy K., Varga B., Lazar P. (1992): Cadmium interferes with steroid biosynthesis in rat granulosa and luteal cells in vitro. Biometals, 5, 245-250. Go to original source... Go to PubMed...
    25. Pundir R., Saxena A.B. (1992): Chronic toxic exposure of Cd on the pituitary gland of fish Puntius ticto and pattern of recoupment. J. Environ. Biol., 13, 69-74.
    26. Sarnowski P. (2000): Wpływ miedzi i kadmu na rozwój embrionalny pstr±ga tęczowego. Komunikaty Rybackie, 6, 30-31.
    27. Saxena M.P., Gopal K., Jones W., Ray P.K. (1992): Immune responses to Aeromonas hydrophila in catfish (Heteropneustis fossilis) exposed to Cd and hexchlorocyclohexane. Bull. Environ. Contam. Toxicol., 48, 194-201. Go to original source... Go to PubMed...
    28. Schoots A.F.M., Evertse P.A.C., Denuce J.M. (1983): Ultrastructural changes in hatching gland cells of pike embryos (Esox lucius L.) and evidence for their degeneration by apopresis. Cell Tissue Res., 229, 573-589. Go to original source... Go to PubMed...
    29. Singh H. (1989): Interaction of xenobiotics with reproductive endocrine functions in a protogynous teleost, Monopterus albus. Mar. Environ. Res., 28, 285-289. Go to original source...
    30. Singhal R.L., Vijayvargiya R., Shukla G.S. (1985): Toxic effects of cadmium and lead on reproductive functions. In: Thomas J.A., Korach K.S., McLachlan J.A. (eds.): Endocrine Toxicology. New York, Raven Press, 149-179.
    31. Sikorska J., Ługowska K. (2005): Wpływ kadmu na rozwój embrionalny karpia (Cyprinus carpio L.). Komunikaty Rybackie, 3, 6-8.
    32. Thomas P. (1990): Teleost model for studying the effects of chemicals on female reproductive endocrine function. J. Exp. Zool., 4, 126-128. Go to original source... Go to PubMed...
    33. Thomas P. (1993): Effect of cadmium on gonadotropin secretion from Atlantic croaker pituitaries incubated in vitro. Mar. Environ. Res., 35, 141-145. Go to original source...
    34. Tsang B.K., Carnegie J.A. (1983): Calcium requirement in the gonadotrophic regulation of rat granulosa cell progesterone production. Endocrinology, 113, 763-769. Go to original source... Go to PubMed...
    35. Tsang B.K., Carnegie J.A. (1984): Calcium dependent regulation of progesterone production: effects of the calcium ionophore A23187, prostaglandin E2, dl-isoproterenol and cholera toxin. Biol. Reprod., 30, 787-794. Go to original source... Go to PubMed...
    36. Van Der Kraak G. (1991): Role of calcium in the control of steroidogenesis in preowulatory ovarian follicles of the goldfish. Gen. Comp. Endocrinol., 81, 268-275. Go to original source... Go to PubMed...
    37. Veldhuis J.D., Klase P.A. (1982): Mechanisms by which calcium ions regulate the steroidogenic actions of luteinizing hormone in isolated ovarian cells in vitro. Endocrinology, 111, 1-6. Go to original source... Go to PubMed...
    38. Viarengo A. (1985): Biochemical effects of trace metals. Mar. Pollut. Bull., 16, 153-158. Go to original source...
    39. Victor B., Mahalingam S., Sarojini R. (1986): Toxicity of mercury and Cd on oocyte differentiation and vitellogenesis of the teleost, Lepidocephalichthys thermalis (Bleeker). J. Environ. Biol., 7, 209-214.
    40. Westernhagen H.V., Dethlefsen V. (1975): Combined effects of Cd and salinity on development and survival of flounder eggs. J. Marine Biol. Ass. UK, 55, 945- 957. Go to original source...
    41. Witeska M., Jezierska B., Chaber J. (1995): The influence of Cd on common carp embryos and larvae. Aquaculture, 129, 129-132. Go to original source...
    42. Wolf K., Quimby M.C. (1969): Fish cell and tissue culture. In: Hoar W.S., Randall D.J. (eds.): Fish Physiology. Academic Press, New York, 253-312. Go to original source...
    43. Woodworth J., Pascoe D. (1982): Cd toxicity to rainbow trout, Salmo gairdneri Richardson: a study of eggs and alevins. J. Fish Biol., 21, 47-57. Go to original source...
    44. Yamagami K. (1970): A method for rapid and quantitative determination of hatching enzyme (chorionase) activity of the medaka, Oryzias latipes. Annot. Zool. Jnp., 43, 1-9.
    45. Yamagami K. (1997): A short history of the hatching enzyme studies in medaka. Fish Biol. J. Medaka, 9, 5-15.
    46. Yamamoto T. (1960): Physiology of fertilization in fish eggs. Int. Rev. Cytol., 12, 361-405. Go to original source... Go to PubMed...
    47. Zotin A.I. (1958): The mechanism of hardening of the salmonid egg membrane after fertilization or spontaneous activation. J. Embryol. Exp. Morphol., 6, 546-568. Go to original source... Go to PubMed...

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